FastQCFastQC Report
Fri 10 Feb 2017
ERR1631555.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631555.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1263492
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42620.3373191124280961No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35760.28302513985050953No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32000.2532663443852434No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA16470.13035302162578No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14720.11650251841721197No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC14140.11191206592522944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18750.022.3973351
GTCTAGA2550.019.5882361
TCTAGAC2350.018.1063823
CAGTCGG5600.017.83928710
AGTCGGT5400.017.81481411
GTTCTAG2450.017.3673481
GCAGTCG5650.017.3539839
TAACGAA2250.017.26666613
TCCGATA2400.016.9583328
CGAACGA2300.016.89130416
AACGGAG2000.016.6525
GTGTAAC1005.878719E-616.651
ATAACGA2350.016.53191412
GTCCTAT2950.016.3050861
ACGGAGT1951.8189894E-1216.12820626
TAATAGA1059.344483E-615.8571444
TATACAC7450.015.64429537
CCGATAA2500.015.549
CTATACT1557.208655E-915.51612954
GCGTTAT2750.015.4727281
Produced by FastQC (version 0.11.5)