FastQCFastQC Report
Fri 10 Feb 2017
ERR1631567.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631567.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259343
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16960.6539601994270137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13600.5244020467103411No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10090.38906004789024573No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA6120.2359809210196535No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC5710.22017174167029765No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC5580.21515907504733112No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.21245994686573377No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5250.2024346136198008No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA5050.19472281881523695No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT4920.18971015219227047No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT4460.17197302414177362No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC4240.16349004985675342No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA4140.1596341524544715No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC4120.1588629729740151No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC3760.1449817423258002No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG3720.14343938336488743No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT3670.14151143466374647No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG3640.14035466544306188No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG3600.13881230648214912No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG3560.13726994752123636No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG3510.1353419988200954No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG3470.13379963985918264No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA3450.13302846037872626No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA3280.12647343479484696No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC3260.12570225531439058No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC3180.12261753739256506No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT2990.11529133232822941No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC2980.11490574258800122No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG2870.11066425544549112No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG2840.10950748622480654No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG2810.10835071700412197No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC2790.10757953752366556No Hit
CCCCCACTCCGAGAAGGCCAGCGTGTCCTCTTTGTGTCTTTTC2770.10680835804320918No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG2750.1060371785627528No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC2730.1052659990822964No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA2700.10410922986161184No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC2700.10410922986161184No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT2690.10372364012138365No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG2660.10256687090069908No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA2630.10141010168001449No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC2600.10025333245932992No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG2600.10025333245932992No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATCTC250.005488594529.59999812
ATACACA652.9667717E-928.46153837
TATTGAC358.8508055E-426.42857230
ATTGACT358.8508055E-426.42857231
TTGACTT400.001927177923.1250024
GGGCAGT4350.022.1149437
AGTCGGT4150.021.84337411
GGCAGTC4300.021.0813948
CAGTCGG4300.021.08139410
GCAGTCG4450.020.7865169
GCCTTAT3300.020.74242626
CGCCTTA3350.020.43283725
TGGGCAG4900.020.0102046
TCGCCTT3600.019.52777724
TCGGTGA4650.019.49462313
GTGATTC4600.019.30434816
CGGTGAT4600.019.30434814
GATTCCT4650.019.09677318
TTATACA2250.018.91111237
GTCGGTG4800.018.88541812
Produced by FastQC (version 0.11.5)