##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631560.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49677
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85458059061538	34.0	31.0	34.0	31.0	34.0
2	33.02560541095477	34.0	33.0	34.0	31.0	34.0
3	33.113573686011634	34.0	33.0	34.0	31.0	34.0
4	36.41457817501057	37.0	37.0	37.0	35.0	37.0
5	36.37113754856372	37.0	37.0	37.0	35.0	37.0
6	36.4083579926324	37.0	37.0	37.0	35.0	37.0
7	36.42631398836484	37.0	37.0	37.0	35.0	37.0
8	36.410250216397934	37.0	37.0	37.0	35.0	37.0
9	38.22881816534815	39.0	39.0	39.0	37.0	39.0
10	38.17237353302333	39.0	38.0	39.0	37.0	39.0
11	38.22912011594903	39.0	39.0	39.0	37.0	39.0
12	38.160537069468766	39.0	38.0	39.0	37.0	39.0
13	38.20804396400749	39.0	38.0	39.0	37.0	39.0
14	39.68516617348069	41.0	40.0	41.0	38.0	41.0
15	39.69627795559313	41.0	40.0	41.0	38.0	41.0
16	39.65666203675745	41.0	40.0	41.0	37.0	41.0
17	39.638665780944905	41.0	40.0	41.0	37.0	41.0
18	39.60853111097691	41.0	40.0	41.0	37.0	41.0
19	39.615053243955956	41.0	40.0	41.0	37.0	41.0
20	39.60027779455281	41.0	40.0	41.0	37.0	41.0
21	39.55258972965356	41.0	39.0	41.0	37.0	41.0
22	39.48551643617771	41.0	39.0	41.0	37.0	41.0
23	39.42603216780402	41.0	39.0	41.0	37.0	41.0
24	39.37639148901906	41.0	39.0	41.0	37.0	41.0
25	39.34444511544578	41.0	39.0	41.0	36.0	41.0
26	39.28298810314632	40.0	39.0	41.0	36.0	41.0
27	39.21231153249995	40.0	39.0	41.0	36.0	41.0
28	39.09998590897196	40.0	39.0	41.0	36.0	41.0
29	39.0356503009441	40.0	39.0	41.0	35.0	41.0
30	39.00148962296435	40.0	39.0	41.0	35.0	41.0
31	38.99325643658031	40.0	39.0	41.0	35.0	41.0
32	38.93351047768585	40.0	38.0	41.0	35.0	41.0
33	38.83167260502849	40.0	38.0	41.0	35.0	41.0
34	38.77903255027478	40.0	38.0	41.0	35.0	41.0
35	38.6802745737464	40.0	38.0	41.0	35.0	41.0
36	38.62127342633412	40.0	38.0	41.0	35.0	41.0
37	38.57652434728345	40.0	38.0	41.0	35.0	41.0
38	38.52048231575981	40.0	38.0	41.0	35.0	41.0
39	38.428689333091775	40.0	38.0	41.0	35.0	41.0
40	38.34114378887614	40.0	38.0	41.0	34.0	41.0
41	38.28999335708678	40.0	37.0	41.0	34.0	41.0
42	38.16007407854742	40.0	37.0	41.0	34.0	41.0
43	37.26984318698794	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	0.0
19	3.0
20	7.0
21	8.0
22	15.0
23	20.0
24	37.0
25	47.0
26	72.0
27	110.0
28	131.0
29	189.0
30	247.0
31	393.0
32	467.0
33	693.0
34	1068.0
35	1600.0
36	2626.0
37	5061.0
38	12963.0
39	23919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.50906858304648	17.46683575900316	12.076011031261952	26.948084626688406
2	22.434929645509992	18.17138716106045	29.991746683575897	29.401936509853655
3	22.447007669545265	18.82360045896491	27.314451355758194	31.414940515731626
4	16.554944944340438	14.187652233427944	31.237796163214366	38.01960665901725
5	18.576000966241924	32.79183525575216	31.429031543772773	17.203132234233145
6	39.3039032147674	31.19351007508505	13.555568975582261	15.947017734565291
7	32.42748153068825	26.891720514523826	18.525675866094975	22.15512208869296
8	30.434607564869054	28.64907301165529	18.076775972784187	22.839543450691465
9	27.386919499969803	13.676349215934941	17.55742093926767	41.37931034482759
10	20.907059605048612	22.863699498762003	28.765827243996217	27.463413652193168
