##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631563.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	88789
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.750689837705124	34.0	31.0	34.0	31.0	34.0
2	32.905179695683024	34.0	33.0	34.0	31.0	34.0
3	32.96769870141571	34.0	33.0	34.0	31.0	34.0
4	36.34467107411954	37.0	37.0	37.0	35.0	37.0
5	36.351631395781006	37.0	37.0	37.0	35.0	37.0
6	36.43924359999549	37.0	37.0	37.0	35.0	37.0
7	36.42686594060075	37.0	37.0	37.0	35.0	37.0
8	36.43968284359549	37.0	37.0	37.0	35.0	37.0
9	38.25161900685896	39.0	39.0	39.0	37.0	39.0
10	38.213123247249094	39.0	39.0	39.0	37.0	39.0
11	38.287648244715	39.0	39.0	39.0	37.0	39.0
12	38.09070943472727	39.0	39.0	39.0	37.0	39.0
13	38.22063543907466	39.0	39.0	39.0	37.0	39.0
14	39.658437419049655	41.0	39.0	41.0	37.0	41.0
15	39.634470486208876	41.0	39.0	41.0	37.0	41.0
16	39.61222673979885	41.0	39.0	41.0	37.0	41.0
17	39.65351563819843	41.0	39.0	41.0	37.0	41.0
18	39.700413339490254	41.0	40.0	41.0	38.0	41.0
19	39.734212571377086	41.0	40.0	41.0	38.0	41.0
20	39.70621360754147	41.0	40.0	41.0	38.0	41.0
21	39.68171732984942	41.0	40.0	41.0	37.0	41.0
22	39.621293178209015	41.0	40.0	41.0	37.0	41.0
23	39.56448433927626	41.0	40.0	41.0	37.0	41.0
24	39.523161652907454	41.0	39.0	41.0	37.0	41.0
25	39.482379574046334	41.0	39.0	41.0	36.0	41.0
26	39.345617137257996	41.0	39.0	41.0	36.0	41.0
27	39.24209079953598	41.0	39.0	41.0	35.0	41.0
28	39.14150401513701	41.0	39.0	41.0	35.0	41.0
29	39.01361655159986	40.0	39.0	41.0	35.0	41.0
30	38.83753618128372	40.0	38.0	41.0	35.0	41.0
31	38.640507270044715	40.0	38.0	41.0	35.0	41.0
32	38.46609377287727	40.0	38.0	41.0	35.0	41.0
33	38.309937041750665	40.0	37.0	41.0	35.0	41.0
34	38.18139634414173	40.0	37.0	41.0	35.0	41.0
35	38.03294326999966	40.0	37.0	41.0	35.0	41.0
36	37.88069468064738	40.0	37.0	41.0	35.0	41.0
37	37.717296061449055	40.0	37.0	41.0	34.0	41.0
38	37.547342576220025	40.0	36.0	41.0	33.0	41.0
39	37.35522418317584	40.0	36.0	41.0	33.0	41.0
40	37.13257272860377	40.0	36.0	41.0	33.0	41.0
41	36.8977125544831	40.0	35.0	41.0	32.0	41.0
42	36.62289247541925	40.0	35.0	41.0	31.0	41.0
43	35.7240086046695	39.0	35.0	41.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	1.0
13	0.0
14	1.0
15	2.0
16	1.0
17	1.0
18	4.0
19	3.0
20	10.0
21	23.0
22	30.0
23	55.0
24	91.0
25	153.0
26	225.0
27	337.0
28	497.0
29	693.0
30	893.0
31	1073.0
32	1236.0
33	1586.0
34	2172.0
35	3046.0
36	4710.0
37	10897.0
38	18470.0
39	42577.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.083039565711964	20.82803049927356	14.263028077802431	21.825901857212042
2	18.893106128011354	22.77872258950996	33.88032301298584	24.44784826949284
3	19.31320321210961	25.250875671535887	31.071416504296707	24.3645046120578
4	13.23024248499251	18.932525425446848	35.038124091948326	32.79910799761232
5	11.990224014235999	39.518408811902376	33.408417709400936	15.082949464460688
6	27.17228485510593	37.59587336269132	18.433589746477605	16.79825203572515
7	26.628298550496122	33.92987870119046	20.893353906452376	18.54846884186104
8	28.571106781245426	32.662829855049615	19.55985538749169	19.206207976213268
9	27.833402786381196	14.335109078827331	21.894604061313903	35.93688407347757
10	19.654461701336878	28.24786854227438	29.16802757098289	22.92964218540585
11	31.48588226018989	25.624795864352567	20.683868497223756	22.20545337823379
