FastQCFastQC Report
Fri 10 Feb 2017
ERR1631561.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631561.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109078
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35953.295806670456004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33353.057445131007169No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25212.311190157501971No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11151.0222042941748106No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA10510.9635306844643282No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA7120.6527439080291169No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4790.4391352976768918No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA4790.4391352976768918No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA4110.3767945873595042No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.35204165826289446No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC3690.3382900309870001No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.33645648068354755No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT3590.32912227946973727No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT3450.3162874273455692No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3450.3162874273455692No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA3390.31078677643521146No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT3330.30528612552485374No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA3110.28511707218687543No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC3100.28420029703514915No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA2860.26219769339371823No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT2780.25486349217990795No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG2520.23102733823502447No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2500.2291937879315719No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.21727571095913017No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2270.20810795944186727No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT2230.20444085883496213No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC2100.1925227818625204No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2000.18335503034525752No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT1880.17235372852454206No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.16868662791763692No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT1830.16776985276591064No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA1720.15768532609692149No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT1720.15768532609692149No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG1710.15676855094519518No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.1558517757934689No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT1700.1558517757934689No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC1680.15401822549001631No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.14301692366930088No Hit
ATCACCGACTGCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAA1550.14210014851757458ABI Solid3 Adapter B (95% over 23bp)
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC1530.14026659821412202No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA1370.12559819578650142ABI Solid3 Adapter B (100% over 21bp)
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT1360.12468142063477511No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1350.12376464548304882No Hit
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA1330.12193109517959624No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1310.12009754487614369No Hit
GCCTCTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTG1300.11918076972441739No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT1240.11368011881405966No Hit
CCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGC1220.11184656851060709ABI Solid3 Adapter B (100% over 23bp)
CTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATA1220.11184656851060709No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT1210.1109297933588808No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC1210.1109297933588808No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT1210.1109297933588808No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1200.1100130182071545No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT1190.10909624305542823No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC1170.10726269275197564No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1150.10542914244852308No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT1120.10267881699334422No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT1100.10084526668989165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCCC1000.033.30000337
TATTGAC658.54925E-1131.3076930
ATTGACT658.54925E-1131.3076931
GTGGACC250.005474823529.631
TGACTGT702.0190782E-1029.07142833
TTGACTG754.5110937E-1027.13333332
TATACAC400.001920060423.12537
ACCGACC400.001920060423.12532
GGGCAGT7300.021.794527
CACTGTC855.0731614E-821.76470636
TTATACA956.9667294E-921.42105337
GGCAGTC7350.021.3945588
CAGTCGG7300.021.2876710
AGTCGGT7300.021.2876711
GCAGTCG7450.021.1073829
TGGGCAG7550.021.0728456
GCACTGT450.003803975220.55555535
TCGGTGA7950.020.01257713
GTCGGTG7950.019.77987312
GGTGATT8150.019.52147515
Produced by FastQC (version 0.11.5)