##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631569.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93310
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60629085842889	34.0	31.0	34.0	31.0	34.0
2	32.72369520951666	34.0	31.0	34.0	31.0	34.0
3	32.69750294716536	34.0	31.0	34.0	30.0	34.0
4	36.21796163326546	37.0	35.0	37.0	35.0	37.0
5	36.23547315400279	37.0	35.0	37.0	35.0	37.0
6	36.325956489122284	37.0	37.0	37.0	35.0	37.0
7	36.325581395348834	37.0	37.0	37.0	35.0	37.0
8	36.35064837637981	37.0	37.0	37.0	35.0	37.0
9	38.13716643446576	39.0	38.0	39.0	37.0	39.0
10	38.11338548922945	39.0	38.0	39.0	37.0	39.0
11	38.17090344014575	39.0	38.0	39.0	37.0	39.0
12	37.958182402743546	39.0	38.0	39.0	37.0	39.0
13	38.07786946736684	39.0	38.0	39.0	37.0	39.0
14	39.40227199657057	40.0	39.0	41.0	37.0	41.0
15	39.3559747079627	40.0	39.0	41.0	37.0	41.0
16	39.22572071589326	40.0	39.0	41.0	36.0	41.0
17	39.258278855428145	40.0	39.0	41.0	36.0	41.0
18	39.41897974493624	40.0	39.0	41.0	37.0	41.0
19	39.473604115314544	41.0	39.0	41.0	37.0	41.0
20	39.50133962061944	41.0	39.0	41.0	37.0	41.0
21	39.51341764012432	41.0	39.0	41.0	37.0	41.0
22	39.41708284213911	41.0	39.0	41.0	36.0	41.0
23	39.35900760904512	41.0	39.0	41.0	36.0	41.0
24	39.301886185832174	41.0	39.0	41.0	36.0	41.0
25	39.27602614939449	41.0	39.0	41.0	36.0	41.0
26	39.14680098596077	41.0	39.0	41.0	35.0	41.0
27	38.98664666166542	40.0	38.0	41.0	35.0	41.0
28	38.836105454935165	40.0	38.0	41.0	35.0	41.0
29	38.61550744829064	40.0	38.0	41.0	35.0	41.0
30	38.41683635194513	40.0	37.0	41.0	35.0	41.0
31	38.18144893366199	40.0	37.0	41.0	35.0	41.0
32	37.9734219269103	40.0	37.0	41.0	35.0	41.0
33	37.77957346479477	40.0	35.0	41.0	34.0	41.0
34	37.63870967741936	40.0	35.0	41.0	34.0	41.0
35	37.463680205765726	40.0	35.0	41.0	33.0	41.0
36	37.32751044904083	40.0	35.0	41.0	33.0	41.0
37	37.1331797235023	40.0	35.0	41.0	33.0	41.0
38	36.944336084021	40.0	35.0	41.0	33.0	41.0
39	36.67410781266745	40.0	35.0	41.0	32.0	41.0
40	36.44398242417747	40.0	35.0	41.0	31.0	41.0
41	36.154227842674956	40.0	35.0	41.0	30.0	41.0
42	35.87269317329332	40.0	35.0	41.0	27.0	41.0
43	34.86931732933233	38.0	35.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	0.0
14	2.0
15	0.0
16	0.0
17	3.0
18	3.0
19	4.0
20	15.0
21	29.0
22	50.0
23	77.0
24	130.0
25	208.0
26	288.0
27	456.0
28	651.0
29	933.0
30	1144.0
31	1360.0
32	1675.0
33	2061.0
34	2833.0
35	3757.0
36	6042.0
37	13887.0
38	20962.0
39	36737.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.02529203729504	14.411102775693923	18.761118851141354	22.80248633586968
2	20.175758225270606	21.15957560818776	34.816204051012754	23.848462115528882
3	19.226235130211126	23.575179509163004	32.03300825206302	25.165577108562854
4	13.122923588039868	20.729825313471224	31.499303397277888	34.64794770121102
5	13.246168685028401	38.12024434680099	31.116707748365663	17.516879219804952
6	25.887900546565213	31.862608509270174	21.3953488372093	20.854142106955308
7	27.318615368127748	33.062908584288934	18.57035687493302	21.048119172650306
8	32.95359554174258	29.592755331690064	18.032365234165685	19.42128389240167
9	30.380452255921124	13.196870646232986	16.811702925731435	39.61097417211445
10	21.61719001178866	26.904940520844495	22.024434680098594	29.453434787268247
11	31.910834851570037	26.015432429535956	15.349908905797879	26.723823813096132
12	23.596613439074055	32.55385274890151	23.472296645589967	20.377237166434465
13	34.7068910084664	18.164183903118637	26.13760583002894	20.991319258386028
14	23.824884792626726	21.907619762083378	25.93934197835173	28.32815346693816
15	31.88189904619012	23.62126245847176	21.231379273389773	23.265459221948344
16	21.77365770013932	26.46125817168578	27.289679562747832	24.475404565427073
17	17.16000428678598	32.25377773014682	26.3615903975994	24.224627585467793
18	21.976208337798735	22.289143714500053	30.5229878898296	25.21166005787161
19	22.154109956060445	29.532740327939127	30.921658986175117	17.391490729825314
20	25.479584181759723	21.96656306933876	33.066123673775586	19.487729075125923
21	30.634444325367056	20.049298038795413	29.063337262887153	20.25292037295038
22	27.726931732933235	21.760797342192692	25.507448290644092	25.004822634229985
23	23.45514950166113	24.23427285392777	26.458043082199122	25.852534562211982
24	21.152073732718893	25.371342835708926	31.99871396420534	21.47786946736684
25	20.53156146179402	24.39717072125174	28.94116386239417	26.13010395456007
26	26.1429643125067	21.756510556210483	27.917693709141574	24.18283142214125
27	23.94812988961526	22.72425249169435	26.937091415711073	26.390526202979313
28	21.466080805915766	22.818561783302968	32.1369628121316	23.578394598649663
29	21.788661451077054	23.83345836459115	33.458364591147784	20.91951559318401
