FastQCFastQC Report
Fri 10 Feb 2017
ERR1631559.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631559.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302060
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT71462.3657551479838443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52691.74435542607429No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49231.6298086472886182No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18910.6260345626696683No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA14430.4777196583460239No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA6730.2228034165397603No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA6710.2221412964311726No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6620.21916175594252799No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6010.19896709263060322No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA5280.17479970866715222No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5050.1671853274183937No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4910.1625504866582798No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC4820.15957094616963519No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC4610.15261868502946435No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA4610.15261868502946435No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA4580.1516255048665828No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4490.14864596437793817No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT4460.14765278421505662No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC4440.1469906641064689No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT4340.1436800635635304No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4320.14301794345494273No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT4000.13242402171753956No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT3940.13043766139177645No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT3920.12977554128318877No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.12514070052307488No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA3730.12348540025160565No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA3630.12017479970866715No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA3080.10196649672250546No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3060.10130437661391777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGT308.291698E-637.01
TCGAAAC200.001839788937.026
CTCGAAT401.5949026E-632.37533
TCGATAA250.00549000929.624
ATACTGG652.9722287E-928.4615386
GATATAT405.9258622E-527.751
CCAAGCA405.9258622E-527.7536
TGAGGAT405.9258622E-527.7532
ATGGAGG809.713403E-1025.43756
TGTTTAC451.3200594E-424.66666614
ATATATG451.3200594E-424.6666662
ATTATTG451.3200594E-424.66666619
GCCTTAT6850.023.76642226
TGCCTGG551.8966452E-523.54545412
GCTCGAA551.8966452E-523.54545432
GGCTCGA551.8966452E-523.54545431
TGACTGT952.7830538E-1023.36842233
GGATACC400.001927910623.12525
GAGCCGC400.001927910623.12516
ATACACT400.001927910623.1254
Produced by FastQC (version 0.11.5)