FastQCFastQC Report
Fri 10 Feb 2017
ERR1631566.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631566.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800478
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25290.31593622810370803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20430.25522250455352924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18040.2253653442068364No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA16890.21099892814043608No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC13700.17114773922581258No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC13590.16977356029772211No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC13530.1690240081551273No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT11960.14941072709056338No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG11780.14716207066277898No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC9760.12192714852875407No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA9140.11418177638860781No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG9050.11305744817471561No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT9040.11293252281761648No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC8780.10968446353303901No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC8540.10668625496265982No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA8300.10368804639228062No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT8280.10343819567808234No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC8270.10331327032098321No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8230.10281356889258668No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT8100.10118953925029794No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG8070.10081476317900055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACA454.0062587E-628.7777774
GTTATAC454.0062587E-628.7777773
ACTGATC1750.024.3142878
GGTATCA12450.023.032131
ATAACAC603.7244667E-521.58333434
CTAGGCA450.003824808420.5555554
AACACGC450.003824808420.55555536
AGTCGGT1750.020.08571411
ATACACA2800.019.8214337
CTAGACA2900.019.7758624
TCTAGAC3000.019.7333343
ACATAAC759.260011E-619.73333432
GTCTAGA2650.019.5471691
GCCTCTA2400.019.27083425
GTTCTAG3450.018.7681161
TAGAACT3750.018.7466684
GCAGTCG1900.018.59
GCGTTTC701.2186104E-418.514
GACGAAG500.007033061718.57
GACATGT2550.018.1372557
Produced by FastQC (version 0.11.5)