##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631561.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	109078
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94479180036304	34.0	33.0	34.0	31.0	34.0
2	33.02932763710373	34.0	33.0	34.0	31.0	34.0
3	33.010808779038854	34.0	33.0	34.0	31.0	34.0
4	36.34013274904197	37.0	37.0	37.0	35.0	37.0
5	36.375245237353084	37.0	37.0	37.0	35.0	37.0
6	36.48951209226425	37.0	37.0	37.0	35.0	37.0
7	36.487385173912244	37.0	37.0	37.0	35.0	37.0
8	36.52105832523515	37.0	37.0	37.0	35.0	37.0
9	38.309237426428794	39.0	39.0	39.0	37.0	39.0
10	38.29349639707365	39.0	39.0	39.0	37.0	39.0
11	38.34456077302481	39.0	39.0	39.0	37.0	39.0
12	38.34608261977667	39.0	39.0	39.0	37.0	39.0
13	38.356029630172905	39.0	39.0	39.0	37.0	39.0
14	39.72967967876199	41.0	40.0	41.0	37.0	41.0
15	39.68620620106713	41.0	39.0	41.0	37.0	41.0
16	39.56554942334843	41.0	39.0	41.0	37.0	41.0
17	39.60036854361099	41.0	39.0	41.0	37.0	41.0
18	39.76004327178716	41.0	40.0	41.0	38.0	41.0
19	39.81850602321275	41.0	40.0	41.0	38.0	41.0
20	39.853517666257176	41.0	40.0	41.0	38.0	41.0
21	39.836117273877406	41.0	40.0	41.0	37.0	41.0
22	39.74840939511176	41.0	40.0	41.0	37.0	41.0
23	39.66892498945709	41.0	40.0	41.0	37.0	41.0
24	39.604897412860524	41.0	40.0	41.0	36.0	41.0
25	39.57710079026018	41.0	40.0	41.0	36.0	41.0
26	39.36326298612003	41.0	39.0	41.0	35.0	41.0
27	39.2045142008471	41.0	39.0	41.0	35.0	41.0
28	39.024661251581435	41.0	38.0	41.0	35.0	41.0
29	38.80522195126423	40.0	38.0	41.0	35.0	41.0
30	38.5200223693137	40.0	37.0	41.0	35.0	41.0
31	38.18352004987257	40.0	36.0	41.0	35.0	41.0
32	37.88600817763435	40.0	35.0	41.0	35.0	41.0
33	37.658886301545685	40.0	35.0	41.0	35.0	41.0
34	37.44603861456939	40.0	35.0	41.0	35.0	41.0
35	37.224894112469975	40.0	35.0	41.0	34.0	41.0
36	36.980225159977266	39.0	35.0	41.0	33.0	41.0
37	36.721373695887344	39.0	35.0	41.0	33.0	41.0
38	36.44988907020664	39.0	35.0	41.0	33.0	41.0
39	36.09934175544106	39.0	35.0	41.0	32.0	41.0
40	35.724518234657765	39.0	35.0	41.0	30.0	41.0
41	35.27889216890665	38.0	35.0	41.0	25.0	41.0
42	34.81207942939915	38.0	35.0	41.0	21.0	41.0
43	33.81725004125488	37.0	35.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	2.0
17	6.0
18	2.0
19	8.0
20	10.0
21	25.0
22	51.0
23	58.0
24	113.0
25	170.0
26	295.0
27	456.0
28	756.0
29	1077.0
30	1403.0
31	1588.0
32	1815.0
33	2298.0
34	3239.0
35	4442.0
36	6939.0
37	20051.0
38	20569.0
39	43704.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.24193696254057	14.810502576138177	16.644052879590753	23.303507581730504
2	18.857148095858008	21.452538550395133	34.705440143750344	24.984873209996515
3	20.288234107702745	24.153358147380775	30.03172042024973	25.526687324666753
4	12.995287775720127	19.935275674288125	31.072260217459064	35.99717633253268
5	12.760593336878198	41.200792093731096	29.160784026109752	16.877830543280954
6	26.392123067896367	30.444269238526562	22.7103540585636	20.45325363501348
7	30.217825776050166	33.307358037367756	17.5699957828343	18.904820403747777
8	36.525238819927026	28.170666862245362	16.59271347109408	18.71138084673353
9	32.638112176607564	12.575404756229489	18.082473092649295	36.70400997451365
10	22.017272043858522	28.339353490163006	22.072278552962104	27.571095913016375
11	31.049340838665906	28.242175324080016	15.148792607125177	25.5596912301289
12	25.016960340306937	32.060543831020006	24.353215130457105	18.569280698215955
13	35.00430884321311	19.095509635306847	26.1665963805717	19.733585140908342
14	23.716973175159062	22.77819541979134	27.44824804268505	26.056583362364545
15	32.782962650580316	24.449476521388362	22.20062707420378	20.566933753827538
16	22.078695979024186	26.449879902455127	28.948092190909257	22.523331927611434
17	17.39764205430976	32.3392434771448	28.616219585984343	21.646894882561103
18	20.880470855717924	22.63334494581859	32.71878838995948	23.76739580850401
19	19.94902730156402	30.69821595555474	34.21679898788023	15.135957755001009
20	22.663598525825556	23.01839050954363	36.321714736243784	17.996296228387028
21	26.12717504904747	21.403949467353637	33.95826839509342	18.510607088505473
22	24.414639065622765	22.402317607583562	30.119730834815456	23.063312491978216
23	20.283650231944115	25.73021140834999	30.85865160710684	23.127486752599058
24	19.35404022809366	25.74396303562588	35.364601477841546	19.537395258438917
25	18.404261170905222	25.594528685894495	32.08988063587525	23.911329507325032
26	23.340178587799556	24.057096756449514	31.52606391756358	21.076660738187353
27	20.547681475641284	24.429307468050386	30.481857019747338	24.541154036560993
28	18.3510882121051	25.843891527164047	33.79508241808614	22.009937842644714
