##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631566.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	800478
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.800746553934026	34.0	31.0	34.0	31.0	34.0
2	32.96511834179078	34.0	33.0	34.0	31.0	34.0
3	33.07971487036496	34.0	33.0	34.0	31.0	34.0
4	36.417568003118134	37.0	37.0	37.0	35.0	37.0
5	36.39001696486349	37.0	37.0	37.0	35.0	37.0
6	36.42662759001497	37.0	37.0	37.0	35.0	37.0
7	36.428404028592915	37.0	37.0	37.0	35.0	37.0
8	36.41772790757523	37.0	37.0	37.0	35.0	37.0
9	38.25889156229153	39.0	39.0	39.0	37.0	39.0
10	38.23281214474351	39.0	39.0	39.0	37.0	39.0
11	38.27861477767034	39.0	39.0	39.0	37.0	39.0
12	38.06722608241576	39.0	39.0	39.0	37.0	39.0
13	38.19131693812947	39.0	39.0	39.0	37.0	39.0
14	39.69654881208478	41.0	40.0	41.0	37.0	41.0
15	39.6873792908737	41.0	40.0	41.0	37.0	41.0
16	39.65998316006186	41.0	40.0	41.0	37.0	41.0
17	39.66701645766654	41.0	40.0	41.0	37.0	41.0
18	39.64972553899045	41.0	40.0	41.0	37.0	41.0
19	39.64001384172957	41.0	40.0	41.0	37.0	41.0
20	39.60670999078051	41.0	40.0	41.0	37.0	41.0
21	39.581338900007246	41.0	40.0	41.0	37.0	41.0
22	39.50867981381125	41.0	39.0	41.0	37.0	41.0
23	39.438956473507076	41.0	39.0	41.0	37.0	41.0
24	39.390942661759595	41.0	39.0	41.0	36.0	41.0
25	39.392689118251845	41.0	39.0	41.0	36.0	41.0
26	39.321616584091004	41.0	39.0	41.0	36.0	41.0
27	39.244302779089494	41.0	39.0	41.0	36.0	41.0
28	39.129652532611765	41.0	39.0	41.0	35.0	41.0
29	39.056766082265845	41.0	39.0	41.0	35.0	41.0
30	39.011195810503224	40.0	39.0	41.0	35.0	41.0
31	38.96142055122065	40.0	39.0	41.0	35.0	41.0
32	38.873129555090834	40.0	38.0	41.0	35.0	41.0
33	38.75815575193822	40.0	38.0	41.0	35.0	41.0
34	38.71692663633479	40.0	38.0	41.0	35.0	41.0
35	38.60533331334528	40.0	38.0	41.0	35.0	41.0
36	38.54630858062308	40.0	38.0	41.0	35.0	41.0
37	38.474086733176925	40.0	38.0	41.0	35.0	41.0
38	38.3772695814251	40.0	38.0	41.0	35.0	41.0
39	38.28817781375628	40.0	38.0	41.0	34.0	41.0
40	38.17985003960134	40.0	37.0	41.0	34.0	41.0
41	38.07640309914826	40.0	37.0	41.0	34.0	41.0
42	37.92621033932225	40.0	37.0	41.0	34.0	41.0
43	36.99242702485265	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	3.0
14	1.0
15	5.0
16	7.0
17	13.0
18	18.0
19	41.0
20	71.0
21	140.0
22	284.0
23	394.0
24	661.0
25	999.0
26	1374.0
27	2027.0
28	2936.0
29	4128.0
30	5526.0
31	7136.0
32	9351.0
33	12499.0
34	18038.0
35	26438.0
36	41984.0
37	78079.0
38	167660.0
39	420664.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.573215004035085	18.210244378983557	12.151864261103992	26.064676355877364
2	20.045647725484024	20.273261726118644	32.53356119718468	27.14752935121265
3	20.90650836125415	21.018066705143674	28.370298746498968	29.705126187103204
4	15.085611347220034	15.183677752542856	32.278088841916954	37.45262205832016
5	16.848807837317203	34.61219421395716	31.08505168162023	17.453946267105405
6	35.40809366403574	33.12758127019106	15.379810563188496	16.084514502584707
7	30.80259544921909	29.492753080034678	19.460622278188783	20.24402919255745
8	28.8703749509668	30.20920000299821	19.62215076491796	21.29827428111703
9	27.473209757170093	13.90344269299094	19.45525048783352	39.16809706200545
10	19.78792671378851	24.462258800366783	29.334472652590076	26.41534183325463
11	38.22153763126532	20.834926131636347	19.662501655260982	21.281034581837353
12	24.279992704359145	25.180329752972597	25.553481794627707	24.986195748040547
13	31.103040933042507	17.53839580850442	24.931353516274026	26.42720974217905
14	24.535090283555576	19.958699676943027	22.689068281701683	32.81714175779972
15	27.462716027173762	25.812327134537117	20.516366471033557	26.208590367255564
16	26.876441326307532	24.487243871786607	22.613738291370904	26.022576510534957
