##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631559.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	302060
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90929616632457	34.0	33.0	34.0	31.0	34.0
2	33.00853803880024	34.0	33.0	34.0	31.0	34.0
3	33.05688604912931	34.0	33.0	34.0	31.0	34.0
4	36.36271932728597	37.0	37.0	37.0	35.0	37.0
5	36.367986492749786	37.0	37.0	37.0	35.0	37.0
6	36.46132556445739	37.0	37.0	37.0	35.0	37.0
7	36.47759716612593	37.0	37.0	37.0	35.0	37.0
8	36.50015559822552	37.0	37.0	37.0	35.0	37.0
9	38.30055287029067	39.0	39.0	39.0	37.0	39.0
10	38.26070979275641	39.0	39.0	39.0	37.0	39.0
11	38.322508773091435	39.0	39.0	39.0	37.0	39.0
12	38.289396146460966	39.0	39.0	39.0	37.0	39.0
13	38.32918956498709	39.0	39.0	39.0	37.0	39.0
14	39.73459246507316	41.0	40.0	41.0	37.0	41.0
15	39.683920413162944	41.0	40.0	41.0	37.0	41.0
16	39.62167450175462	41.0	39.0	41.0	37.0	41.0
17	39.664079984109115	41.0	39.0	41.0	37.0	41.0
18	39.749973515195656	41.0	40.0	41.0	38.0	41.0
19	39.79122359796067	41.0	40.0	41.0	38.0	41.0
20	39.77561080580017	41.0	40.0	41.0	38.0	41.0
21	39.7507250215189	41.0	40.0	41.0	37.0	41.0
22	39.66079917897107	41.0	40.0	41.0	37.0	41.0
23	39.592829239223995	41.0	40.0	41.0	37.0	41.0
24	39.564361385155266	41.0	40.0	41.0	37.0	41.0
25	39.55185393630405	41.0	40.0	41.0	36.0	41.0
26	39.38712176388797	41.0	39.0	41.0	36.0	41.0
27	39.296414619611994	41.0	39.0	41.0	35.0	41.0
28	39.180702509435214	41.0	39.0	41.0	35.0	41.0
29	39.04190889227306	41.0	39.0	41.0	35.0	41.0
30	38.837303846917834	40.0	38.0	41.0	35.0	41.0
31	38.621601668542674	40.0	38.0	41.0	35.0	41.0
32	38.440571409653714	40.0	37.0	41.0	35.0	41.0
33	38.25768390386016	40.0	36.0	41.0	35.0	41.0
34	38.153654902999406	40.0	36.0	41.0	35.0	41.0
35	37.96199761636761	40.0	35.0	41.0	35.0	41.0
36	37.804548765145995	40.0	35.0	41.0	35.0	41.0
37	37.63054691120969	40.0	35.0	41.0	34.0	41.0
38	37.449549758326164	40.0	35.0	41.0	34.0	41.0
39	37.23114612990796	40.0	35.0	41.0	33.0	41.0
40	36.99185923326491	40.0	35.0	41.0	33.0	41.0
41	36.7249652386943	40.0	35.0	41.0	33.0	41.0
42	36.414616301397075	40.0	35.0	41.0	31.0	41.0
43	35.56866847646163	39.0	35.0	41.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	4.0
13	2.0
14	1.0
15	7.0
16	8.0
17	13.0
18	16.0
19	20.0
20	21.0
21	58.0
22	102.0
23	148.0
24	263.0
25	421.0
26	641.0
27	995.0
28	1618.0
29	2258.0
30	2960.0
31	3561.0
32	4224.0
33	5248.0
34	7622.0
35	10485.0
36	15900.0
37	42355.0
38	60298.0
39	142806.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.30027146924452	17.040323114612992	14.289876183539693	23.369529232602794
2	18.374826193471495	21.695689598093093	34.75302919949679	25.17645500893862
3	18.74793087466066	26.005760444944713	30.010262861683106	25.236045818711517
4	11.821492418724757	17.81963848242071	35.019863603257626	35.3390054955969
5	11.889359729854995	41.72382970270807	31.965172482288285	14.421638085148647
6	27.808382440574718	35.850824339535194	19.89604714295173	16.444746076938358
7	28.787327021121634	32.71204396477521	20.579024034959943	17.921604979143215
8	32.894789114745414	29.76693372177713	18.122889492153877	19.215387671323576
9	30.043037807058198	13.019267695159902	19.314043567503145	37.62365093027875
10	19.835132092961665	28.039462358471827	27.378004369992716	24.747401178573796
11	33.402966298086476	26.158710190028472	18.512547176057737	21.92577633582732
12	23.40793219890088	30.751506323247035	26.10242998079852	19.738131497053566
13	31.90194001191816	19.72058531417599	26.137522346553666	22.23995232735218
14	22.931867840826325	21.666225253260944	27.402171753956168	27.999735151956568
15	27.881215652519366	27.37734224988413	23.150698536714557	21.590743560881943
16	22.857048268555914	26.35800834271337	27.093954843408596	23.69098854532212
17	19.773554922863006	29.935112229358406	27.49255114877839	22.798781699000198
