##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631556.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3769
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.120986999204035	33.0	31.0	34.0	30.0	34.0
2	32.20084903157336	34.0	31.0	34.0	30.0	34.0
3	32.321305386044045	34.0	31.0	34.0	30.0	34.0
4	35.797028389493235	37.0	35.0	37.0	35.0	37.0
5	35.70655346245689	37.0	35.0	37.0	33.0	37.0
6	35.79755903422659	37.0	35.0	37.0	33.0	37.0
7	35.818254178827274	37.0	35.0	37.0	35.0	37.0
8	35.80021225789334	37.0	35.0	37.0	35.0	37.0
9	37.489254444149644	39.0	37.0	39.0	35.0	39.0
10	37.430087556381004	39.0	37.0	39.0	34.0	39.0
11	37.526664897850885	39.0	37.0	39.0	35.0	39.0
12	37.49376492438313	39.0	37.0	39.0	35.0	39.0
13	37.48872379941629	39.0	37.0	39.0	35.0	39.0
14	38.764924383125496	40.0	38.0	41.0	35.0	41.0
15	38.70841071902361	40.0	38.0	41.0	34.0	41.0
16	38.65879543645529	40.0	38.0	41.0	34.0	41.0
17	38.659326081188645	40.0	38.0	41.0	34.0	41.0
18	38.67763332448926	40.0	38.0	41.0	34.0	41.0
19	38.59617935791987	40.0	38.0	41.0	34.0	41.0
20	38.561156805518706	40.0	38.0	41.0	34.0	41.0
21	38.530644733351025	40.0	38.0	41.0	34.0	41.0
22	38.47704961528257	40.0	38.0	41.0	34.0	41.0
23	38.369594056778986	40.0	38.0	41.0	34.0	41.0
24	38.39665693817989	40.0	38.0	41.0	34.0	41.0
25	38.430618201114356	40.0	38.0	41.0	34.0	41.0
26	38.286813478376224	40.0	38.0	41.0	33.0	41.0
27	38.14672326877156	40.0	38.0	41.0	33.0	41.0
28	38.03422658530114	40.0	38.0	41.0	33.0	41.0
29	38.04828867073494	40.0	37.0	41.0	33.0	41.0
30	37.95914035553197	40.0	37.0	41.0	33.0	41.0
31	37.82143804722738	40.0	37.0	41.0	32.0	41.0
32	37.754046166091804	40.0	37.0	41.0	32.0	41.0
33	37.84770496152826	40.0	37.0	41.0	33.0	41.0
34	37.60679225258689	40.0	37.0	41.0	32.0	41.0
35	37.480233483682674	40.0	37.0	41.0	32.0	41.0
36	37.3879013000796	40.0	36.0	41.0	32.0	41.0
37	37.42239320774741	40.0	37.0	41.0	32.0	41.0
38	37.299548951976654	40.0	36.0	41.0	31.0	41.0
39	37.18652162377288	40.0	36.0	41.0	31.0	41.0
40	37.06473865746882	40.0	36.0	41.0	31.0	41.0
41	37.015123374900504	40.0	35.0	41.0	31.0	41.0
42	36.831254974794376	40.0	35.0	41.0	31.0	41.0
43	35.98567259219952	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	0.0
18	0.0
19	0.0
20	2.0
21	0.0
22	2.0
23	6.0
24	7.0
25	14.0
26	16.0
27	31.0
28	33.0
29	53.0
30	57.0
31	82.0
32	104.0
33	105.0
34	145.0
35	183.0
36	267.0
37	454.0
38	802.0
39	1404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.539400371451315	16.662244627222076	13.637569647121254	26.160785354205355
2	18.97054921729902	20.589015654019637	34.6511010878217	25.789334040859647
3	21.146192624038207	21.80949854072698	28.49562218094985	28.548686654284953
4	15.070310427169009	16.07853542053595	33.4040859644468	35.44706818784824
5	16.29079331387636	34.438843194481294	32.42239320774741	16.847970283894934
6	35.23481029450783	32.39586097107986	16.29079331387636	16.07853542053595
7	30.273282037675774	29.76916954099231	20.137967630671266	19.819580790660652
8	26.90368798089679	32.210135314407005	19.580790660652696	21.305386044043512
9	26.213849827540464	15.362165030512074	19.262403820642078	39.16158132130539
10	18.30724330061024	25.524011674184134	30.140620854338017	26.028124170867606
11	36.50835765455028	20.350225524011677	21.72990183072433	21.411514990713716
12	23.95860971079862	24.807641284160255	26.797559034226587	24.43618997081454
13	31.679490581055987	18.413372247280446	24.70151233749005	25.205624834173523
14	23.189174847439638	19.76651631732555	24.754576810825153	32.28973202440966
15	25.444414964181483	26.001591934200054	22.658530114088617	25.89546298752985
16	26.213849827540464	25.232157070841073	22.844255770761475	25.709737330856992
17	24.303528787476782	23.69328734412311	25.65667285752189	26.346511010878217
18	25.152560360838415	22.393207747413108	26.771026797559035	25.683205094189436
19	26.054656407535155	24.117803130803928	25.205624834173523	24.621915627487397
20	25.41788272751393	23.587158397452907	26.479172194215973	24.515786680817193
21	27.301671530910056	24.621915627487397	24.46272220748209	23.613690634120456
22	26.505704430883526	24.542318917484742	24.19739984080658	24.754576810825153
23	24.144335367471477	24.117803130803928	25.921995224197396	25.815866277527196
24	26.001591934200054	23.560626160785354	24.568851154152295	25.868930750862297
