FastQCFastQC Report
Fri 10 Feb 2017
ERR1631565.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631565.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328916
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC29280.22032995313473538No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT24850.1869945128209759No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA23970.1803725743387844No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC19040.1432746689783252No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC18550.13958745323255947No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC17820.13409425426437788No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC14330.10783224823841388No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT14160.10655301012253596No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTCG551.902949E-523.54545410
AACGTTA400.001931344723.1250028
ATACACA7450.023.0939637
GGTATCA15450.021.4336571
CAGGACA11350.020.0484584
TGCATCA9950.019.70854214
TATACAC15000.019.48666637
TATACCA1052.2602762E-819.380955
ATACCAT1355.638867E-1119.1851846
GACAGGC10550.018.5876797
ATCTATT1002.8757677E-718.58
ACGTTAT500.007035445418.59
TAAGTAG500.007035445418.55
TCGTATC500.007035445418.526
GTATATA500.007035445418.54
CTTAGGT801.6169797E-518.54
GCATCAG10650.018.41314515
TCCAGGA11600.018.1810342
CCAGGAC12150.018.1193433
ACAGGCT10950.017.7397258
Produced by FastQC (version 0.11.5)