##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631558.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1053658
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.01116965846603	34.0	33.0	34.0	31.0	34.0
2	33.151475146584566	34.0	33.0	34.0	31.0	34.0
3	33.23979033044878	34.0	33.0	34.0	31.0	34.0
4	36.46854577101868	37.0	37.0	37.0	35.0	37.0
5	36.454561157415405	37.0	37.0	37.0	35.0	37.0
6	36.51254581657426	37.0	37.0	37.0	35.0	37.0
7	36.53308473907093	37.0	37.0	37.0	35.0	37.0
8	36.52519603134983	37.0	37.0	37.0	35.0	37.0
9	38.36687710813186	39.0	39.0	39.0	37.0	39.0
10	38.329418084425875	39.0	39.0	39.0	37.0	39.0
11	38.3830056811603	39.0	39.0	39.0	37.0	39.0
12	38.344467559682556	39.0	39.0	39.0	37.0	39.0
13	38.37538176524071	39.0	39.0	39.0	37.0	39.0
14	39.90335194152182	41.0	40.0	41.0	38.0	41.0
15	39.89971318966875	41.0	40.0	41.0	38.0	41.0
16	39.87520713552215	41.0	40.0	41.0	38.0	41.0
17	39.87563896444577	41.0	40.0	41.0	38.0	41.0
18	39.843059133039375	41.0	40.0	41.0	38.0	41.0
19	39.8510028870848	41.0	40.0	41.0	38.0	41.0
20	39.82118676078955	41.0	40.0	41.0	38.0	41.0
21	39.79038264788005	41.0	40.0	41.0	38.0	41.0
22	39.753247258598144	41.0	40.0	41.0	38.0	41.0
23	39.707163045314516	41.0	40.0	41.0	38.0	41.0
24	39.67817641018243	41.0	40.0	41.0	37.0	41.0
25	39.679050507849794	41.0	40.0	41.0	37.0	41.0
26	39.59525386795336	41.0	40.0	41.0	37.0	41.0
27	39.544822893196844	41.0	40.0	41.0	37.0	41.0
28	39.49543400230435	41.0	40.0	41.0	37.0	41.0
29	39.464236972528084	41.0	39.0	41.0	37.0	41.0
30	39.41423687762063	41.0	39.0	41.0	36.0	41.0
31	39.36467430608414	41.0	39.0	41.0	36.0	41.0
32	39.288053618916194	41.0	39.0	41.0	36.0	41.0
33	39.22712303233117	41.0	39.0	41.0	35.0	41.0
34	39.18081009207921	41.0	39.0	41.0	35.0	41.0
35	39.09768919326765	41.0	39.0	41.0	35.0	41.0
36	39.03536251800869	40.0	39.0	41.0	35.0	41.0
37	38.977491747796726	40.0	39.0	41.0	35.0	41.0
38	38.90092136158032	40.0	39.0	41.0	35.0	41.0
39	38.83553012457553	40.0	38.0	41.0	35.0	41.0
40	38.75324061507624	40.0	38.0	41.0	35.0	41.0
41	38.68946280481902	40.0	38.0	41.0	35.0	41.0
42	38.56797651609915	40.0	38.0	41.0	35.0	41.0
43	37.77503041783957	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	0.0
13	0.0
14	2.0
15	0.0
16	3.0
17	5.0
18	8.0
19	29.0
20	59.0
21	88.0
22	171.0
23	329.0
24	501.0
25	847.0
26	1233.0
27	1789.0
28	2508.0
29	3573.0
30	4928.0
31	6567.0
32	8437.0
33	11748.0
34	17292.0
35	25872.0
36	43132.0
37	84313.0
38	217649.0
39	622573.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.39201334778458	16.659770058216232	11.306325202295241	27.64189139170395
2	20.106808850689692	18.874530445362726	32.938012144358034	28.080648559589545
3	21.44974934940939	19.554162735916208	27.76327802759529	31.23280988707911
4	15.628126014323431	14.08312754233347	31.577039229047756	38.711707214295345
5	17.204538854163307	33.767028770246135	31.50737715653466	17.5210552190559
6	37.41204451539304	31.824557873617437	14.637861621133233	16.125535989856292
7	32.297956262848096	28.066602256140037	18.75836371953708	20.877077761474787
8	29.33399642009077	30.576335015726166	18.802400779000397	21.287267785182667
9	28.23904910321945	13.542155044615994	18.145166648001535	40.07362920416302
10	18.732264169208605	24.644524124526175	30.273675139371598	26.349536566893622
11	39.06134628124116	19.804528604158087	19.561090980185224	21.57303413441553
12	24.785746418667156	23.65891019666723	25.92985579761175	25.625487587053865
13	32.38650491905343	17.113902233931693	23.36972717902773	27.129865667987147
14	24.68030423534012	18.52204415474471	22.27449513978919	34.52315647012598
15	27.109745287370284	25.394482839782928	19.569253021378856	27.926518851467936
