##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631562.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	945582
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.067823837594204	34.0	33.0	34.0	31.0	34.0
2	33.208629182873615	34.0	33.0	34.0	31.0	34.0
3	33.294260043021126	34.0	34.0	34.0	31.0	34.0
4	36.50962899039956	37.0	37.0	37.0	35.0	37.0
5	36.49471753903945	37.0	37.0	37.0	35.0	37.0
6	36.54441074385934	37.0	37.0	37.0	35.0	37.0
7	36.55861046424319	37.0	37.0	37.0	35.0	37.0
8	36.55212768432563	37.0	37.0	37.0	35.0	37.0
9	38.409396540966306	39.0	39.0	39.0	37.0	39.0
10	38.371187268793186	39.0	39.0	39.0	37.0	39.0
11	38.42625917160014	39.0	39.0	39.0	37.0	39.0
12	38.3921405018285	39.0	39.0	39.0	37.0	39.0
13	38.414340586009466	39.0	39.0	39.0	37.0	39.0
14	39.97084864136585	41.0	40.0	41.0	38.0	41.0
15	39.96194618763894	41.0	40.0	41.0	38.0	41.0
16	39.94376267737753	41.0	40.0	41.0	38.0	41.0
17	39.93749563760731	41.0	40.0	41.0	38.0	41.0
18	39.914006400291036	41.0	40.0	41.0	38.0	41.0
19	39.92079058188502	41.0	40.0	41.0	38.0	41.0
20	39.88655135144281	41.0	40.0	41.0	38.0	41.0
21	39.85301539157894	41.0	40.0	41.0	38.0	41.0
22	39.808342375383624	41.0	40.0	41.0	38.0	41.0
23	39.75668635824286	41.0	40.0	41.0	38.0	41.0
24	39.729857378841814	41.0	40.0	41.0	38.0	41.0
25	39.71954203865979	41.0	40.0	41.0	37.0	41.0
26	39.65098108889551	41.0	40.0	41.0	37.0	41.0
27	39.59192328111153	41.0	40.0	41.0	37.0	41.0
28	39.533767563257335	41.0	40.0	41.0	37.0	41.0
29	39.51005518294553	41.0	40.0	41.0	37.0	41.0
30	39.44842118399039	41.0	39.0	41.0	37.0	41.0
31	39.41093104564173	41.0	39.0	41.0	36.0	41.0
32	39.34052255647845	41.0	39.0	41.0	36.0	41.0
33	39.28394258773962	41.0	39.0	41.0	36.0	41.0
34	39.23236059908078	41.0	39.0	41.0	35.0	41.0
35	39.15513197163229	41.0	39.0	41.0	35.0	41.0
36	39.09730409419807	41.0	39.0	41.0	35.0	41.0
37	39.04734227174375	41.0	39.0	41.0	35.0	41.0
38	38.96832850033101	41.0	39.0	41.0	35.0	41.0
39	38.89778887499973	40.0	39.0	41.0	35.0	41.0
40	38.821200065145064	40.0	38.0	41.0	35.0	41.0
41	38.74171991429617	40.0	38.0	41.0	35.0	41.0
42	38.61751598486435	40.0	38.0	41.0	35.0	41.0
43	37.80431839861588	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	0.0
15	0.0
16	2.0
17	2.0
18	7.0
19	26.0
20	52.0
21	76.0
22	141.0
23	261.0
24	411.0
25	730.0
26	1010.0
27	1624.0
28	2257.0
29	3042.0
30	4340.0
31	5648.0
32	7592.0
33	9908.0
34	14798.0
35	21882.0
36	37250.0
37	72177.0
38	182838.0
39	579506.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.17416998208511	16.61431795444499	11.391820064256722	27.81969199921318
2	20.490555023255517	18.63614155091785	32.7257710066393	28.14753241918734
3	21.587339860530342	19.482392854347903	27.151637827285207	31.778629457836548
4	16.124778178941646	14.132565975240647	30.63922536596509	39.10343047985262
5	17.667849007278058	33.38684535027105	31.14706075200247	17.798244890448423
6	37.98359105820543	30.845130300703694	14.538665076111853	16.632613564979028
7	32.584693871076226	27.324441455103845	18.87239816324761	21.218466510572327
8	29.13560114299976	30.585501839079	18.505216892876554	21.77368012504468
9	28.216273152407723	13.424853687993215	18.146390265466135	40.212482894132926
10	19.237781599057513	24.395768954992796	29.980054611868667	26.386394834081024
11	38.95262388666451	19.896423578283002	18.88847291932376	22.262479615728726