29	17.907369038669575	26.64515300977282	35.73956251489759	19.707915436660002
30	17.077687526357284	27.706778635471863	38.01774876693742	17.19778507123343
31	17.914703239883387	29.53116118740718	32.74262454390436	19.811511028805075
32	16.682557435963254	29.311135150992868	36.91395148425897	17.092355928784905
33	18.796640935844074	26.28944425090302	36.411558701112966	18.502356112139935
34	19.568565613597606	28.309099910156032	30.60195456462348	21.520379911622875
35	18.156731879939127	30.22699352756743	31.215277141128368	20.40099745136508
36	15.17721263682869	29.863033792332093	33.73182493261703	21.227928638222192
37	16.875080217825776	29.56416509286932	33.2844386585746	20.276316030730303
38	17.280294834888796	25.681622325308496	35.524120354241916	21.513962485560793
39	18.95065916133409	25.76229853866041	35.09965345899265	20.187388841012854
40	17.252791580337007	25.159060488824508	32.43825519353123	25.14989273730725
41	13.84422156621867	26.848677093456057	35.87341168704963	23.43368965327564
42	14.721575386420726	25.85305927868131	34.351564935184	25.073800399713964
43	13.560938044335247	29.48348887951741	35.9210839949394	21.034489081207944
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	17.5
2	33.0
3	123.0
4	213.0
5	213.0
6	328.5
7	444.0
8	464.0
9	484.0
10	824.5
11	1165.0
12	1165.0
13	2195.5
14	3226.0
15	5078.5
16	6931.0
17	6066.0
18	5201.0
19	5201.0
20	5885.5
21	6570.0
22	4464.5
23	2359.0
24	1958.0
25	1557.0
26	1557.0
27	1495.5
28	1434.0
29	1479.0
30	1524.0
31	1554.0
32	1584.0
33	1584.0
34	1530.0
35	1476.0
36	1404.5
37	1333.0
38	1547.0
39	1761.0
40	1761.0
41	2067.0
42	2373.0
43	3122.0
44	3871.0
45	5078.0
46	6285.0
47	6285.0
48	9643.0
49	13001.0
50	12672.5
51	12344.0
52	9185.0
53	6026.0
54	6026.0
55	4828.0
56	3630.0
57	3548.0
58	3466.0
59	3436.0
60	3406.0
61	3406.0
62	3430.5
63	3455.0
64	3335.5
65	3216.0
66	3118.5
67	3021.0
68	3021.0
69	2756.5
70	2492.0
71	2219.5
72	1947.0
73	1690.5
74	1434.0
75	1434.0
76	1160.5
77	887.0
78	696.0
79	505.0
80	376.0
81	247.0
82	247.0
83	185.0
84	123.0
85	77.0
86	31.0
87	24.0
88	17.0
89	17.0
90	9.0
91	1.0
92	1.5
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	109078.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.077614184345144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.02169407212934	47.25059131997286
2	5.6256045322647505	5.971873338345037
3	1.720326101975957	2.7393241533581474
4	0.8359817603979549	1.7748766937420928
5	0.5492607434019622	1.4576724912447971
6	0.3644465938924969	1.16063734208548
7	0.24354014094237947	0.9048570747538459
8	0.23663120077380131	1.0047855662920113
9	0.14681497858228545	0.7013329910706101
>10	1.032886555202432	10.696932470342324
>50	0.11227027773939478	4.1291552833752
>100	0.10017963244438305	10.90504042978419
>500	0.001727235042144535	0.6527439080291169
>1k	0.008636175210722675	10.650176937604282
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3595	3.295806670456004	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3335	3.057445131007169	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2521	2.311190157501971	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1115	1.0222042941748106	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1051	0.9635306844643282	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	712	0.6527439080291169	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	479	0.4391352976768918	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	479	0.4391352976768918	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	411	0.3767945873595042	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	384	0.35204165826289446	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	369	0.3382900309870001	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	367	0.33645648068354755	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	359	0.32912227946973727	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	345	0.3162874273455692	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	345	0.3162874273455692	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	339	0.31078677643521146	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	333	0.30528612552485374	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	311	0.28511707218687543	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	310	0.28420029703514915	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	286	0.26219769339371823	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	278	0.25486349217990795	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	252	0.23102733823502447	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	