FastQCFastQC Report
Fri 10 Feb 2017
ERR1631562.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631562.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945582
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13260.14023109576958953No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA13200.13959656592447825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12780.1351548570086994No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC11480.121406710364622No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC10690.11305206740399035No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC10490.11093696792028612No Hit
GTGTAAGAGCAATTGCTGCCAGCATTCAAGTGCGCTGGGCCTG10410.11009092812680445No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10340.10935064330750796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGT454.00727E-628.77777714
GAATAGA358.868225E-426.4285721
GCACTAT2200.024.38636613
GGCACTA2150.024.09302312
ACTATTC2250.023.84444415
CTATTCA2300.022.52173816
CTTACAC2300.022.5217383
ACTGATC1400.022.4642878
CACTATT2400.021.58333214
TTACACT2650.020.9433964
TCTTACA2750.020.8545442
GGTATCA8100.020.5555551
CTCTTAC2900.020.4137941
AACCGCT656.900475E-519.9230777
GTCTAGA2050.019.8536571
ACATGTA1403.6379788E-1219.821438
ACGGAGT1600.019.6562526
TATTCAT2650.019.5471717
CTTATAC11550.019.38095337
AACGGAG1650.019.06060625
Produced by FastQC (version 0.11.5)