18	21.47685890220486	24.55439316692048	30.72435939879494	23.244388532079718
19	21.38647950738264	28.01297755412832	31.924452095610146	18.6760908428789
20	22.826921803615175	24.3650268158644	32.64450771369927	20.163543666821163
21	24.590147652784214	24.547440905780306	31.079586837052243	19.782824604383233
22	24.135602198238757	24.394160100642257	28.96080248957161	22.509435211547373
23	21.278222869628554	25.985896841687083	30.112229358405614	22.623650930278753
24	19.869893398662516	26.81288485731312	31.300072833211946	22.01714891081242
25	20.384029662980865	25.855459180295306	30.86439780176124	22.89611335496259
26	21.602992782890816	26.50797854730848	30.448255313513872	21.44077335628683
27	20.82069787459445	26.305038734026354	29.496126597364764	23.378136794014434
28	19.507382639210753	26.594716281533472	32.125736608620805	21.772164470634973
29	20.020856783420513	26.995961067337614	32.58160630338343	20.40157584585844
30	19.022710719724557	28.09574256770178	33.3533072899424	19.528239422631266
31	20.261868502946434	28.669800701847315	30.7051579156459	20.363172879560352
32	19.099847712375027	27.946103423160963	32.550155598225516	20.403893266238494
33	19.457061510958088	26.805270476064358	32.85208236774151	20.885585645236045
34	21.232867642190293	27.085347281996953	29.908296364960602	21.77348871085215
35	20.058928689664306	28.55558498311594	30.50652188306959	20.87896444415017
36	18.77011189829835	28.5681652651791	30.63033834337549	22.03138449314706
37	19.444481228894922	27.800436999271668	31.157054889757003	21.59802688207641
38	19.315036747666024	25.967688538700923	32.64351453353638	22.07376018009667
39	19.81924121035556	26.410977951400383	32.19559028007681	21.574190558167253
40	19.00648877706416	25.892206846321926	31.841687082036678	23.259617294577236
41	17.11150102628617	26.604648083162285	33.19439846388135	23.0894524266702
42	17.419055816725155	26.272594848705555	32.639210752830564	23.66913858173873
43	16.58014963914454	26.87578626762895	33.57478646626498	22.96927762696153
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19.0
1	42.0
2	65.0
3	289.0
4	513.0
5	513.0
6	743.0
7	973.0
8	960.0
9	947.0
10	1569.5
11	2192.0
12	2192.0
13	3838.5
14	5485.0
15	9080.0
16	12675.0
17	10984.5
18	9294.0
19	9294.0
20	10104.5
21	10915.0
22	7477.0
23	4039.0
24	3525.0
25	3011.0
26	3011.0
27	2988.0
28	2965.0
29	3749.0
30	4533.0
31	5345.5
32	6158.0
33	6158.0
34	7257.0
35	8356.0
36	9691.0
37	11026.0
38	12036.5
39	13047.0
40	13047.0
41	14832.5
42	16618.0
43	18244.5
44	19871.0
45	21829.0
46	23787.0
47	23787.0
48	27368.5
49	30950.0
50	29225.0
51	27500.0
52	23700.5
53	19901.0
54	19901.0
55	17033.5
56	14166.0
57	11939.5
58	9713.0
59	9451.0
60	9189.0
61	9189.0
62	8803.0
63	8417.0
64	7632.5
65	6848.0
66	6348.5
67	5849.0
68	5849.0
69	5002.0
70	4155.0
71	3675.0
72	3195.0
73	2498.0
74	1801.0
75	1801.0
76	1473.5
77	1146.0
78	965.0
79	784.0
80	713.5
81	643.0
82	643.0
83	576.5
84	510.0
85	421.5
86	333.0
87	302.0
88	271.0
89	271.0
90	204.5
91	138.0
92	92.0
93	46.0
94	29.0
95	12.0
96	12.0
97	7.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	302060.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.19144848302702
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.2226667906124	25.71971936327131
2	9.586247361632173	6.5553536562160035
3	3.9832073356961173	4.085748852470074
4	2.246784654898675	3.0728328712169475
5	1.529325346588841	2.61449244008399
6	1.0808041151207701	2.217255493343725
7	0.8011126240679474	1.917384071044527
8	0.6622663376124531	1.8115076291615337
9	0.5394024397797723	1.6598655658214254
>10	3.8477373978617684	25.25727945148005
>50	0.3526093721228876	8.228288302392006
>100	0.13684839654627648	8.556846053799157
>500	0.005993360432683642	1.2431914918108462
>1k	0.002996680216341821	2.820063776890702
>5k	0.0019977868108945473	4.240170980997707