12	24.773737232730742	23.09202163323752	25.983256872486997	26.150984261544743
13	32.62773614556961	17.21310261828165	23.020742780636688	27.138418455512053
14	24.97646951824379	18.478037864510956	21.81376126026088	34.731731356984376
15	27.70780323652523	25.282312903587425	19.538231480717698	27.471652379169655
16	27.811231601278365	23.27444896370701	21.354678917322875	27.559640517691747
17	26.880587828448512	23.97835407188377	22.28870684932666	26.852351250341062
18	27.53203846942941	21.87182074108856	23.56876505686445	27.027375732617582
19	27.091145982051266	22.86898439268091	23.00392773974124	27.035941885526587
20	27.199121810694365	22.309223314318587	23.210995979195882	27.280658895791166
21	28.240491041496135	22.36770581504301	23.43318717995901	25.958615963501842
22	28.552468215342508	21.984026768699067	22.154715297034	27.30878971892443
23	26.94689619726264	22.146360654073362	23.131679748556973	27.775063400107026
24	26.93367682548949	22.739751814226583	22.70263181828757	27.623939541996357
25	27.542825476796303	22.403450996317613	22.734781330439876	27.318942196446212
26	27.5256931709783	22.750010046722547	22.514282209263715	27.21001457303544
27	28.021472489958565	21.914228485736825	22.954117146900003	27.110181877404603
28	26.54999777914554	22.62320983267448	23.335469583811875	27.491322804368103
29	26.648667170060342	22.573928014704173	23.11475895268734	27.662645862548146
30	25.764449830897796	22.778140869855815	24.535154010968906	26.922255288277487
31	27.50792633531518	22.341055561548337	22.86676353822302	27.284254564913464
32	26.723541691783474	22.003485683949144	23.486381931974172	27.78659069229321
33	26.614825578321078	22.1361024215774	23.141197696233643	28.10787430386788
34	27.363359285603998	21.742482407660045	23.476123699478205	27.418034607257752
35	26.265834163509883	21.776218244425127	24.376415794716905	27.581531797348084
36	25.76540162566546	21.553921288687814	25.050180735250883	27.630496350395838
37	26.87530007973925	20.770594194897956	25.141658787921088	27.212446937441705
38	25.533057947380556	20.15710958964955	26.301579344784482	28.00825311818541
39	26.175307905607344	20.370311617606934	26.269641342580545	27.184739134205177
40	25.225310972501592	21.119691364683337	25.995207184569924	27.659790478245146
41	24.83317152822283	20.958732293973448	27.367800994519776	26.840295183283946
42	25.027866435697803	20.94096545831033	27.07824387520067	26.952924230791197
43	23.43699435902968	20.81628034374597	26.941396938605006	28.80532835861935
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	158.0
1	130.5
2	103.0
3	222.5
4	342.0
5	342.0
6	376.5
7	411.0
8	370.0
9	329.0
10	505.0
11	681.0
12	681.0
13	1097.5
14	1514.0
15	2081.0
16	2648.0
17	2452.0
18	2256.0
19	2256.0
20	2651.5
21	3047.0
22	2505.0
23	1963.0
24	2205.0
25	2447.0
26	2447.0
27	2991.0
28	3535.0
29	4514.5
30	5494.0
31	6887.0
32	8280.0
33	8280.0
34	10920.5
35	13561.0
36	17055.0
37	20549.0
38	24336.0
39	28123.0
40	28123.0
41	32157.0
42	36191.0
43	39547.5
44	42904.0
45	50466.0
46	58028.0
47	58028.0
48	63347.0
49	68666.0
50	71548.0
51	74430.0
52	78208.5
53	81987.0
54	81987.0
55	83615.5
56	85244.0
57	82950.0
58	80656.0
59	78405.5
60	76155.0
61	76155.0
62	73162.5
63	70170.0
64	63980.5
65	57791.0
66	51755.0
67	45719.0
68	45719.0
69	37770.5
70	29822.0
71	24893.5
