##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631568.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	656397
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.773071784301266	34.0	31.0	34.0	31.0	34.0
2	32.9033024221622	34.0	33.0	34.0	31.0	34.0
3	32.951029636028196	34.0	33.0	34.0	31.0	34.0
4	36.36395656896665	37.0	37.0	37.0	35.0	37.0
5	36.3586076718815	37.0	37.0	37.0	35.0	37.0
6	36.43260404907396	37.0	37.0	37.0	35.0	37.0
7	36.41873744090847	37.0	37.0	37.0	35.0	37.0
8	36.42046048351836	37.0	37.0	37.0	35.0	37.0
9	38.25279061299793	39.0	39.0	39.0	37.0	39.0
10	38.21159146065567	39.0	39.0	39.0	37.0	39.0
11	38.27158868794343	39.0	39.0	39.0	37.0	39.0
12	38.06439090976954	39.0	39.0	39.0	37.0	39.0
13	38.18087681692634	39.0	39.0	39.0	37.0	39.0
14	39.60642873139274	41.0	39.0	41.0	37.0	41.0
15	39.59171507487085	41.0	39.0	41.0	37.0	41.0
16	39.51692801764786	41.0	39.0	41.0	37.0	41.0
17	39.51615104883173	41.0	39.0	41.0	37.0	41.0
18	39.61685382474326	41.0	39.0	41.0	37.0	41.0
19	39.65459622758788	41.0	40.0	41.0	37.0	41.0
20	39.66747258137987	41.0	40.0	41.0	37.0	41.0
21	39.64711752186558	41.0	40.0	41.0	37.0	41.0
22	39.579117515771706	41.0	40.0	41.0	37.0	41.0
23	39.51783143433014	41.0	39.0	41.0	37.0	41.0
24	39.47509205557003	41.0	39.0	41.0	36.0	41.0
25	39.424826743571344	41.0	39.0	41.0	36.0	41.0
26	39.28773745157275	41.0	39.0	41.0	36.0	41.0
27	39.16974026389517	41.0	39.0	41.0	35.0	41.0
28	39.042375269844314	41.0	39.0	41.0	35.0	41.0
29	38.87683825489757	40.0	38.0	41.0	35.0	41.0
30	38.68177642493796	40.0	38.0	41.0	35.0	41.0
31	38.46547440040098	40.0	37.0	41.0	35.0	41.0
32	38.27049179079124	40.0	37.0	41.0	35.0	41.0
33	38.091702125390576	40.0	36.0	41.0	35.0	41.0
34	37.96350227072945	40.0	36.0	41.0	34.0	41.0
35	37.79043779907587	40.0	35.0	41.0	34.0	41.0
36	37.630824028750894	40.0	35.0	41.0	34.0	41.0
37	37.45197951849262	40.0	35.0	41.0	33.0	41.0
38	37.25370012355328	40.0	35.0	41.0	33.0	41.0
39	37.0212660935379	40.0	35.0	41.0	33.0	41.0
40	36.802785509379234	40.0	35.0	41.0	33.0	41.0
41	36.51355658237317	39.0	35.0	41.0	31.0	41.0
42	36.23738225494632	39.0	35.0	41.0	31.0	41.0
43	35.18098041276849	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	10.0
12	3.0
13	5.0
14	5.0
15	1.0
16	6.0
17	9.0
18	26.0
19	51.0
20	82.0
21	149.0
22	264.0
23	440.0
24	701.0
25	1056.0
26	1530.0
27	2374.0
28	3540.0
29	5072.0
30	6374.0
31	7786.0
32	9742.0
33	13095.0
34	17773.0
35	25457.0
36	41454.0
37	96018.0
38	140520.0
39	282852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.1619979981627	14.970817965347191	14.422826429736881	25.444357606753233
2	19.681534193483515	20.43839322848825	33.49344984818639	26.386622729841847
3	20.225564711599837	22.226183239716207	29.546295915429226	28.001956133254723
4	14.183337218177414	17.44066471967422	31.284268514328982	37.091729547819384
5	14.668714208017406	37.48387027972401	30.81001284283749	17.0374026694211
6	31.080276113388695	31.509437124179424	18.947527182482553	18.462759579949328
7	30.88527217522323	30.875826671968337	17.89679111878939	20.342110034019043
8	33.27452745823031	29.046903017533598	17.498251820163713	20.180317704072383
9	30.514764692708834	12.610356232584854	17.2151914161704	39.65968765853592
10	20.6170960561977	26.13844974916095	25.07126022818508	28.173193966456278
11	34.83379722942061	24.691002548762413	16.506016937920194	23.96918328389679
12	24.61696199099021	27.793393327513684	24.6393569745139	22.95028770698221
13	34.55881120724196	18.171777140968043	24.0022425452889	23.267169106501097
14	24.660380836597366	20.371055931090485	24.357362998307426	30.611200234004727
15	31.288381878649655	23.80434401741629	20.637205837321012	24.27006826661304
16	25.007579254627917	24.765500147014688	24.647583703155256	25.579336895202143
17	21.843640357893165	28.194217828539742	25.238080003412566	24.724061810154527
18	23.575062043245172	22.452722971006875	28.005612457095324	25.966602528652626
19	24.030731401880264	26.74341899795398	28.614238029728963	20.611611570436793