16	27.236636555694542	23.93736867180812	21.67657816862777	27.149416603869568
17	27.182729120834274	23.893141797433323	22.434129480343717	26.489999601388686
18	27.44505332849938	22.06940012793525	23.56295875891418	26.922587784651185
19	27.031731358752083	23.05197701721052	23.485704089941898	26.4305875340955
20	27.044448957821228	22.096258937909642	23.180576619738094	27.678715484531036
21	27.68251178276063	22.511099426948782	23.608893967492296	26.19749482279829
22	28.80763967055724	22.293381723481435	22.393698904198516	26.505279701762813
23	27.179692082250597	22.250578460942734	23.088041850391683	27.481687606414983
24	26.99452763610204	22.88294683853774	22.848020894825456	27.274504630534768
25	27.08592351597957	22.40262020503807	22.835777833035007	27.675678445947355
26	26.879784522112487	23.063176096987828	22.82353477124456	27.233504609655125
27	27.68089835601305	22.31426136374421	23.190257180223565	26.814583100019174
28	26.687217294416214	22.553143429841562	23.633190276161713	27.12644899958051
29	26.40467779867851	22.734226855393306	23.551949494048355	27.309145851879833
30	25.757883487811036	22.891868139377294	24.374037875667437	26.976210497144237
31	26.995856340482394	22.52011563524407	22.960960767155946	27.523067257117585
32	26.392150014520844	22.391231310349276	23.565046722940462	27.651571952189418
33	26.349062029614924	22.538717496569095	23.36004661854226	27.75217385527372
34	27.14438650871535	22.195152506790627	23.82347972492023	26.83698125957379
35	26.265543468563802	22.188983522167536	24.517632856201917	27.027840153066744
36	25.577654229360952	22.005717225133772	25.07037387843114	27.346254667074138
37	26.438654667833394	21.16170522123877	25.3825245003597	27.017115610568137
38	25.346459667178532	20.361730276807087	26.490379231211648	27.801430824802736
39	26.138367477872325	20.686218867981832	26.182024907512684	26.99338874663316
40	24.74465149033178	21.259459900650874	26.32305738674219	27.672831222275157
41	24.548667594228867	21.49055955537755	27.07956471644499	26.881208133948586
42	24.845727930694782	21.127633444628145	27.247361098193153	26.779277526483924
43	23.50468558108988	20.762144832573757	27.341794016654358	28.391375569682005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	211.0
1	173.5
2	136.0
3	284.0
4	432.0
5	432.0
6	462.5
7	493.0
8	463.0
9	433.0
10	640.5
11	848.0
12	848.0
13	1324.0
14	1800.0
15	2457.5
16	3115.0
17	2916.0
18	2717.0
19	2717.0
20	3352.5
21	3988.0
22	3554.5
23	3121.0
24	3518.0
25	3915.0
26	3915.0
27	4601.0
28	5287.0
29	6758.5
30	8230.0
31	9958.0
32	11686.0
33	11686.0
34	14997.0
35	18308.0
36	22249.0
37	26190.0
38	30896.5
39	35603.0
40	35603.0
41	39653.5
42	43704.0
43	46214.0
44	48724.0
45	55031.5
46	61339.0
47	61339.0
48	66710.5
49	72082.0
50	74609.5
51	77137.0
52	81497.0
53	85857.0
54	85857.0
55	87987.5
56	90118.0
57	90654.5
58	91191.0
59	87435.0
60	83679.0
61	83679.0
62	81882.0
63	80085.0
64	73169.0
65	66253.0
66	57912.0
67	49571.0
68	49571.0
69	40454.0
70	31337.0
71	26248.5
72	21160.0
73	15212.0
74	9264.0
75	9264.0
76	6925.0
77	4586.0
78	3858.5
79	3131.0
80	2877.5
81	2624.0
82	2624.0
83	2229.0
84	1834.0
85	1708.0
86	1582.0
87	1392.0
88	1202.0
89	1202.0
90	859.5
91	517.0
92	312.5
93	108.0
94	76.5
95	45.0
96	45.0
97	29.0
98	13.0
99	7.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1053658.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.792370238655735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.5660743136167	32.02933723926182
2	7.623279179866009	5.914501367559962
3	2.699305178808974	3.1413733765043563
4	1.5197772075255407	2.3582304045840443