30	20.91094202121959	24.495766798842567	34.732611724359664	19.860679455578182
31	21.222805701425358	26.28657164291073	30.02572071589326	22.464901939770655
32	19.562747829814597	25.772157325045548	34.7272532418819	19.937841603257958
33	20.818776122602078	23.49480227199657	34.86764548279927	20.818776122602078
34	21.486443039331263	24.51505733576251	29.371985853606258	24.62651377129997
35	19.34197835173079	25.330618368877932	30.83592326653092	24.491480012860357
36	16.891008466402315	24.08423534455042	32.34272853927768	26.682027649769584
37	17.55117350766263	23.795948987246813	34.15711070624799	24.495766798842567
38	18.312078019504877	19.65169863894545	36.19226235130211	25.84396099024756
39	18.347444003858108	19.88211338548923	38.32708177044261	23.443360840210055
40	17.289679562747832	20.459757796592005	35.51495016611296	26.73561247454721
41	13.54624370378309	22.447754795841817	38.71396420533705	25.292037295038046
42	13.571964419676346	22.057657271460723	37.09355910406173	27.2768192048012
43	12.188404243918121	25.58996892080163	37.822312721037406	24.399314114242845
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	16.5
2	30.0
3	116.0
4	202.0
5	202.0
6	269.0
7	336.0
8	363.0
9	390.0
10	576.5
11	763.0
12	763.0
13	1316.5
14	1870.0
15	2833.0
16	3796.0
17	3213.0
18	2630.0
19	2630.0
20	2861.0
21	3092.0
22	2104.0
23	1116.0
24	939.0
25	762.0
26	762.0
27	700.0
28	638.0
29	651.0
30	664.0
31	655.5
32	647.0
33	647.0
34	693.0
35	739.0
36	835.5
37	932.0
38	1293.5
39	1655.0
40	1655.0
41	2480.5
42	3306.0
43	4671.5
44	6037.0
45	7694.5
46	9352.0
47	9352.0
48	11509.0
49	13666.0
50	12289.5
51	10913.0
52	8454.0
53	5995.0
54	5995.0
55	4883.0
56	3771.0
57	3609.5
58	3448.0
59	3310.0
60	3172.0
61	3172.0
62	3000.5
63	2829.0
64	2727.5
65	2626.0
66	2485.0
67	2344.0
68	2344.0
69	2166.0
70	1988.0
71	1725.5
72	1463.0
73	1232.5
74	1002.0
75	1002.0
76	783.5
77	565.0
78	453.0
79	341.0
80	238.5
81	136.0
82	136.0
83	93.0
84	50.0
85	40.0
86	30.0
87	19.0
88	8.0
89	8.0
90	5.0
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	93310.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.7547958418176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.90538308808522	51.347122494909435
2	5.429478020448684	6.2715678919729925
3	1.8667309940435324	3.2343800235773226
4	0.8535748084095675	1.9719215518165256
5	0.5269896643224286	1.5218090236844926
6	0.3878198586034774	1.3439074054227844
7	0.2764840140283164	1.1177794448612153
8	0.23194967619825205	1.0716964955524595
9	0.16885936427232748	0.8777194298574644
>10	1.1671707706296042	13.279391276390525
>50	0.09463546788888683	3.9824241774729394
>100	0.08350188343137073	8.57464366091523
>500	0.001855597409586016	0.6097953059693495
>1k	0.005566792228758049	4.795841817597257
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1798	1.9269102990033222	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1463	1.5678919729932481	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1214	1.3010395456006858	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	569	0.6097953059693495	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	499	0.5347765512806772	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	327	0.3504447540456543	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	326	0.3493730575501018	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	297	0.3182938591790805	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	275	0.29471653627692634	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	260	0.2786410888436395	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	248	0.26578073089701	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	242	0.2593505519236952	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	229	0.2454184974815132	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	226	0.24220340799485587	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	219	0.23470153252598863	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	215	0.2304147465437788	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	211	0.22612796056156897	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	196	0.21005251312828208	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	195	0.2089808166327296	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	191	0.20469403065051978	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	180	0.19290536919944273	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	178	0.1907619762083378	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	177	0.18969027971278532	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	174	0.18647519022612796	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	