FastQCFastQC Report
Fri 10 Feb 2017
ERR1631557.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631557.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617008
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56460.3491633931310173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41680.25776001108219626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40460.250215212293322No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA20290.12547866182480236No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA17800.11007985118193601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36200.021.5662981
ATACACA11200.021.30803537
TATACAC21050.019.0712637
GCAGTCG19700.018.0304589
CAGTCGG19850.017.89420710
AGTCGGT20000.017.667511
CGGACCA2450.017.3673469
TCGGTGA21000.017.09047713
GACGTTA650.001580309517.07692315
CGGTGAT21600.016.87268414
ATACCGT2800.016.5178576
CGCCTTA13900.016.37050425
GGGCAGT22150.016.3702037
GTCGGTG22150.016.20316112
GATACAC1606.2937033E-1016.18753
ACGTTAC803.3836302E-416.187516
GTGATTC21900.016.13470516
GCCTTAT14450.016.0034626
GGTGATT22450.015.98663615
CTTATAC62150.015.86564637
Produced by FastQC (version 0.11.5)