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7146	2.3657551479838443	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5269	1.74435542607429	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4923	1.6298086472886182	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1891	0.6260345626696683	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1443	0.4777196583460239	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	673	0.2228034165397603	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	671	0.2221412964311726	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	662	0.21916175594252799	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	601	0.19896709263060322	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	528	0.17479970866715222	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	505	0.1671853274183937	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	491	0.1625504866582798	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	482	0.15957094616963519	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	461	0.15261868502946435	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	461	0.15261868502946435	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	458	0.1516255048665828	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	449	0.14864596437793817	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	446	0.14765278421505662	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	444	0.1469906641064689	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	434	0.1436800635635304	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	432	0.14301794345494273	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	400	0.13242402171753956	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	394	0.13043766139177645	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	392	0.12977554128318877	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	378	0.12514070052307488	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	373	0.12348540025160565	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	363	0.12017479970866715	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	308	0.10196649672250546	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	306	0.10130437661391777	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.310600542938489E-4	0.0
11	0.0	0.0	0.0	0.0023174203800569422	0.0
12	0.0	0.0	0.0	0.005628020922995431	0.0
13	0.0	0.0	0.0	0.006290141031583129	0.0
14	0.0	0.0	0.0	0.007945441303052374	0.0
15	0.0	0.0	0.0	0.011587101900284712	0.0
16	0.0	0.0	0.0	0.015559822551810898	0.0
17	0.0	0.0	0.0	0.021849963583394028	0.0
18	0.0	0.0	0.0	0.026153744289214062	0.0
19	0.0	0.0	0.0	0.028802224723564855	0.0
20	0.0	0.0	0.0	0.035092365755147986	0.0
21	0.0	0.0	0.0	0.04105144673243726	0.0
22	0.0	0.0	0.0	0.05760444944712971	0.0
23	0.0	0.0	0.0	0.08442031384493147	0.0
24	0.0	0.0	0.0	0.11553995894855326	0.0
25	0.0	0.0	0.0	0.12381646030589949	0.0
26	0.0	0.0	0.0	0.138052042640535	0.0
27	0.0	0.0	0.0	0.17479970866715222	0.0
28	0.0	0.0	0.0	0.297954048864464	0.0
29	0.0	0.0	0.0	0.512812024101172	0.0
30	0.0	0.0	0.0	0.8203668145401576	0.0
31	0.0	0.0	0.0	1.4318347348208964	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAGCGT	30	8.291698E-6	37.0	1
TCGAAAC	20	0.0018397889	37.0	26
CTCGAAT	40	1.5949026E-6	32.375	33
TCGATAA	25	0.005490009	29.6	24
ATACTGG	65	2.9722287E-9	28.461538	6
GATATAT	40	5.9258622E-5	27.75	1
CCAAGCA	40	5.9258622E-5	27.75	36
TGAGGAT	40	5.9258622E-5	27.75	32
ATGGAGG	80	9.713403E-10	25.4375	6
TGTTTAC	45	1.3200594E-4	24.666666	14
ATATATG	45	1.3200594E-4	24.666666	2
ATTATTG	45	1.3200594E-4	24.666666	19
GCCTTAT	685	0.0	23.766422	26
TGCCTGG	55	1.8966452E-5	23.545454	12
GCTCGAA	55	1.8966452E-5	23.545454	32
GGCTCGA	55	1.8966452E-5	23.545454	31
TGACTGT	95	2.7830538E-10	23.368422	33
GGATACC	40	0.0019279106	23.125	25
GAGCCGC	40	0.0019279106	23.125	16
ATACACT	40	0.0019279106	23.125	4
>>END_MODULE