20	25.74828952600332	21.943275182549584	30.04888809668539	22.259547194761705
21	27.682027797201997	21.353845310079116	27.76018171929488	23.20394517342401
22	26.966911792710814	21.980447808262376	25.2819558894998	25.77068450952701
23	23.98365623243251	24.024942222465977	26.21995530144105	25.771446243660467
24	23.70303337766626	23.86101703694563	28.710673571024852	23.72527601436326
25	23.22024628388003	23.45760263986581	26.959294451376227	26.362856624877935
26	25.722999952772486	22.80860515815886	26.696496175332918	24.771898713735744
27	24.68765091857519	22.738525617880644	25.760934312618733	26.81288915092543
28	22.742029594894554	23.595171824368485	28.23519912491983	25.427599455817134
29	22.914790896363023	24.036672928121245	29.592609350743526	23.455926824772202
30	22.336177648587668	24.937347367523007	30.710835058661146	22.01563992522818
31	22.730298889239286	25.77510256750107	27.670449438373424	23.82414910488622
32	21.399701704913337	25.320042596172744	30.595356164028782	22.68489953488514
33	22.979690644533722	23.690997978357608	30.22317286642078	23.10613851068789
34	23.74523344865988	24.612696279842837	27.107375566920627	24.53469470457665
35	22.56058452430465	25.80922825668003	27.83148003418663	23.798707184828693
36	20.728613933336078	25.31958555569267	29.0912359441009	24.860564566870355
37	21.615729505162275	25.18216871801669	28.819906245762855	24.382195531058183
38	21.812561605248042	22.72725195270545	30.8487089368172	24.611477505229303
39	22.278438201271484	22.680633823737768	30.687678341004	24.35324963398675
40	21.110242734198966	22.586635831668943	29.76963636335937	26.533485070772716
41	18.66416208483585	23.381276879693235	32.31687530564582	25.637685729825087
42	19.353226781962746	22.579780224467815	31.227747841626336	26.839245151943107
43	18.165987961553757	24.33283515921005	32.82601840044973	24.675158478786464
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21.0
1	66.5
2	112.0
3	503.5
4	895.0
5	895.0
6	1242.5
7	1590.0
8	1515.0
9	1440.0
10	2483.5
11	3527.0
12	3527.0
13	6503.5
14	9480.0
15	15972.0
16	22464.0
17	19666.5
18	16869.0
19	16869.0
20	18528.5
21	20188.0
22	13564.5
23	6941.0
24	6217.0
25	5493.0
26	5493.0
27	5166.0
28	4839.0
29	5563.0
30	6287.0
31	7226.5
32	8166.0
33	8166.0
34	9157.5
35	10149.0
36	11184.5
37	12220.0
38	14166.5
39	16113.0
40	16113.0
41	18872.0
42	21631.0
43	24883.5
44	28136.0
45	33285.0
46	38434.0
47	38434.0
48	50226.5
49	62019.0
50	62280.0
51	62541.0
52	55216.5
53	47892.0
54	47892.0
55	42093.5
56	36295.0
57	35152.5
58	34010.0
59	31906.5
60	29803.0
61	29803.0
62	29843.0
63	29883.0
64	28819.0
65	27755.0
66	26153.5
67	24552.0
68	24552.0
69	22625.0
70	20698.0
71	18673.0
72	16648.0
73	14429.0
74	12210.0
75	12210.0
76	10004.5
77	7799.0
78	6334.0
79	4869.0
80	3653.5
81	2438.0
82	2438.0
83	1830.0
84	1222.0
85	855.0
86	488.0
87	324.0
88	160.0
89	160.0
90	118.0
91	76.0
92	53.5
93	31.0
94	20.5
95	10.0
96	10.0
97	6.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	656397.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.312120632670975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.15780345221759	43.69627483553474
2	8.39460790858482	8.614902673385561
3	2.3084998413831377	3.553620670246601
4	1.0797790103274796	2.216230033381988
5	0.590076583008641	1.5139040404926836
6	0.38984530417281765	1.2002273565477557
7	0.27529083791507974	0.988802967891735
8	0.20384745473443952	0.8367876150397198
9	0.16548325702915748	0.7642167162630682
>10	1.1843866827743155	12.164584326501892
>50	0.14617885852424142	5.214317746939105
>100	0.0919944288068844	8.489592670352797
>500	0.006549765157771611	2.2380028542757713
>1k	0.004763465569288444	3.7453321486879925
>5k	2.9771659808052776E-4	1.329052889569912
>10k+	5.954331961610555E-4	3.4341504548886923
