##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631565.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1328916
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.784620698373715	34.0	31.0	34.0	31.0	34.0
2	32.96599634589395	34.0	33.0	34.0	31.0	34.0
3	33.07020383530637	34.0	33.0	34.0	31.0	34.0
4	36.423101234389534	37.0	37.0	37.0	35.0	37.0
5	36.408578871802284	37.0	37.0	37.0	35.0	37.0
6	36.448930556935125	37.0	37.0	37.0	35.0	37.0
7	36.43686056906532	37.0	37.0	37.0	35.0	37.0
8	36.41642285893164	37.0	37.0	37.0	35.0	37.0
9	38.26626965135494	39.0	39.0	39.0	37.0	39.0
10	38.231077058294126	39.0	39.0	39.0	37.0	39.0
11	38.286100852123084	39.0	39.0	39.0	37.0	39.0
12	38.06255549635944	39.0	39.0	39.0	37.0	39.0
13	38.18089630947328	39.0	39.0	39.0	37.0	39.0
14	39.691172354008835	41.0	40.0	41.0	37.0	41.0
15	39.696530856728344	41.0	40.0	41.0	37.0	41.0
16	39.68034021713938	41.0	40.0	41.0	37.0	41.0
17	39.66495850753547	41.0	40.0	41.0	37.0	41.0
18	39.60712490480964	41.0	40.0	41.0	37.0	41.0
19	39.619212952511674	41.0	40.0	41.0	37.0	41.0
20	39.600624870194956	41.0	40.0	41.0	37.0	41.0
21	39.569595820954824	41.0	40.0	41.0	37.0	41.0
22	39.51615527241752	41.0	39.0	41.0	37.0	41.0
23	39.446622660875484	41.0	39.0	41.0	37.0	41.0
24	39.400293923769446	41.0	39.0	41.0	36.0	41.0
25	39.38084273197102	41.0	39.0	41.0	36.0	41.0
26	39.30948532488133	41.0	39.0	41.0	36.0	41.0
27	39.239097881280685	41.0	39.0	41.0	36.0	41.0
28	39.13117006643008	41.0	39.0	41.0	35.0	41.0
29	39.04354225549245	40.0	39.0	41.0	35.0	41.0
30	38.99749118830686	40.0	39.0	41.0	35.0	41.0
31	38.976718618784034	40.0	39.0	41.0	35.0	41.0
32	38.90840880838217	40.0	39.0	41.0	35.0	41.0
33	38.80436837241782	40.0	38.0	41.0	35.0	41.0
34	38.75172621896343	40.0	38.0	41.0	35.0	41.0
35	38.63457208732531	40.0	38.0	41.0	35.0	41.0
36	38.59961577706943	40.0	38.0	41.0	35.0	41.0
37	38.54336240966322	40.0	38.0	41.0	35.0	41.0
38	38.469551122870065	40.0	38.0	41.0	35.0	41.0
39	38.396203371770675	40.0	38.0	41.0	34.0	41.0
40	38.28650268339007	40.0	38.0	41.0	34.0	41.0
41	38.20344551499117	40.0	37.0	41.0	34.0	41.0
42	38.105822339410466	40.0	37.0	41.0	34.0	41.0
43	37.12477161837167	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	0.0
12	0.0
13	1.0
14	2.0
15	4.0
16	7.0
17	12.0
18	33.0
19	53.0
20	99.0
21	202.0
22	384.0
23	596.0
24	1006.0
25	1452.0
26	2188.0
27	3170.0
28	4410.0
29	5959.0
30	8101.0
31	10790.0
32	14273.0
33	19648.0
34	30863.0
35	47329.0
36	70505.0
37	125998.0
38	292025.0
39	689801.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.56662422606094	16.18349090536949	11.957189167712631	27.29269570085694
2	21.51640886256167	18.465425956192867	31.667840555761238	28.35032462548423
3	20.930066309683983	20.023914227836823	28.004027342585985	31.04199211989321
4	15.674655132453818	14.810943656333434	31.46842990828615	38.045971302926596
5	17.702774291226834	33.04716024188135	31.1477926370064	18.10227282988541
6	36.9345391281315	31.875829623542796	15.189748637235159	15.999882611090543
7	32.07320853989267	28.02502189754657	19.13574672891289	20.766022833647877
8	29.589755861168047	30.282576174867337	18.500416881127173	21.62725108283744
9	28.255510506307395	13.96198104319611	17.68403721529427	40.098471235202226
10	20.584672018396947	22.804601645250717	28.469895764668347	28.14083057168399
11	39.10623395308658	20.03459962856945	19.080664240629204	21.77850217771477
12	26.051608980552572	23.03644474142835	24.536539555547527	26.37540672247155
13	31.581153361085274	16.473877957673775	23.606006700197753	28.338961981043198
14	24.92422395395947	18.670931797043604	21.596097872250766	34.80874637674616
15	28.357322810471093	24.033422729502842	19.67603670961897	27.933217750407096
16	28.286136971787535	22.932826454042242	20.605516074755663	28.175520499414556