250	0.2291937879315719	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	237	0.21727571095913017	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	227	0.20810795944186727	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	223	0.20444085883496213	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	210	0.1925227818625204	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	200	0.18335503034525752	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	188	0.17235372852454206	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	184	0.16868662791763692	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	183	0.16776985276591064	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	172	0.15768532609692149	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	172	0.15768532609692149	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	171	0.15676855094519518	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	170	0.1558517757934689	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	170	0.1558517757934689	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	168	0.15401822549001631	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	156	0.14301692366930088	No Hit
ATCACCGACTGCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAA	155	0.14210014851757458	ABI Solid3 Adapter B (95% over 23bp)
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	153	0.14026659821412202	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	137	0.12559819578650142	ABI Solid3 Adapter B (100% over 21bp)
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT	136	0.12468142063477511	No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	135	0.12376464548304882	No Hit
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA	133	0.12193109517959624	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	131	0.12009754487614369	No Hit
GCCTCTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTG	130	0.11918076972441739	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	124	0.11368011881405966	No Hit
CCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGC	122	0.11184656851060709	ABI Solid3 Adapter B (100% over 23bp)
CTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATA	122	0.11184656851060709	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	121	0.1109297933588808	No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC	121	0.1109297933588808	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	121	0.1109297933588808	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	120	0.1100130182071545	No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT	119	0.10909624305542823	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC	117	0.10726269275197564	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	115	0.10542914244852308	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT	112	0.10267881699334422	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	110	0.10084526668989165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.167751517262876E-4	0.0
10	0.0	0.0	0.0	9.167751517262876E-4	0.0
11	0.0	0.0	0.0	9.167751517262876E-4	0.0
12	0.0	0.0	0.0	0.0018335503034525751	0.0
13	0.0	0.0	0.0	0.0018335503034525751	0.0
14	0.0	0.0	0.0	0.002750325455178863	0.0
15	0.0	0.0	0.0	0.008250976365536589	0.0
16	0.0	0.0	0.0	0.013751627275894315	0.0
17	0.0	0.0	0.0	0.024752929096609765	0.0
18	0.0	0.0	0.0	0.025669704248336055	0.0
19	0.0	0.0	0.0	0.02750325455178863	0.0
20	0.0	0.0	0.0	0.029336804855241202	0.0
21	0.0	0.0	0.0	0.039421331524230364	0.0
22	0.0	0.0	0.0	0.05317295880012468	0.0
23	0.0	0.0	0.0	0.08526008911054475	0.0
24	0.0	0.0	0.0	0.13018207154513284	0.0
25	0.0	0.0	0.0	0.13843304791066943	0.0
26	0.0	0.0	0.0	0.1558517757934689	0.0
27	0.0	0.0	0.0	0.20352408368323585	0.0
28	0.0	0.0	0.0	0.3447074570490841	0.0
29	0.0	0.0	0.0	0.6197400025669704	0.0
30	0.0	0.0	0.0	1.0377894717541576	0.0
31	0.0	0.0	0.0	1.8473019307284695	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCCCC	100	0.0	33.300003	37
TATTGAC	65	8.54925E-11	31.30769	30
ATTGACT	65	8.54925E-11	31.30769	31
GTGGACC	25	0.0054748235	29.6	31
TGACTGT	70	2.0190782E-10	29.071428	33
TTGACTG	75	4.5110937E-10	27.133333	32
TATACAC	40	0.0019200604	23.125	37
ACCGACC	40	0.0019200604	23.125	32
GGGCAGT	730	0.0	21.79452	7
CACTGTC	85	5.0731614E-8	21.764706	36
TTATACA	95	6.9667294E-9	21.421053	37
GGCAGTC	735	0.0	21.394558	8
CAGTCGG	730	0.0	21.28767	10
AGTCGGT	730	0.0	21.28767	11
GCAGTCG	745	0.0	21.107382	9
TGGGCAG	755	0.0	21.072845	6
GCACTGT	45	0.0038039752	20.555555	35
TCGGTGA	795	0.0	20.012577	13
GTCGGTG	795	0.0	19.779873	12
GGTGATT	815	0.0	19.521475	15
>>END_MODULE