11	40.64657688668801	19.530164865028084	17.60573303540874	22.217525212875174
12	25.812750367373233	22.692594158262374	23.813837389536406	27.68081808482799
13	33.60307587012098	16.26507236749401	22.01219880427562	28.119652958109388
14	25.732230207138112	18.33041447752481	20.528614851943555	35.40874046339352
15	29.577067858365037	24.3352054270588	18.445155705859857	27.642571008716306
16	29.38784548181251	22.336292449221972	20.152183102844376	28.12367896612114
17	28.33504438673833	22.551683877850916	20.589004972119895	28.524266763290857
18	28.073353865974195	20.442055679690803	22.344344465245484	29.140245989089518
19	28.781931276043238	22.088692956498985	21.51699981882964	27.612375948628138
20	29.798498299011616	20.001207802403524	20.80037039273708	29.399923505847774
21	30.768766229844797	21.39018056645933	20.61517402419631	27.225879179499568
22	31.61624091631942	20.54471888399058	20.69368118042555	27.145359019264447
23	29.202649113271733	20.607122008172794	20.844656480866398	29.345572397689075
24	28.1277049741329	21.965899712140427	21.66394911125873	28.242446202467942
25	28.588682891478957	21.176802141836262	21.170763129818628	29.063751836866153
26	28.439720595043983	22.47921573363931	21.545181874911933	27.535881796404777
27	29.50661271815931	20.397769591561488	21.929665640034624	28.16595205024458
28	28.10354892606236	20.97147573323671	21.917587615999356	29.007387724701573
29	27.175554079352622	21.48680475874147	23.14350705557904	28.194134106326874
30	27.199710127423156	22.318175413169072	22.312136401151438	28.169978058256333
31	27.93043058155686	22.205447188839905	21.311673410230085	28.55244881937315
32	27.038669806952914	20.907059605048612	22.19336916480464	29.86090142319383
33	26.12073998027256	20.77218833665479	23.4031845723373	29.70388711073535
34	28.093483906032972	19.88847957807436	23.612536988948605	28.405499526944062
35	26.563600861565718	19.379189564587236	24.9109245727399	29.146285001107152
36	24.943132636833948	21.17881514584214	24.914950580751654	28.963101636572258
37	26.698472129959537	18.356583529601224	26.15496104837249	28.78998329206675
38	25.28936932584496	18.475350765948022	26.869577470459166	29.365702437747853
39	25.267226281780303	17.945930712402117	28.465889647120395	28.32095335869718
40	23.354872476196228	18.831652474988424	29.16842804517181	28.645047003643537
41	22.285967349075026	18.942367695311713	30.541296777180587	28.230368178432673
42	22.59596996598023	19.075225959699658	30.843247378062284	27.485556696257824
43	21.160698109789237	18.76924935080621	30.176943052116673	29.893109487287877
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	3.5
7	6.0
8	4.5
9	3.0
10	4.0
11	5.0
12	5.0
13	13.5
14	22.0
15	34.0
16	46.0
17	43.0
18	40.0
19	40.0
20	43.5
21	47.0
22	43.5
23	40.0
24	52.5
25	65.0
26	65.0
27	93.0
28	121.0
29	179.0
30	237.0
31	269.0
32	301.0
33	301.0
34	446.0
35	591.0
36	730.0
37	869.0
38	1078.0
39	1287.0
40	1287.0
41	1494.5
42	1702.0
43	1947.5
44	2193.0
45	2442.0
46	2691.0
47	2691.0
48	3001.0
49	3311.0
50	3500.5
51	3690.0
52	4005.0
53	4320.0
54	4320.0
55	4274.5
56	4229.0
57	4308.5
58	4388.0
59	4435.0
60	4482.0
61	4482.0
62	4267.5
63	4053.0
64	3820.5
65	3588.0
66	3151.0
67	2714.0
68	2714.0
69	2331.0
70	1948.0
71	1579.0
72	1210.0
73	857.5
74	505.0
75	505.0
76	398.0