17	27.248750109111487	23.484855325694024	21.818309057908852	27.448085507285636
18	27.09155431946037	20.91193122815889	23.581701175996077	28.414813276384663
19	27.712586800068628	22.40006140342956	22.25159453268679	27.63575726381502
20	27.939087195879953	21.38306710130663	22.168970800261263	28.508874902552154
21	31.122960367698184	21.618296416026293	21.343937464820954	25.914805751454566
22	30.33306845579404	21.575931059600457	20.847743574462193	27.243256910143305
23	28.048048183632375	21.656146814396095	21.825683489400383	28.47012151257115
24	27.55682074713526	22.484265371174704	22.073855683880698	27.885058197809343
25	27.3944327557197	21.25386405160296	22.481255399137343	28.87044779354
26	28.168296566524898	22.70730429914306	22.790454776675126	26.333944357656918
27	29.328038792519617	21.237911199804955	21.749832194058918	27.684217813616513
28	27.176209783011117	21.549217557768888	23.338420186076473	27.936152473143522
29	26.51559617011158	21.602494062830157	24.18843628942687	27.693473477631393
30	25.71246038124306	22.3324122818899	24.218837007004204	27.736290329862832
31	27.07477372535209	21.890849384009222	22.450026939249735	28.584349951388955
32	25.88583477059498	20.98033284270789	23.8237029277998	29.31012945889733
33	26.401367731293778	20.778062721797312	24.24133654798347	28.57923299892544
34	27.292244205051336	20.570675648423226	24.802621083650134	27.334459062875304
35	26.972434676081857	19.874393866880975	25.635028850581975	27.518142606455186
36	25.08668719467596	20.634938551420856	25.265329035093266	29.013045218809918
37	24.7644696880766	19.263068546093205	26.661354066020728	29.31110769980947
38	24.25450517564692	18.323656273233222	28.476592952451472	28.94524559866839
39	23.842966748838904	18.287912855289573	29.43338781382721	28.435732582044317
40	23.361596970764143	18.913987039060405	30.057806512977493	27.666609477197955
41	21.57284583826216	19.139810191163324	31.598987445406635	27.68835652516788
42	21.752014423786004	20.175993065024425	30.605771922378842	27.46622058881073
43	20.02669845197138	18.80081209045568	31.202724626688216	29.96976483088472
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	30.5
2	31.0
3	102.0
4	173.0
5	173.0
6	244.5
7	316.0
8	339.5
9	363.0
10	464.0
11	565.0
12	565.0
13	961.5
14	1358.0
15	1958.5
16	2559.0
17	2348.5
18	2138.0
19	2138.0
20	2211.5
21	2285.0
22	2049.0
23	1813.0
24	2234.5
25	2656.0
26	2656.0
27	3542.0
28	4428.0
29	5853.5
30	7279.0
31	9574.5
32	11870.0
33	11870.0
34	16289.0
35	20708.0
36	27289.0
37	33870.0
38	41228.0
39	48586.0
40	48586.0
41	54026.5
42	59467.0
43	65597.5
44	71728.0
45	76283.0
46	80838.0
47	80838.0
48	86031.5
49	91225.0
50	97001.5
51	102778.0
52	109517.5
53	116257.0
54	116257.0
55	113184.0
56	110111.0
57	106255.5
58	102400.0
59	98522.0
60	94644.0
61	94644.0
62	90320.0
63	85996.0
64	79537.5
65	73079.0
66	64819.0
67	56559.0
68	56559.0
69	48291.0
70	40023.0
71	35810.5
72	31598.0
73	28724.5
74	25851.0
75	25851.0
76	23547.5
77	21244.0
78	17133.5
79	13023.0
80	9057.5
81	5092.0
82	5092.0
83	3718.5
84	2345.0
85	2022.5
86	1700.0
87	1440.5
88	1181.0
89	1181.0
90	875.0
91	569.0
92	344.0
93	119.0
94	80.5
95	42.0
96	42.0
97	29.0
98	16.0
99	9.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1328916.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.194012082300524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.34658888784786	37.29655603687137
2	6.5345155226039315	5.645038848706691
3	2.0211265664610836	2.6190169599473583
4	1.0746745774436899	1.8567802673057583
5	0.647081059622586	1.3975013553782902
6	0.48689014437227746	1.261844326726152
7	0.3431846907016825	1.0376466573640328
8	0.269615901512499	0.9316634006008984
9	0.21662002486504867	0.8421019174160237
>10	1.6126938664242363	14.048175279913078
>50	0.22054294236054744	6.688904568117769
>100	0.20352148402687326	18.118956768010964
>500	0.018565642491070112	5.577688109202658
>1k	0.004378689266761819	2.678125504438999
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2928	0.22032995313473538	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	2485	0.1869945128209759	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2397	0.1803725743387844	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1904	0.1432746689783252	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1855	0.13958745323255947	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1782	0.13409425426437788	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1433	0.10783224823841388	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1416	0.10655301012253596	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	7.524930093399433E-5	0.0	0.0	0.0	0.0
3	7.524930093399433E-5	0.0	0.0	0.0	0.0
4	7.524930093399433E-5	0.0	0.0	0.0	0.0
5	7.524930093399433E-5	1.5049860186798866E-4	0.0	0.0	0.0
6	7.524930093399433E-5	1.5049860186798866E-4	0.0	0.0	0.0
7	7.524930093399433E-5	1.5049860186798866E-4	0.0	0.0	0.0
8	7.524930093399433E-5	1.5049860186798866E-4	0.0	7.524930093399433E-5	0.0
9	7.524930093399433E-5	1.5049860186798866E-4	0.0	3.762465046699716E-4	0.0
10	7.524930093399433E-5	1.5049860186798866E-4	0.0	5.267451065379602E-4	0.0
11	1.5049860186798866E-4	1.5049860186798866E-4	0.0	7.524930093399432E-4	0.0
12	1.5049860186798866E-4	1.5049860186798866E-4	0.0	9.782409121419262E-4	0.0
13	1.5049860186798866E-4	1.5049860186798866E-4	0.0	9.782409121419262E-4	0.0
14	1.5049860186798866E-4	1.5049860186798866E-4	0.0	0.0011287395140099148	0.0
15	2.2574790280198296E-4	1.5049860186798866E-4	0.0	0.0012792381158779034	0.0
16	2.2574790280198296E-4	1.5049860186798866E-4	0.0	0.001655484620547875	0.0
17	2.2574790280198296E-4	1.5049860186798866E-4	0.0	0.002106980426151841	0.0
18	2.2574790280198296E-4	1.5049860186798866E-4	0.0	0.0022574790280198297	0.0
19	2.2574790280198296E-4	1.5049860186798866E-4	0.0	0.0027089748336237957	0.0
20	2.2574790280198296E-4	1.5049860186798866E-4	0.0	0.003009972037359773	0.0
21	2.2574790280198296E-4	1.5049860186798866E-4	0.0	0.004364459454171671	0.0
22	2.2574790280198296E-4	1.5049860186798866E-4	0.0	0.006471439880323512	0.0
23	2.2574790280198296E-4	1.5049860186798866E-4	0.0	0.010309154227957222	0.0
24	2.2574790280198296E-4	1.5049860186798866E-4	0.0	0.01881232523349858	0.0
25	2.2574790280198296E-4	1.5049860186798866E-4	0.0	0.02505801721102011	0.0
26	2.2574790280198296E-4	1.5049860186798866E-4	0.0	0.0423653564258388	0.0
27	2.2574790280198296E-4	1.5049860186798866E-4	0.0	0.14244692666805125	0.0
28	2.2574790280198296E-4	1.5049860186798866E-4	0.0	0.5794196171917563	0.0
29	2.2574790280198296E-4	2.2574790280198296E-4	0.0	1.1844239967010706	0.0
30	2.2574790280198296E-4	2.2574790280198296E-4	0.0	2.0327093661299886	0.0
31	2.2574790280198296E-4	2.2574790280198296E-4	0.0	3.804378907319951	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCTCG	55	1.902949E-5	23.545454	10
AACGTTA	40	0.0019313447	23.125002	8
ATACACA	745	0.0	23.09396	37
GGTATCA	1545	0.0	21.433657	1
CAGGACA	1135	0.0	20.048458	4
TGCATCA	995	0.0	19.708542	14
TATACAC	1500	0.0	19.486666	37
TATACCA	105	2.2602762E-8	19.38095	5
ATACCAT	135	5.638867E-11	19.185184	6
GACAGGC	1055	0.0	18.587679	7
ATCTATT	100	2.8757677E-7	18.5	8
ACGTTAT	50	0.0070354454	18.5	9
TAAGTAG	50	0.0070354454	18.5	5
TCGTATC	50	0.0070354454	18.5	26
GTATATA	50	0.0070354454	18.5	4
CTTAGGT	80	1.6169797E-5	18.5	4
GCATCAG	1065	0.0	18.413145	15
TCCAGGA	1160	0.0	18.181034	2
CCAGGAC	1215	0.0	18.119343	3
ACAGGCT	1095	0.0	17.739725	8
>>END_MODULE