FastQCFastQC Report
Fri 10 Feb 2017
ERR1631563.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631563.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88789
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12711.4314836297289077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11411.2850690963970761No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9601.081215015373526No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.4809154287130163No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT2000.22525312820281793No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.2038540810235502No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.15880345538298665No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC1350.15204586153690208No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1250.1407832051267612No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.13402561128067667No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1160.1306468143576344No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATA1000.11262656410140896No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA920.10361643897329625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC200.001832800237.031
CTCGTAT200.001832800237.029
TATGCCG200.001832800237.033
TCTCGTA200.001832800237.028
GCCGTCT200.001832800237.036
ACTAGCA250.005469394429.62
GTATGCC250.005469394429.632
TCGTATG250.005469394429.630
ACCGTTA250.005469394429.68
AGGTAGC400.001917255623.1250027
ATCTCCG400.001917255623.12500210
TACACAT603.676665E-521.5833345
GGTATCA5800.020.0948281
AGACTAA500.00698509818.525
ACACATC500.00698509818.56
ACTAAGG500.00698509818.527
CGAGACT500.00698509818.523
ACGAGAC500.00698509818.522
GGGATAG500.00698509818.520
ATACACA1001.07542335E-414.837