155	0.16611295681063123	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	150	0.16075447433286894	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	149	0.15968277783731646	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	145	0.15539599185510664	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	141	0.1511092058728968	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT	137	0.14682241989068698	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	132	0.14146393741292465	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	132	0.14146393741292465	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT	130	0.13932054442181974	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	127	0.13610545493516235	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	127	0.13610545493516235	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	126	0.1350337584396099	No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC	125	0.13396206194405744	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTA	125	0.13396206194405744	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT	123	0.13181866895295252	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	120	0.12860357946629514	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	119	0.12753188297074267	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT	119	0.12753188297074267	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	119	0.12753188297074267	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	113	0.12110170399742794	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107	0.11467152502411317	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA	105	0.11252813203300824	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	105	0.11252813203300824	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT	104	0.11145643553745578	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	101	0.10824134605079841	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	100	0.10716964955524595	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	99	0.1060979530596935	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	99	0.1060979530596935	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	96	0.10288286357303612	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0010716964955524595	0.0
2	0.0	0.0	0.0	0.0010716964955524595	0.0
3	0.0	0.0	0.0	0.0010716964955524595	0.0
4	0.0	0.0	0.0	0.0010716964955524595	0.0
5	0.0	0.0	0.0	0.0010716964955524595	0.0
6	0.0	0.0	0.0	0.0010716964955524595	0.0010716964955524595
7	0.0	0.0	0.0	0.0010716964955524595	0.0010716964955524595
8	0.0	0.0	0.0	0.0010716964955524595	0.0010716964955524595
9	0.0	0.0	0.0	0.002143392991104919	0.0010716964955524595
10	0.0	0.0	0.0	0.002143392991104919	0.0010716964955524595
11	0.0	0.0	0.0	0.002143392991104919	0.0010716964955524595
12	0.0	0.0	0.0	0.0032150894866573784	0.0010716964955524595
13	0.0	0.0	0.0	0.0032150894866573784	0.0010716964955524595
14	0.0	0.0	0.0	0.004286785982209838	0.0010716964955524595
15	0.0	0.0	0.0	0.005358482477762298	0.0010716964955524595
16	0.0	0.0	0.0	0.011788661451077055	0.0010716964955524595
17	0.0	0.0	0.0	0.01929053691994427	0.0010716964955524595
18	0.0	0.0	0.0	0.02036223341549673	0.0010716964955524595
19	0.0	0.0	0.0	0.026792412388811488	0.0010716964955524595
20	0.0	0.0	0.0	0.03322259136212625	0.0010716964955524595
21	0.0	0.0	0.0	0.0503697352909656	0.0010716964955524595
22	0.0	0.0	0.0	0.062158396742042654	0.0010716964955524595
23	0.0	0.0	0.0	0.09430929160861644	0.0010716964955524595
24	0.0	0.0	0.0	0.1746865287750509	0.0010716964955524595
25	0.0	0.0	0.0	0.1907619762083378	0.0010716964955524595
26	0.0	0.0	0.0	0.2336298360304362	0.0010716964955524595
27	0.0	0.0	0.0	0.36330511199228377	0.002143392991104919
28	0.0	0.0	0.0	0.7491158503911692	0.002143392991104919
29	0.0	0.0	0.0	1.522880720180045	0.002143392991104919
30	0.0	0.0	0.0	2.8978673239738506	0.002143392991104919
31	0.0	0.0	0.0	5.4463615903976	0.002143392991104919
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACAA	20	0.0018332822	37.0	1
ATATGCT	20	0.0018332822	37.0	35
ATGCCCC	55	4.8748916E-10	33.636364	37
TGTTGAT	25	0.005470817	29.6	2
GTGTTGA	25	0.005470817	29.6	1
TATGCTG	25	0.005470817	29.6	36
ATACAAC	25	0.005470817	29.6	2
TATTGAC	70	2.0190782E-10	29.07143	30
ATTGACT	65	2.91584E-9	28.461538	31
ACCGACC	35	8.808204E-4	26.428572	32
TTATTGC	50	8.974765E-6	25.900002	29
TATTGCT	50	8.974765E-6	25.900002	30
TTGACTG	75	1.3396857E-8	24.666666	32
ATTGCTG	55	1.878485E-5	23.545454	31
GTTGAAC	40	0.0019179898	23.125002	3
TGACTGT	80	2.658453E-8	23.125002	33
TGGGCAG	480	0.0	21.968752	6
GGCAGTC	485	0.0	21.360825	8
CAGTCGG	495	0.0	20.929293	10
AGTCGGT	495	0.0	20.929293	11
>>END_MODULE