25	24.96683470416556	24.64844786415495	25.84239851419475	24.542318917484742
26	25.921995224197396	23.87901300079597	25.232157070841073	24.96683470416556
27	25.868930750862297	24.144335367471477	24.19739984080658	25.789334040859647
28	23.5340939241178	23.799416290793314	26.66489785088883	26.001591934200054
29	25.099495887503316	24.276996550809233	25.099495887503316	25.524011674184134
30	23.71981958079066	24.807641284160255	25.65667285752189	25.815866277527196
31	24.515786680817193	24.091270894136375	25.709737330856992	25.683205094189436
32	25.65667285752189	24.25046431414168	25.17909259750597	24.91377023083046
33	24.621915627487397	23.321836030777394	25.975059697532505	26.081188644202708
34	25.072963650835767	23.587158397452907	26.266914300875566	25.072963650835767
35	24.88723799416291	23.69328734412311	26.346511010878217	25.072963650835767
36	23.852480764128416	22.897320244096576	26.346511010878217	26.90368798089679
37	23.109578137436987	23.321836030777394	27.195542584239853	26.37304324754577
38	23.746351817458212	22.552401167418413	28.33642876094455	25.36481825417883
39	24.754576810825153	22.180949854072697	27.222074820907405	25.84239851419475
40	23.29530379410984	23.05651366410188	27.407800477580256	26.240382064208013
41	21.836030777394537	23.77288405412576	29.185460334306185	25.205624834173523
42	23.16264261077209	23.348368267444947	28.707880074290266	24.781109047492702
43	22.578933404085966	23.083045900769434	27.460864950915365	26.877155744229235
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	1.5
4	3.0
5	3.0
6	1.5
7	0.0
8	2.0
9	4.0
10	6.5
11	9.0
12	9.0
13	14.5
14	20.0
15	22.5
16	25.0
17	25.5
18	26.0
19	26.0
20	22.0
21	18.0
22	19.0
23	20.0
24	29.0
25	38.0
26	38.0
27	40.5
28	43.0
29	42.5
30	42.0
31	55.0
32	68.0
33	68.0
34	89.0
35	110.0
36	115.5
37	121.0
38	140.0
39	159.0
40	159.0
41	173.5
42	188.0
43	206.5
44	225.0
45	243.5
46	262.0
47	262.0
48	286.0
49	310.0
50	303.5
51	297.0
52	315.0
53	333.0
54	333.0
55	304.5
56	276.0
57	260.0
58	244.0
59	234.0
60	224.0
61	224.0
62	203.5
63	183.0
64	170.5
65	158.0
66	150.0
67	142.0
68	142.0
69	108.5
70	75.0
71	67.0
72	59.0
73	45.0
74	31.0
75	31.0
76	21.0
77	11.0
78	12.5
79	14.0
80	12.5
81	11.0
82	11.0
83	8.0
84	5.0
85	4.0
86	3.0
87	4.5
88	6.0
89	6.0
90	5.0
91	4.0
92	2.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3769.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.07508622976917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.18244013683011	88.59113823295304
2	3.620296465222349	6.739188113557973
3	0.7696693272519954	2.149111170071637
4	0.14253135689851767	0.5306447333510215
5	0.08551881413911061	0.39798355001326613
6	0.05701254275940707	0.3183868400106129
7	0.05701254275940707	0.371451313345715
8	0.028506271379703536	0.21225789334040857
9	0.0	0.0
>10	0.05701254275940707	0.689838153356328
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15	0.39798355001326613	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.29185460334306185	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	8	0.21225789334040857	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.1857256566728575	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.1857256566728575	No Hit
GGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	6	0.15919342000530645	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	6	0.15919342000530645	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	5	0.13266118333775537	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	5	0.13266118333775537	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	5	0.13266118333775537	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	4	0.10612894667020428	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	4	0.10612894667020428	No Hit
CTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCT	4	0.10612894667020428	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	4	0.10612894667020428	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	4	0.10612894667020428	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.026532236667551074	0.0
27	0.0	0.0	0.0	0.15919342000530645	0.0
28	0.0	0.0	0.0	0.23879013000795968	0.0
29	0.0	0.0	0.0	0.45104802334836824	0.0
30	0.0	0.0	0.0	0.7959671000265323	0.0
31	0.0	0.0	0.0	1.3531440700451047	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE