FastQCFastQC Report
Fri 10 Feb 2017
ERR1631564.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631564.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614756
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81750.3126486754404617No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC69170.2645371116846084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62490.23898979484127775No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59800.22870202802861914No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT56980.21791708289415915No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA40370.1543929911624641No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC36620.14005130880280992No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC34200.1307961431200464No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC33250.12716291692226733No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG32390.12387389110111995No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG30400.11626323832893011No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT28090.10742876199538312No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT27460.10501935935896121No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC27070.10352782439355718No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA451.3238423E-424.6666682
GACAGGC18800.021.9441477
TGCATCA18800.021.84574314
GCATCAG19000.021.71315815
GTTATAC806.9671114E-720.81253
ACAGGCT20500.020.395128
GGTATCA46900.020.3144991
CCAGGAC22600.020.3008843
CAGGACA23850.019.7798754
TCCAGGA22900.019.711792
GCTGCAT20850.019.60911212
ATACACA8650.019.46242737
ATCAGAA21500.019.0162817
CTGCATC21950.018.79498913
CATCAGA22250.018.45842716
TATACAC15450.018.44012837
GCAGTCG21300.018.0657279
AGTCGGT21150.018.01891311
CAGTCGG21600.017.81481410
AAGACGG8900.017.668545
Produced by FastQC (version 0.11.5)