##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631567.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	259343
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.807575296036525	34.0	31.0	34.0	31.0	34.0
2	32.966615640291046	34.0	33.0	34.0	31.0	34.0
3	33.05731791488492	34.0	33.0	34.0	31.0	34.0
4	36.402308911364486	37.0	37.0	37.0	35.0	37.0
5	36.37932005105208	37.0	37.0	37.0	35.0	37.0
6	36.434359130572254	37.0	37.0	37.0	35.0	37.0
7	36.43368820442426	37.0	37.0	37.0	35.0	37.0
8	36.43029501471025	37.0	37.0	37.0	35.0	37.0
9	38.25444681367918	39.0	39.0	39.0	37.0	39.0
10	38.23311984514716	39.0	39.0	39.0	37.0	39.0
11	38.27089607199731	39.0	39.0	39.0	37.0	39.0
12	38.067262274285405	39.0	39.0	39.0	37.0	39.0
13	38.193107197803684	39.0	39.0	39.0	37.0	39.0
14	39.686931978113925	41.0	40.0	41.0	37.0	41.0
15	39.68051576483653	41.0	40.0	41.0	37.0	41.0
16	39.61629193770412	41.0	40.0	41.0	37.0	41.0
17	39.62169404996472	41.0	40.0	41.0	37.0	41.0
18	39.6209884207401	41.0	40.0	41.0	37.0	41.0
19	39.61637676744697	41.0	40.0	41.0	37.0	41.0
20	39.6072922731672	41.0	40.0	41.0	37.0	41.0
21	39.578785623672125	41.0	40.0	41.0	37.0	41.0
22	39.50146716896157	41.0	39.0	41.0	37.0	41.0
23	39.43250444392176	41.0	39.0	41.0	36.0	41.0
24	39.37847560952098	41.0	39.0	41.0	36.0	41.0
25	39.35943518814852	41.0	39.0	41.0	36.0	41.0
26	39.26569446640164	41.0	39.0	41.0	36.0	41.0
27	39.17315292874687	41.0	39.0	41.0	35.0	41.0
28	39.043197618597766	41.0	39.0	41.0	35.0	41.0
29	38.95736148652556	40.0	39.0	41.0	35.0	41.0
30	38.88029752104356	40.0	38.0	41.0	35.0	41.0
31	38.78631002186294	40.0	38.0	41.0	35.0	41.0
32	38.69754726366241	40.0	38.0	41.0	35.0	41.0
33	38.53566897891981	40.0	38.0	41.0	35.0	41.0
34	38.48641374550306	40.0	38.0	41.0	35.0	41.0
35	38.32784767662902	40.0	38.0	41.0	35.0	41.0
36	38.2499161342315	40.0	37.0	41.0	34.0	41.0
37	38.133564430117644	40.0	37.0	41.0	34.0	41.0
38	37.98961992419306	40.0	37.0	41.0	34.0	41.0
39	37.851918116162764	40.0	37.0	41.0	34.0	41.0
40	37.67510594078113	40.0	36.0	41.0	33.0	41.0
41	37.51591136063052	40.0	36.0	41.0	33.0	41.0
42	37.33020748583922	40.0	35.0	41.0	33.0	41.0
43	36.36569330963242	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	9.0
19	10.0
20	28.0
21	54.0
22	93.0
23	153.0
24	260.0
25	359.0
26	547.0
27	762.0
28	1182.0
29	1562.0
30	1926.0
31	2483.0
32	3186.0
33	4417.0
34	6170.0
35	9265.0
36	14724.0
37	28776.0
38	57855.0
39	125517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.06772883787108	16.721484674735777	12.174996047705163	26.03579043968798
2	22.05419078209167	19.091319218178242	29.61252087004469	29.241969129685398
3	22.98384764578184	20.11120408108181	26.741805254045804	30.163143019090548
4	15.987321809341298	14.797391870997096	30.525982964645276	38.68930335501633
5	17.769131998935773	33.68126380893257	30.304268864014066	18.24533532811759
6	36.553907373632605	29.98384378988444	15.536952992754768	17.92529584372819
7	32.134277771137064	28.180440574837185	18.278496045777214	21.406785608248533
8	32.170523206718514	26.461867102640134	18.401499172910007	22.966110517731344
9	28.213986882237034	13.365697165529818	18.60123465834821	39.819081293884935
10	22.118969858450008	23.327022514584932	26.621501255094604	27.93250637187046
11	38.478385767111504	21.14304222593245	17.399351438056936	22.979220568899102
12	26.42870638498051	24.5219651195521	22.962640210069292	26.0866882853981
13	33.34772868363519	16.386021600737248	23.428818206005175	26.83743150962239
14	25.57539628985552	19.798876391496975	21.062068380484533	33.56365893816297
15	30.31043829985772	24.493431478775214	18.834516451186268	26.361613770180803
16	28.618084929996186	22.732828724893288	22.086580320270837	26.562506024839692
17	27.27237673659979	24.23354399386141	21.52708960719973	26.966989662339063
18	27.017501918308955	20.00940838966157	25.017833525485557	27.95525616654392
19	27.55000134956409	23.17779928511662	24.02339758543705	25.24880177988224
20	28.57335652012971	20.25695700288807	23.278823797056408	27.890862679925814
21	30.075999737798973	20.922870484262155	23.546808666515002	25.454321111423866