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12386	1.8869677954043058	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10094	1.537788868626761	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8700	1.325417392218429	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3324	0.5064008519234549	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	3159	0.48126362551931223	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	2015	0.3069788557839234	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	1482	0.2257779971572082	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	1465	0.22318810110344808	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1415	0.21557075976885942	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	1348	0.2053635223805106	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	1322	0.20140250488652447	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	1313	0.2000313834462985	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	1289	0.19637505960569593	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	1172	0.17855048088275846	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	1114	0.1697143649346356	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1052	0.16026886167974563	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	1017	0.15493672274553358	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1016	0.1547843759188418	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	1014	0.15447968226545825	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	992	0.15112805207823923	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	970	0.14777642189102022	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	951	0.1448818321838765	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	894	0.13619806306244545	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	848	0.12919010903462386	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	719	0.10953736839138509	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	704	0.10725216599100848	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	678	0.10329114849702238	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	664	0.10115829292333756	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.5234682669177342E-4	0.0
13	0.0	0.0	0.0	3.0469365338354684E-4	0.0
14	0.0	0.0	0.0	9.140809601506406E-4	0.0
15	0.0	0.0	0.0	0.0012187746135341874	0.0
16	0.0	0.0	0.0	0.0027422428804519216	0.0
17	0.0	0.0	0.0	0.005484485760903843	0.0
18	0.0	0.0	0.0	0.006398566721054484	0.0
19	0.0	0.0	0.0	0.008226728641355764	0.0
20	0.0	0.0	0.0	0.011426012001883007	1.5234682669177342E-4
21	0.0	0.0	0.0	0.015387029495869117	1.5234682669177342E-4
22	0.0	0.0	0.0	0.02361375813722488	1.5234682669177342E-4
23	0.0	0.0	0.0	0.03321160821880661	1.5234682669177342E-4
24	0.0	0.0	0.0	0.04981741232820991	1.5234682669177342E-4
25	1.5234682669177342E-4	0.0	0.0	0.056825366356031484	1.5234682669177342E-4
26	1.5234682669177342E-4	0.0	0.0	0.0659661759575379	1.5234682669177342E-4
27	1.5234682669177342E-4	0.0	0.0	0.1043575762838648	1.5234682669177342E-4
28	1.5234682669177342E-4	0.0	0.0	0.24162206713315265	1.5234682669177342E-4
29	1.5234682669177342E-4	0.0	0.0	0.4809589318659287	1.5234682669177342E-4
30	1.5234682669177342E-4	0.0	0.0	0.8021060425321871	1.5234682669177342E-4
31	1.5234682669177342E-4	0.0	0.0	1.549824267935411	1.5234682669177342E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACAG	30	3.5978112E-4	30.833332	5
GTCTGAC	25	0.0054946467	29.6	1
TATAGAG	35	8.8653265E-4	26.42857	2
ATGCCCC	345	0.0	26.275362	37
ACTGTTA	45	1.322367E-4	24.666668	24
TTAGTCC	45	1.322367E-4	24.666668	28
TATTGAC	275	0.0	23.545456	30
ATTGACT	275	0.0	23.545456	31
TTGAATA	40	0.0019303099	23.125	4
TATGCCT	90	3.8216967E-9	22.611113	36
GTTAGTC	50	2.7003908E-4	22.2	27
CCGGATA	60	3.7231523E-5	21.583332	4
GGGCAGT	2690	0.0	20.769518	7
AGTCGGT	2690	0.0	20.631971	11
CAGTCGG	2700	0.0	20.624073	10
GATACCG	45	0.0038240848	20.555557	5
AGTCTTA	45	0.0038240848	20.555557	27
GTTATAC	45	0.0038240848	20.555557	3
GCAGTCG	2710	0.0	20.547972	9
TGGGCAG	2770	0.0	20.303247	6
>>END_MODULE