FastQCFastQC Report
Fri 10 Feb 2017
ERR1631558.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631558.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053658
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAGAGCAATTGCTGCCAGCATTCAAGTGCGCTGGGCCTG17900.16988434577443534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15220.14444914763614No Hit
CTCTGTGGCCTATGCAGCTCCTGGCCCCCGGGGGATCATTATC15060.14293062834430148No Hit
CTCCCACACCTAGCCCAGAATGCTGTAGGCCACTAGGCGCAGG14990.14226627615412213No Hit
GTGTGGGAGTGGGTGGGCAGTCTCACTGCTTGGAGCGTCCAGC14970.1420764612426423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14630.13884960774748545No Hit
CTCTTACACTGGGCACTATTCATGCAGAGCTCACCGTTCTCCA14460.13723618099990698No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA14320.13590747661954827No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12590.1194884867765442No Hit
GGTCAAGGAGGTGGCCAGCCCGGGAGATGCGCTGGAGCAGGGG12100.1148380214452887No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12100.1148380214452887No Hit
ATTGTGAAGAGCGCAATCAGAAAACAGATATGCTGGTCAAGGA11140.10572690569425752No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT10890.10335421930075983No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC10860.1030694969335401No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA10790.10240514474336075No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC10660.10117134781874193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTGC303.5992765E-430.83333424
GACGTGT250.00549613929.5999986
GGTATCA8600.024.738371
ATACACA2750.022.87272637
GTCTAGA3500.022.7285731
AGTGCGC4750.021.03157829
GTGCGCT4600.020.91304430
TACCGTC1800.020.5555557
ATACCGT1950.019.9230776
AAGTGCG5050.019.78217728
TAGAACT3950.019.6708854
ACTGATC2650.019.547178
CTAGACA3600.019.5277774
GTTCTAG3700.019.5000021
AAGACGG2100.019.380955
TCTAGAC3450.019.3043463
ACCGTCG1950.018.974368
ACATGTA2450.018.877558
TTCAAGT5600.018.525
ACGGACC2200.018.58
Produced by FastQC (version 0.11.5)