22	29.844260303921832	20.377261001839265	22.616380623344373	27.16209807089453
23	26.992438585194122	20.76786340869042	23.221756515502637	29.01794149061282
24	26.451841769394203	22.456360881149674	24.633400554478047	26.45839679497808
25	26.92071889351168	21.886073655352178	22.939890415395826	28.25331703574031
26	27.99998457641039	22.71894749424507	23.209417643815332	26.071650285529202
27	28.517060418056396	20.058378286670546	23.250290156279522	28.174271138993532
28	26.034633670467294	21.965890731579414	24.10090112322291	27.898574474730374
29	25.842609979833657	22.413174830244117	25.13659516547584	26.607620024446387
30	25.565756546349817	22.69388416113024	24.9268343467917	26.813524945728247
31	26.024993926961592	23.637807845208854	23.601176819887176	26.736021407942378
32	25.727704237245657	21.953166270151883	23.758883023640507	28.560246468961953
33	24.821568347709405	21.959335705995535	24.80113209147731	28.417963854817753
34	27.997285448228794	21.294193404101904	23.107621952395093	27.600899195274213
35	26.68049648534952	21.586084837454646	24.154112507374403	27.579306169821432
36	24.008359585568147	23.480487231195752	25.19751834443189	27.31363483880421
37	26.29953382200406	21.253320891637713	25.654827776342525	26.792317510015696
38	25.57578187959575	20.46787459079289	25.98142228631581	27.974921243295558
39	26.227042950841163	19.919180390448172	26.223187053438885	27.630589605271783
40	24.507312709423427	20.504120026374338	26.320741257716612	28.66782600648562
41	22.56625395711471	21.588398375896016	28.283007445737884	27.56234022125139
42	24.303335736842712	21.28609601955711	27.874282321096	26.53628592250417
43	23.328564873545847	21.176202943592077	27.82685478304793	27.668377399814148
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	27.0
1	32.5
2	38.0
3	113.5
4	189.0
5	189.0
6	251.0
7	313.0
8	282.5
9	252.0
10	402.5
11	553.0
12	553.0
13	1005.5
14	1458.0
15	2122.5
16	2787.0
17	2424.0
18	2061.0
19	2061.0
20	2524.5
21	2988.0
22	2000.5
23	1013.0
24	897.5
25	782.0
26	782.0
27	828.0
28	874.0
29	1024.0
30	1174.0
31	1408.5
32	1643.0
33	1643.0
34	2169.0
35	2695.0
36	3666.5
37	4638.0
38	5608.0
39	6578.0
40	6578.0
41	7363.5
42	8149.0
43	8911.0
44	9673.0
45	11624.0
46	13575.0
47	13575.0
48	17016.5
49	20458.0
50	20843.5
51	21229.0
52	20700.0
53	20171.0
54	20171.0
55	18900.5
56	17630.0
57	18107.0
58	18584.0
59	19306.5
60	20029.0
61	20029.0
62	19223.5
63	18418.0
64	17999.5
65	17581.0
66	15746.0
67	13911.0
68	13911.0
69	12722.5
70	11534.0
71	9941.5
72	8349.0
73	5758.0
74	3167.0
75	3167.0
76	2507.0
77	1847.0
78	1611.0
79	1375.0
80	1276.0
81	1177.0
82	1177.0
83	1027.5
84	878.0
85	757.0
86	636.0
87	590.5
88	545.0
89	545.0
90	411.5
91	278.0
92	168.0
93	58.0
94	39.5
95	21.0
96	21.0
97	13.5
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	259343.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.81010476473242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.45384458011651	29.16870707904204
2	7.769917412324623	5.564831130973267
3	2.851266810952827	3.0631248963727575
4	1.6291414973457807	2.3335891078610183
5	0.9981587363116581	1.78720844595767
6	0.689127930139656	1.4806646024762573
7	0.531920621076547	1.3333693217090878
8	0.3994788470028319	1.1444303489972738
9	0.2918026079185106	0.9404533764165603
>10	2.5896135499779263	18.986053219095947
>50	0.4673148776259543	11.991069741616316
>100	0.3187216676895909	19.35814731841615
>500	0.0064605743450592765	1.280929117038054
>1k	0.0032302871725296382	1.5674222940276004
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1696	0.6539601994270137	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1360	0.5244020467103411	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1009	0.38906004789024573	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	612	0.2359809210196535	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	571	0.22017174167029765	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	558	0.21515907504733112	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	551	0.21245994686573377	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	525	0.2024346136198008	