17	26.19372424976077	24.81242457631565	22.834481397365074	26.159369776558506
18	26.30603214579289	22.130527010111457	25.002810820534734	26.560630023560922
19	25.803582359540172	23.948940508046444	24.165810927970536	26.081666204442843
20	26.446198396458115	22.49331024712734	24.11634048655928	26.94415086985526
21	28.48697903002956	23.261851044001208	23.98042169803542	24.270748227933808
22	28.43451038004792	22.594000084949244	23.118436734051404	25.85305280095143
23	26.1898515636907	22.82473721951134	24.19691734188822	26.78849387490974
24	25.437301212525515	24.071117507289394	24.04013601872881	26.45144526145628
25	26.053058297667143	22.78326200095443	24.032140795874465	27.13153890550396
26	26.26842961330605	24.145822870834678	23.915585437700972	25.6701620781583
27	27.044840707677164	22.449086670714248	24.090106161568464	26.415966460040124
28	25.413440469319582	23.00825256908997	25.270400935441074	26.307906026149375
29	24.866392330582478	23.46235624214532	25.108497672640596	26.562753754631608
30	24.155192272617114	24.457261786082814	25.73175027920817	25.6557956620919
31	26.25618692831033	23.62975622065816	23.359167897181436	26.754888953850077
32	25.495516428933712	22.961405560177795	24.1283333208408	27.414744690047698
33	24.6758811610063	22.58413098173841	25.310002273641498	27.429985583613792
34	26.315776323646624	22.430472792506478	25.119366178708226	26.13438470513868
35	25.58783626782997	22.235089534003432	26.27142782187643	25.905646376290164
36	24.16106376440077	22.600746054232594	26.328768560784933	26.909421620581703
37	25.21580855438875	21.684168709196257	26.56800061962977	26.53202211678522
38	23.831760523087457	20.638043768848114	27.945552532361912	27.584643175702517
39	24.56344833961708	20.724117339889418	27.925189699154757	26.78724462133875
40	23.35704416611075	21.62270543350348	28.465366943251407	26.55488345713436
41	22.83947841164904	21.760623027740923	29.334972354018475	26.06492620659156
42	23.52406936855229	21.93177076696674	29.188809686212487	25.35535017826848
43	21.45730426070423	21.070285504411114	29.34134354723053	28.131066687654126
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	197.0
1	174.0
2	151.0
3	324.5
4	498.0
5	498.0
6	605.5
7	713.0
8	681.0
9	649.0
10	935.0
11	1221.0
12	1221.0
13	1966.0
14	2711.0
15	3957.0
16	5203.0
17	4669.5
18	4136.0
19	4136.0
20	4637.0
21	5138.0
22	4225.5
23	3313.0
24	3633.5
25	3954.0
26	3954.0
27	4497.5
28	5041.0
29	6130.5
30	7220.0
31	8571.0
32	9922.0
33	9922.0
34	13075.5
35	16229.0
36	20086.0
37	23943.0
38	27451.0
39	30959.0
40	30959.0
41	34246.5
42	37534.0
43	41291.5
44	45049.0
45	50068.5
46	55088.0
47	55088.0
48	58388.5
49	61689.0
50	62496.0
51	63303.0
52	64472.5
53	65642.0
54	65642.0
55	62920.5
56	60199.0
57	59115.0
58	58031.0
59	55853.5
60	53676.0
61	53676.0
62	51116.0
63	48556.0
64	45351.5
65	42147.0
66	37230.0
67	32313.0
68	32313.0
69	26489.0
70	20665.0
71	17457.0
72	14249.0
73	10434.0
74	6619.0
75	6619.0
76	5147.5
77	3676.0
78	3254.0
79	2832.0
80	2682.5
81	2533.0
82	2533.0
83	2169.5
84	1806.0
85	1627.0
86	1448.0
87	1345.0
88	1242.0
89	1242.0
90	965.5
91	689.0
92	435.0
93	181.0
94	126.5
95	72.0
96	72.0
97	52.0
98	32.0
99	20.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	800478.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.55107633082847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.86510580149273	36.95966700584644
2	6.993514586041797	6.091501751149371
3	2.4481142636641398	3.1985403349028068
4	1.29707481298523	2.2595601674845924
5	0.7822250372066784	1.7033371151636594
6	0.5332505263384271	1.3934180625611579
7	0.4006332998122573	1.2213607994556714
8	0.28891134571131893	1.006592005593282