72	19965.0
73	14448.0
74	8931.0
75	8931.0
76	6654.0
77	4377.0
78	3551.5
79	2726.0
80	2480.5
81	2235.0
82	2235.0
83	1870.5
84	1506.0
85	1354.5
86	1203.0
87	1038.0
88	873.0
89	873.0
90	660.5
91	448.0
92	260.5
93	73.0
94	50.5
95	28.0
96	28.0
97	18.5
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	945582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.974069758123534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.06845307211607	31.9854161497394
2	8.057523702659312	6.280689817303557
3	2.8465008701436956	3.3281917043861915
4	1.5237232128221536	2.375427791544109
5	0.9343692881994847	1.820808690906748
6	0.6797069926365001	1.589456864765959
7	0.48105328155578153	1.3124022906910489
8	0.35713336800537465	1.1135152638076062
9	0.2584478856272461	0.9065489330948239
>10	2.164468073524092	17.493812790000433
>50	0.36212238478880504	9.973172420096077
>100	0.25427319508110546	18.41958519779808
>500	0.010051397668761971	2.4200242502533276
>1k	0.0021732751716242098	0.9809478356125948
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1326	0.14023109576958953	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1320	0.13959656592447825	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1278	0.1351548570086994	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1148	0.121406710364622	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1069	0.11305206740399035	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1049	0.11093696792028612	No Hit
GTGTAAGAGCAATTGCTGCCAGCATTCAAGTGCGCTGGGCCTG	1041	0.11009092812680445	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1034	0.10935064330750796	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.115099483704216E-4	0.0
10	0.0	0.0	0.0	2.115099483704216E-4	0.0
11	0.0	0.0	0.0	2.115099483704216E-4	0.0
12	0.0	0.0	0.0	4.230198967408432E-4	0.0
13	0.0	0.0	0.0	4.230198967408432E-4	0.0
14	0.0	0.0	0.0	5.28774870926054E-4	0.0
15	0.0	0.0	0.0	7.402848192964756E-4	0.0
16	0.0	0.0	0.0	0.0014805696385929512	0.0
17	0.0	0.0	0.0	0.0024323644062598485	0.0
18	0.0	0.0	0.0	0.0030668942513711133	0.0
19	0.0	0.0	0.0	0.003912934044852799	0.0
20	0.0	0.0	0.0	0.0043359539415936425	0.0
21	0.0	0.0	0.0	0.005605013631816172	0.0
22	0.0	0.0	0.0	0.007825868089705599	0.0
23	0.0	0.0	0.0	0.010152477521780237	0.0
24	0.0	0.0	0.0	0.015122961308485144	0.0
25	0.0	0.0	0.0	0.018189855559856256	0.0
26	0.0	0.0	0.0	0.025486948778635804	0.0
27	0.0	0.0	0.0	0.06451053425297859	0.0
28	0.0	1.057549741852108E-4	0.0	0.19109923835267592	0.0
29	0.0	1.057549741852108E-4	0.0	0.36834457508708923	0.0
30	0.0	1.057549741852108E-4	0.0	0.6004767434236269	0.0
31	0.0	1.057549741852108E-4	0.0	1.1953484732154376	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGTGT	45	4.00727E-6	28.777777	14
GAATAGA	35	8.868225E-4	26.428572	1
GCACTAT	220	0.0	24.386366	13
GGCACTA	215	0.0	24.093023	12
ACTATTC	225	0.0	23.844444	15
CTATTCA	230	0.0	22.521738	16
CTTACAC	230	0.0	22.521738	3
ACTGATC	140	0.0	22.464287	8
CACTATT	240	0.0	21.583332	14
TTACACT	265	0.0	20.943396	4
TCTTACA	275	0.0	20.854544	2
GGTATCA	810	0.0	20.555555	1
CTCTTAC	290	0.0	20.413794	1
AACCGCT	65	6.900475E-5	19.923077	7
GTCTAGA	205	0.0	19.853657	1
ACATGTA	140	3.6379788E-12	19.82143	8
ACGGAGT	160	0.0	19.65625	26
TATTCAT	265	0.0	19.54717	17
CTTATAC	1155	0.0	19.380953	37
AACGGAG	165	0.0	19.060606	25
>>END_MODULE