FastQCFastQC Report
Fri 10 Feb 2017
ERR1631569.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631569.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93310
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17981.9269102990033222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14631.5678919729932481No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12141.3010395456006858No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5690.6097953059693495No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.5347765512806772No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT3270.3504447540456543No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT3260.3493730575501018No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT2970.3182938591790805No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT2750.29471653627692634No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA2600.2786410888436395No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA2480.26578073089701No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA2420.2593505519236952No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC2290.2454184974815132No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC2260.24220340799485587No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA2190.23470153252598863No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT2150.2304147465437788No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.22612796056156897No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1960.21005251312828208No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT1950.2089808166327296No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT1910.20469403065051978No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.19290536919944273No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA1780.1907619762083378No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.18969027971278532No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT1740.18647519022612796No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT1550.16611295681063123No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1500.16075447433286894No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA1490.15968277783731646No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA1450.15539599185510664No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA1410.1511092058728968No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT1370.14682241989068698No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.14146393741292465No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT1320.14146393741292465No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT1300.13932054442181974No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA1270.13610545493516235No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.13610545493516235No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG1260.1350337584396099No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC1250.13396206194405744No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTA1250.13396206194405744No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT1230.13181866895295252No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT1200.12860357946629514No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT1190.12753188297074267No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT1190.12753188297074267No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA1190.12753188297074267No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG1130.12110170399742794No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.11467152502411317No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA1050.11252813203300824No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.11252813203300824No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT1040.11145643553745578No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1010.10824134605079841No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT1000.10716964955524595No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT990.1060979530596935No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.1060979530596935No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC960.10288286357303612No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAA200.001833282237.01
ATATGCT200.001833282237.035
ATGCCCC554.8748916E-1033.63636437
TGTTGAT250.00547081729.62
GTGTTGA250.00547081729.61
TATGCTG250.00547081729.636
ATACAAC250.00547081729.62
TATTGAC702.0190782E-1029.0714330
ATTGACT652.91584E-928.46153831
ACCGACC358.808204E-426.42857232
TTATTGC508.974765E-625.90000229
TATTGCT508.974765E-625.90000230
TTGACTG751.3396857E-824.66666632
ATTGCTG551.878485E-523.54545431
GTTGAAC400.001917989823.1250023
TGACTGT802.658453E-823.12500233
TGGGCAG4800.021.9687526
GGCAGTC4850.021.3608258
CAGTCGG4950.020.92929310
AGTCGGT4950.020.92929311
Produced by FastQC (version 0.11.5)