FastQCFastQC Report
Fri 10 Feb 2017
ERR1631568.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631568.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656397
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT123861.8869677954043058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100941.537788868626761No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT87001.325417392218429No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33240.5064008519234549No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA31590.48126362551931223No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA20150.3069788557839234No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA14820.2257779971572082No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA14650.22318810110344808No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14150.21557075976885942No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC13480.2053635223805106No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT13220.20140250488652447No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC13130.2000313834462985No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT12890.19637505960569593No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT11720.17855048088275846No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT11140.1697143649346356No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10520.16026886167974563No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA10170.15493672274553358No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10160.1547843759188418No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA10140.15447968226545825No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA9920.15112805207823923No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9700.14777642189102022No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9510.1448818321838765No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA8940.13619806306244545No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT8480.12919010903462386No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG7190.10953736839138509No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA7040.10725216599100848No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6780.10329114849702238No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA6640.10115829292333756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAG303.5978112E-430.8333325
GTCTGAC250.005494646729.61
TATAGAG358.8653265E-426.428572
ATGCCCC3450.026.27536237
ACTGTTA451.322367E-424.66666824
TTAGTCC451.322367E-424.66666828
TATTGAC2750.023.54545630
ATTGACT2750.023.54545631
TTGAATA400.001930309923.1254
TATGCCT903.8216967E-922.61111336
GTTAGTC502.7003908E-422.227
CCGGATA603.7231523E-521.5833324
GGGCAGT26900.020.7695187
AGTCGGT26900.020.63197111
CAGTCGG27000.020.62407310
GATACCG450.003824084820.5555575
AGTCTTA450.003824084820.55555727
GTTATAC450.003824084820.5555573
GCAGTCG27100.020.5479729
TGGGCAG27700.020.3032476
Produced by FastQC (version 0.11.5)