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	505	0.19472281881523695	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	492	0.18971015219227047	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	446	0.17197302414177362	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	424	0.16349004985675342	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	414	0.1596341524544715	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	412	0.1588629729740151	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	376	0.1449817423258002	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	372	0.14343938336488743	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	367	0.14151143466374647	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	364	0.14035466544306188	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	360	0.13881230648214912	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	356	0.13726994752123636	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	351	0.1353419988200954	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	347	0.13379963985918264	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	345	0.13302846037872626	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	328	0.12647343479484696	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	326	0.12570225531439058	No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC	318	0.12261753739256506	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	299	0.11529133232822941	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	298	0.11490574258800122	No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG	287	0.11066425544549112	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	284	0.10950748622480654	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	281	0.10835071700412197	No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC	279	0.10757953752366556	No Hit
CCCCCACTCCGAGAAGGCCAGCGTGTCCTCTTTGTGTCTTTTC	277	0.10680835804320918	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	275	0.1060371785627528	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	273	0.1052659990822964	No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA	270	0.10410922986161184	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	270	0.10410922986161184	No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT	269	0.10372364012138365	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG	266	0.10256687090069908	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA	263	0.10141010168001449	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	260	0.10025333245932992	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG	260	0.10025333245932992	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	3.85589740228192E-4	0.0
15	0.0	0.0	0.0	0.001542358960912768	0.0
16	0.0	0.0	0.0	0.003470307662053728	0.0
17	0.0	0.0	0.0	0.006940615324107456	0.0
18	0.0	0.0	0.0	0.008097384544792032	0.0
19	0.0	0.0	0.0	0.0096397435057048	0.0
20	0.0	0.0	0.0	0.01156769220684576	0.0
21	0.0	0.0	0.0	0.01542358960912768	0.0
22	0.0	0.0	0.0	0.0192794870114096	0.0
23	0.0	0.0	0.0	0.025448922855060674	0.0
24	0.0	0.0	0.0	0.033931897140080894	0.0
25	0.0	0.0	0.0	0.03624543558145005	0.0
26	0.0	0.0	0.0	0.05128343545034954	0.0
27	0.0	0.0	0.0	0.0917703581743097	0.0
28	0.0	0.0	0.0	0.24369271582421734	0.0
29	0.0	0.0	0.0	0.43186050905557505	0.0
30	0.0	0.0	0.0	0.6574305070890674	0.0
31	0.0	0.0	3.85589740228192E-4	1.1494430156202404	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCATCTC	25	0.0054885945	29.599998	12
ATACACA	65	2.9667717E-9	28.461538	37
TATTGAC	35	8.8508055E-4	26.428572	30
ATTGACT	35	8.8508055E-4	26.428572	31
TTGACTT	40	0.0019271779	23.125002	4
GGGCAGT	435	0.0	22.114943	7
AGTCGGT	415	0.0	21.843374	11
GGCAGTC	430	0.0	21.081394	8
CAGTCGG	430	0.0	21.081394	10
GCAGTCG	445	0.0	20.786516	9
GCCTTAT	330	0.0	20.742426	26
CGCCTTA	335	0.0	20.432837	25
TGGGCAG	490	0.0	20.010204	6
TCGCCTT	360	0.0	19.527777	24
TCGGTGA	465	0.0	19.494623	13
GTGATTC	460	0.0	19.304348	16
CGGTGAT	460	0.0	19.304348	14
GATTCCT	465	0.0	19.096773	18
TTATACA	225	0.0	18.911112	37
GTCGGTG	480	0.0	18.885418	12
>>END_MODULE