12	21.70764396490556	31.80799423351992	25.81513475768395	20.669227043890572
13	29.422563605852076	22.024124610030523	26.58099539357353	21.972316390543874
14	22.3338476613094	22.920632060277736	28.432576107400692	26.312944171012177
15	26.114721418193692	27.61152845510142	25.238486749484736	21.03526337722015
16	22.320332473617228	27.648695221254886	26.92338014844181	23.107592156686078
17	19.57224430954285	31.02974467557918	26.971809571005416	22.42620144387255
18	19.590264559799074	27.40091678023179	30.189550507382673	22.81926815258647
19	21.762830981315254	28.076676164840244	31.49151358839496	18.668979265449547
20	22.29893342643796	26.174413497167443	32.030994830440704	19.49565824595389
21	22.788858980279088	26.262262217166544	30.69637004583901	20.252508756715358
22	22.509545101307594	27.334467107411953	29.27502280687923	20.88096498440122
23	21.133248487988375	27.004471274594827	30.22446474225411	21.637815495162688
24	20.351620133124598	28.69049093919292	29.37413418328847	21.583754744394014
25	20.323463492099247	27.228598137156627	30.59838493507079	21.84955343567334
26	20.424827399790516	27.871695818175674	30.743673202761602	20.959803579272208
27	20.590388449019585	28.41906091970852	29.206320602777367	21.78423002849452
28	19.598148419286172	27.843539177150323	31.814751827366	20.7435605761975
29	20.692878622351866	28.089065086891395	31.236977553525776	19.981078737230966
30	20.222099584407978	27.304057935104574	32.1424951289011	20.331347351586345
31	19.843674329027245	28.100327743301534	31.63117052788071	20.424827399790516
32	19.514804761851128	27.771458176125417	31.815878093007015	20.897858969016433
33	18.940409284933946	27.31194179459167	32.76532002838189	20.98232889209249
34	20.184932818254513	26.406424219216344	31.43970536890831	21.968937593620833
35	19.82903287569406	26.941400398698036	31.23585128788476	21.99371543772314
36	19.48890065210781	26.67222291049567	31.13336111455248	22.705515322844047
37	18.514680872630617	25.995337260246203	32.10758089402967	23.382400973093514
38	18.915631440831636	24.658459944362477	33.87469168478077	22.551216930025117
39	17.91212875468808	25.58988162948113	34.15175303247024	22.34623658336055
40	17.595648109563122	25.424320580252058	34.594375429388776	22.385655880796044
41	16.790368176238047	25.405174064354817	34.389395082724214	23.415062676682922
42	16.408564123934273	26.362499859216793	33.93663629503655	23.292299721812388
43	15.78010789624841	26.253252092038426	34.322945409904385	23.643694601808782
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	16.5
2	23.0
3	81.0
4	139.0
5	139.0
6	193.0
7	247.0
8	281.0
9	315.0
10	489.0
11	663.0
12	663.0
13	1153.5
14	1644.0
15	2215.5
16	2787.0
17	2524.0
18	2261.0
19	2261.0
20	2343.0
21	2425.0
22	1770.5
23	1116.0
24	976.0
25	836.0
26	836.0
27	915.0
28	994.0
29	1203.5
30	1413.0
31	1852.0
32	2291.0
33	2291.0
34	2975.5
35	3660.0
36	4230.0
37	4800.0
38	5398.5
39	5997.0
40	5997.0
41	6491.5
42	6986.0
43	7394.5
44	7803.0
45	7629.0
46	7455.0
47	7455.0
48	7355.5
49	7256.0
50	7013.5
51	6771.0
52	5715.0
53	4659.0
54	4659.0
55	4047.5
56	3436.0
57	2972.0
58	2508.0
59	2348.0
60	2188.0
61	2188.0
62	2090.0
63	1992.0
64	1800.5
65	1609.0
66	1475.5
67	1342.0
68	1342.0
69	1221.5
70	1101.0
71	967.5
72	834.0
73	693.0
74	552.0
75	552.0
76	450.5
77	349.0
78	265.0
79	181.0
80	135.5
81	90.0
82	90.0
83	62.5
84	35.0
85	24.0
86	13.0
87	8.0
88	3.0