77	291.0
78	263.5
79	236.0
80	209.0
81	182.0
82	182.0
83	141.5
84	101.0
85	86.0
86	71.0
87	58.5
88	46.0
89	46.0
90	38.5
91	31.0
92	18.0
93	5.0
94	6.0
95	7.0
96	7.0
97	3.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	49677.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.74408680073273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.30746705710104	60.70616180526199
2	5.376281112737921	7.391750709583913
3	2.0175695461200585	4.160879280149767
4	1.0746705710102489	2.9550898806288624
5	0.7115666178623719	2.445799867141736
6	0.5387994143484627	2.222356422489281
7	0.2840409956076135	1.3668297199911428
8	0.2430453879941435	1.3366346599029733
9	0.2459736456808199	1.5218310284437466
>10	1.171303074670571	14.380900617992229
>50	0.02342606149341142	1.0568271030859353
>100	0.005856515373352855	0.4549389053284216
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	115	0.23149546067596674	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	111	0.22344344465245486	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	91	0.18318336453489542	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	78	0.1570143124584818	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	77	0.15500130845260382	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	69	0.13889727640558006	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	55	0.11071522032328844	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	53	0.1066892123115325	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	51	0.10266320429977654	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	51	0.10266320429977654	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	50	0.1006502002938986	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.002013004005877972	0.0
15	0.0	0.0	0.0	0.002013004005877972	0.0
16	0.0	0.0	0.0	0.002013004005877972	0.0
17	0.0	0.0	0.0	0.004026008011755944	0.0
18	0.0	0.0	0.0	0.004026008011755944	0.0
19	0.0	0.0	0.0	0.004026008011755944	0.0
20	0.0	0.0	0.0	0.006039012017633915	0.0
21	0.0	0.0	0.0	0.006039012017633915	0.0
22	0.0	0.0	0.0	0.006039012017633915	0.0
23	0.0	0.0	0.0	0.010065020029389858	0.0
24	0.0	0.0	0.0	0.016104032047023775	0.0
25	0.0	0.0	0.0	0.02415604807053566	0.0
26	0.0	0.0	0.0	0.03623407210580349	0.0
27	0.0	0.0	0.0	0.08857217625863076	0.0
28	0.0	0.002013004005877972	0.0	0.37240574108742475	0.0
29	0.0	0.002013004005877972	0.0	0.8977997866215753	0.0
30	0.0	0.002013004005877972	0.0	1.4735189323026754	0.0
31	0.0	0.002013004005877972	0.0	2.8222316162409165	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	55	6.061455E-7	26.90909	1
GGGCATC	50	0.00694281	18.5	32
GGCATCA	50	0.00694281	18.5	33
GTCTGTC	50	0.00694281	18.5	30
CTAAGGG	70	1.18966185E-4	18.5	28
GCATCAC	50	0.00694281	18.5	34
TAAGGGC	70	1.18966185E-4	18.5	29
GTATCAA	85	2.639195E-5	17.411764	2
CACACGC	65	0.0015513418	17.076923	8
GCACACG	65	0.0015513418	17.076923	7
CTTATAC	145	2.75395E-9	16.586208	37
TCTAAGG	90	4.3098E-5	16.444445	27
CGCTGAG	80	3.3017786E-4	16.1875	12
AGGCACA	80	3.3017786E-4	16.1875	5
TCTTATA	240	0.0	16.1875	37
ACGCTGA	70	0.0025459593	15.857143	11
ATCTAAG	95	6.844833E-5	15.578948	26
CGGACAT	95	6.844833E-5	15.578948	21
GGACATC	100	1.0603E-4	14.8	22
ACATCTA	100	1.0603E-4	14.8	24
>>END_MODULE