5	0.9635794880166004	1.868976612675715
6	0.6582453761780038	1.5320939004348202
7	0.4903306910678358	1.3314762795095587
8	0.3848419173878517	1.1943144114130964
9	0.2841533135002923	0.9920682487659735
>10	2.227860949940978	17.548740048263443
>50	0.31873738076235675	8.634174753605683
>100	0.2454264110908677	17.745258494597998
>500	0.014465692560579368	3.686621132084586
>1k	0.003922899677445252	2.0228337307390154
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAAGAGCAATTGCTGCCAGCATTCAAGTGCGCTGGGCCTG	1790	0.16988434577443534	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1522	0.14444914763614	No Hit
CTCTGTGGCCTATGCAGCTCCTGGCCCCCGGGGGATCATTATC	1506	0.14293062834430148	No Hit
CTCCCACACCTAGCCCAGAATGCTGTAGGCCACTAGGCGCAGG	1499	0.14226627615412213	No Hit
GTGTGGGAGTGGGTGGGCAGTCTCACTGCTTGGAGCGTCCAGC	1497	0.1420764612426423	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1463	0.13884960774748545	No Hit
CTCTTACACTGGGCACTATTCATGCAGAGCTCACCGTTCTCCA	1446	0.13723618099990698	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1432	0.13590747661954827	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1259	0.1194884867765442	No Hit
GGTCAAGGAGGTGGCCAGCCCGGGAGATGCGCTGGAGCAGGGG	1210	0.1148380214452887	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1210	0.1148380214452887	No Hit
ATTGTGAAGAGCGCAATCAGAAAACAGATATGCTGGTCAAGGA	1114	0.10572690569425752	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	1089	0.10335421930075983	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1086	0.1030694969335401	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	1079	0.10240514474336075	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1066	0.10117134781874193	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.490745573990802E-5	0.0
5	0.0	0.0	0.0	9.490745573990802E-5	0.0
6	0.0	0.0	0.0	9.490745573990802E-5	0.0
7	0.0	0.0	0.0	9.490745573990802E-5	0.0
8	0.0	0.0	0.0	9.490745573990802E-5	0.0
9	0.0	0.0	0.0	1.8981491147981603E-4	0.0
10	0.0	0.0	0.0	1.8981491147981603E-4	0.0
11	0.0	0.0	0.0	4.745372786995401E-4	0.0
12	0.0	0.0	0.0	4.745372786995401E-4	0.0
13	0.0	0.0	0.0	4.745372786995401E-4	0.0
14	0.0	0.0	0.0	5.694447344394481E-4	0.0
15	0.0	0.0	0.0	8.541671016591722E-4	0.0
16	0.0	0.0	0.0	0.0014236118360986202	0.0
17	0.0	0.0	0.0	0.0018032416590582523	0.0
18	0.0	0.0	0.0	0.002087964026277976	0.0
19	0.0	0.0	0.0	0.0027523162164573324	0.0
20	0.0	0.0	0.0	0.0031319460394169645	0.0
21	0.0	0.0	0.0	0.0037013907738564127	0.0
22	0.0	0.0	0.0	0.004745372786995401	0.0
23	0.0	0.0	0.0	0.006548614446053653	0.0
24	0.0	9.490745573990802E-5	0.0	0.009395838118250894	0.0
25	0.0	9.490745573990802E-5	0.0	0.010914357410089422	0.0
26	0.0	9.490745573990802E-5	0.0	0.018032416590582523	0.0
27	0.0	9.490745573990802E-5	0.0	0.059981512027621864	0.0
28	0.0	9.490745573990802E-5	0.0	0.19095380094869494	0.0
29	0.0	9.490745573990802E-5	0.0	0.3624515734707087	0.0
30	0.0	9.490745573990802E-5	0.0	0.5826368707872953	0.0
31	0.0	9.490745573990802E-5	0.0	1.1387945614231563	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGTGC	30	3.5992765E-4	30.833334	24
GACGTGT	25	0.005496139	29.599998	6
GGTATCA	860	0.0	24.73837	1
ATACACA	275	0.0	22.872726	37
GTCTAGA	350	0.0	22.728573	1
AGTGCGC	475	0.0	21.031578	29
GTGCGCT	460	0.0	20.913044	30
TACCGTC	180	0.0	20.555555	7
ATACCGT	195	0.0	19.923077	6
AAGTGCG	505	0.0	19.782177	28
TAGAACT	395	0.0	19.670885	4
ACTGATC	265	0.0	19.54717	8
CTAGACA	360	0.0	19.527777	4
GTTCTAG	370	0.0	19.500002	1
AAGACGG	210	0.0	19.38095	5
TCTAGAC	345	0.0	19.304346	3
ACCGTCG	195	0.0	18.97436	8
ACATGTA	245	0.0	18.87755	8
TTCAAGT	560	0.0	18.5	25
ACGGACC	220	0.0	18.5	8
>>END_MODULE