89	3.0
90	2.0
91	1.0
92	1.5
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	88789.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.31215578506347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.50967019340388	47.0260955749023
2	7.668153363067261	8.636204935296039
3	2.926058521170423	4.943179898410839
4	1.5620312406248125	3.5184538625280157
5	0.9080181603632073	2.5566230051019834
6	0.6760135202704054	2.2840667199765736
7	0.5120102402048041	2.0182680286972485
8	0.3140062801256025	1.4145896451136963
9	0.24600492009840194	1.2467760646025972
>10	1.5400308006160122	16.50767550034351
>50	0.11600232004640093	4.423971437903344
>100	0.01600032000640013	1.6263275856243455
>500	0.002000040000800016	1.081215015373526
>1k	0.004000080001600032	2.7165527261259843
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1271	1.4314836297289077	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1141	1.2850690963970761	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	960	1.081215015373526	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	427	0.4809154287130163	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	200	0.22525312820281793	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	181	0.2038540810235502	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	141	0.15880345538298665	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	135	0.15204586153690208	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	125	0.1407832051267612	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	119	0.13402561128067667	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	116	0.1306468143576344	No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATA	100	0.11262656410140896	No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA	92	0.10361643897329625	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0011262656410140897	0.0
12	0.0	0.0	0.0	0.006757593846084538	0.0
13	0.0	0.0	0.0	0.007883859487098627	0.0
14	0.0	0.0	0.0	0.013515187692169075	0.0
15	0.0	0.0	0.0	0.016893984615211344	0.0
16	0.0	0.0	0.0	0.020272781538253612	0.0
17	0.0	0.0	0.0	0.02477784410230997	0.0
18	0.0	0.0	0.0	0.02590410974332406	0.0
19	0.0	0.0	0.0	0.02703037538433815	0.0
20	0.0	0.0	0.0	0.03604050051245087	0.0
21	0.0	0.0	0.0	0.042798094358535405	0.0
22	0.0	0.0	0.0	0.06307087589678902	0.0
23	0.0	0.0	0.0	0.08109112615301445	0.0
24	0.0	0.0	0.0	0.10924776717836669	0.0
25	0.0	0.0	0.0	0.13177307999864848	0.0
26	0.0	0.0	0.0	0.16668731487008526	0.0
27	0.0	0.0	0.0	0.2838189415355506	0.0
28	0.0	0.0	0.0	0.6228248994807916	0.0
29	0.0	0.0	0.0	1.2298820799873857	0.0
30	0.0	0.0	0.0	2.1027379517733054	0.0
31	0.0	0.0	0.0	3.3281149691966347	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	20	0.0018328002	37.0	31
CTCGTAT	20	0.0018328002	37.0	29
TATGCCG	20	0.0018328002	37.0	33
TCTCGTA	20	0.0018328002	37.0	28
GCCGTCT	20	0.0018328002	37.0	36
ACTAGCA	25	0.0054693944	29.6	2
GTATGCC	25	0.0054693944	29.6	32
TCGTATG	25	0.0054693944	29.6	30
ACCGTTA	25	0.0054693944	29.6	8
AGGTAGC	40	0.0019172556	23.125002	7
ATCTCCG	40	0.0019172556	23.125002	10
TACACAT	60	3.676665E-5	21.583334	5
GGTATCA	580	0.0	20.094828	1
AGACTAA	50	0.006985098	18.5	25
ACACATC	50	0.006985098	18.5	6
ACTAAGG	50	0.006985098	18.5	27
CGAGACT	50	0.006985098	18.5	23
ACGAGAC	50	0.006985098	18.5	22
GGGATAG	50	0.006985098	18.5	20
ATACACA	100	1.07542335E-4	14.8	37
>>END_MODULE