FastQCFastQC Report
Fri 10 Feb 2017
ERR1631560.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631560.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49677
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1150.23149546067596674No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1110.22344344465245486No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC910.18318336453489542No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC780.1570143124584818No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC770.15500130845260382No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC690.13889727640558006No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT550.11071522032328844No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC530.1066892123115325No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG510.10266320429977654No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC510.10266320429977654No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT500.1006502002938986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA556.061455E-726.909091
GGGCATC500.0069428118.532
GGCATCA500.0069428118.533
GTCTGTC500.0069428118.530
CTAAGGG701.18966185E-418.528
GCATCAC500.0069428118.534
TAAGGGC701.18966185E-418.529
GTATCAA852.639195E-517.4117642
CACACGC650.001551341817.0769238
GCACACG650.001551341817.0769237
CTTATAC1452.75395E-916.58620837
TCTAAGG904.3098E-516.44444527
CGCTGAG803.3017786E-416.187512
AGGCACA803.3017786E-416.18755
TCTTATA2400.016.187537
ACGCTGA700.002545959315.85714311
ATCTAAG956.844833E-515.57894826
CGGACAT956.844833E-515.57894821
GGACATC1001.0603E-414.822
ACATCTA1001.0603E-414.824