9	0.22582857367353715	0.8851569704764504
>10	1.7131430384052435	15.062711552735045
>50	0.2458416410270221	7.578764254976219
>100	0.1876235404198984	16.022846006292507
>500	0.016139659390957368	4.793900367542709
>1k	0.002593873830689577	1.8226436058201159
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2529	0.31593622810370803	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2043	0.25522250455352924	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1804	0.2253653442068364	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1689	0.21099892814043608	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1370	0.17114773922581258	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1359	0.16977356029772211	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1353	0.1690240081551273	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1196	0.14941072709056338	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1178	0.14716207066277898	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	976	0.12192714852875407	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	914	0.11418177638860781	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	905	0.11305744817471561	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	904	0.11293252281761648	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	878	0.10968446353303901	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	854	0.10668625496265982	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	830	0.10368804639228062	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	828	0.10343819567808234	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	827	0.10331327032098321	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	823	0.10281356889258668	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	810	0.10118953925029794	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG	807	0.10081476317900055	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.2492535709913326E-4	0.0
11	0.0	0.0	0.0	3.747760712973998E-4	0.0
12	0.0	0.0	0.0	7.495521425947996E-4	0.0
13	0.0	0.0	0.0	9.994028567930661E-4	0.0
14	0.0	0.0	0.0	0.001873880356486999	0.0
15	0.0	0.0	0.0	0.002248656427784399	0.0
16	0.0	0.0	0.0	0.004497312855568798	0.0
17	0.0	0.0	0.0	0.0061213424978575305	0.0
18	0.0	0.0	0.0	0.00687089464045233	0.0
19	0.0	0.0	0.0	0.007495521425947996	0.0
20	0.0	0.0	0.0	0.008619849639840195	1.2492535709913326E-4
21	0.0	0.0	0.0	0.010868506067624595	1.2492535709913326E-4
22	0.0	0.0	0.0	0.013491938566706392	1.2492535709913326E-4
23	0.0	0.0	0.0	0.018488952850671724	1.2492535709913326E-4
24	0.0	0.0	0.0	0.02748357856180932	1.2492535709913326E-4
25	0.0	0.0	0.0	0.031980891417378116	1.2492535709913326E-4
26	0.0	0.0	0.0	0.047221784983472376	1.2492535709913326E-4
27	0.0	0.0	0.0	0.09969043496510835	1.2492535709913326E-4
28	0.0	0.0	0.0	0.29882145418112677	1.2492535709913326E-4
29	0.0	0.0	0.0	0.5587911223044231	1.2492535709913326E-4
30	0.0	0.0	0.0	0.9298194328888489	1.2492535709913326E-4
31	0.0	0.0	0.0	1.8177888711494883	1.2492535709913326E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGACA	45	4.0062587E-6	28.777777	4
GTTATAC	45	4.0062587E-6	28.777777	3
ACTGATC	175	0.0	24.314287	8
GGTATCA	1245	0.0	23.03213	1
ATAACAC	60	3.7244667E-5	21.583334	34
CTAGGCA	45	0.0038248084	20.555555	4
AACACGC	45	0.0038248084	20.555555	36
AGTCGGT	175	0.0	20.085714	11
ATACACA	280	0.0	19.82143	37
CTAGACA	290	0.0	19.775862	4
TCTAGAC	300	0.0	19.733334	3
ACATAAC	75	9.260011E-6	19.733334	32
GTCTAGA	265	0.0	19.547169	1
GCCTCTA	240	0.0	19.270834	25
GTTCTAG	345	0.0	18.768116	1
TAGAACT	375	0.0	18.746668	4
GCAGTCG	190	0.0	18.5	9
GCGTTTC	70	1.2186104E-4	18.5	14
GACGAAG	50	0.0070330617	18.5	7
GACATGT	255	0.0	18.137255	7
>>END_MODULE