##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631555.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1263492
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.056123030458444	34.0	33.0	34.0	31.0	34.0
2	33.20303413080573	34.0	33.0	34.0	31.0	34.0
3	33.288430793388486	34.0	34.0	34.0	31.0	34.0
4	36.51409110623573	37.0	37.0	37.0	35.0	37.0
5	36.49928847986374	37.0	37.0	37.0	35.0	37.0
6	36.546330328961325	37.0	37.0	37.0	35.0	37.0
7	36.55615627166615	37.0	37.0	37.0	35.0	37.0
8	36.558515605955556	37.0	37.0	37.0	35.0	37.0
9	38.40377778410944	39.0	39.0	39.0	37.0	39.0
10	38.373361287606095	39.0	39.0	39.0	37.0	39.0
11	38.421762068932765	39.0	39.0	39.0	37.0	39.0
12	38.388050735580435	39.0	39.0	39.0	37.0	39.0
13	38.41224637749982	39.0	39.0	39.0	37.0	39.0
14	39.96420396805045	41.0	40.0	41.0	38.0	41.0
15	39.9531085277944	41.0	40.0	41.0	38.0	41.0
16	39.92650052394475	41.0	40.0	41.0	38.0	41.0
17	39.920142747243354	41.0	40.0	41.0	38.0	41.0
18	39.90455499520377	41.0	40.0	41.0	38.0	41.0
19	39.91687956868741	41.0	40.0	41.0	38.0	41.0
20	39.88901552206108	41.0	40.0	41.0	38.0	41.0
21	39.85806479186255	41.0	40.0	41.0	38.0	41.0
22	39.80145976389245	41.0	40.0	41.0	38.0	41.0
23	39.75209736191444	41.0	40.0	41.0	38.0	41.0
24	39.7272535164449	41.0	40.0	41.0	37.0	41.0
25	39.72159538801987	41.0	40.0	41.0	37.0	41.0
26	39.63202299658407	41.0	40.0	41.0	37.0	41.0
27	39.582438195097396	41.0	40.0	41.0	37.0	41.0
28	39.51838080494376	41.0	40.0	41.0	37.0	41.0
29	39.466871970697085	41.0	40.0	41.0	36.0	41.0
30	39.40333377654944	41.0	39.0	41.0	36.0	41.0
31	39.348444628062545	41.0	39.0	41.0	36.0	41.0
32	39.27721030287489	41.0	39.0	41.0	35.0	41.0
33	39.200560826661345	41.0	39.0	41.0	35.0	41.0
34	39.14377851225018	41.0	39.0	41.0	35.0	41.0
35	39.06111870910144	41.0	39.0	41.0	35.0	41.0
36	38.9956295726447	41.0	39.0	41.0	35.0	41.0
37	38.929034770303254	41.0	39.0	41.0	35.0	41.0
38	38.84802198985035	41.0	39.0	41.0	35.0	41.0
39	38.756098970155726	40.0	38.0	41.0	35.0	41.0
40	38.661496075954574	40.0	38.0	41.0	35.0	41.0
41	38.56479661129631	40.0	38.0	41.0	35.0	41.0
42	38.42753891595673	40.0	38.0	41.0	35.0	41.0
43	37.651617897066224	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	1.0
13	0.0
14	2.0
15	1.0
16	5.0
17	8.0
18	20.0
19	38.0
20	67.0
21	111.0
22	198.0
23	400.0
24	690.0
25	1017.0
26	1529.0
27	2276.0
28	3330.0
29	4597.0
30	6487.0
31	8271.0
32	10695.0
33	13863.0
34	20936.0
35	31359.0
36	51549.0
37	102477.0
38	231644.0
39	771916.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.34772202752372	17.74431496202588	12.472734295112275	26.435228715338127
2	19.09509518065805	19.689083903974065	33.84746401243538	27.368356902932504
3	20.044843972102715	21.147581464702586	28.97533185805688	29.832242705137823
4	14.744533404247909	15.318814840141448	33.339031826082	36.59761992952864
5	16.366071174174433	34.81953190047899	32.08971643666917	16.724680488677414
6	34.918147483324	33.664320787151794	15.809280945189997	15.60825078433421
7	30.682188727748176	29.65258189208954	19.99791055265882	19.667318827503458
8	28.749370791425665	31.399328211021516	19.311242176444328	20.540058821108484
9	27.33210815739237	14.181253225188604	19.232967046882766	39.253671570536255
10	18.903087633320986	25.112940960449293	30.52991233818655	25.454059068043165
11	37.38401192884482	21.023401810221195	20.16285025943971	21.42973600149427
12	23.50272103028749	24.73961053967892	27.21805915668639	24.5396092733472
13	31.248001570251333	18.181832571951386	24.41353012128292	26.15663573651436
14	23.970472310073983	19.682831391097054	23.747597927014972	32.59909837181399
15	26.959964922611302	25.99715708528428	21.129694529130376	25.913183462974043
16	26.30566715103855	24.765095465582686	23.08926372307858	25.839973660300185
17	25.135893222909207	25.249863077882566	24.048351710972447	25.565891988235776
18	25.683185963979195	23.102876789089287	25.439812836171498	25.77412441076002
19	25.62358922731604	24.3804472050476	24.868143209454434	25.12782035818193
20	26.041795278482176	23.40901248286495	24.901938437283338	25.647253801369533
21	27.390359416600973	23.646212243528254	24.4751846469942	24.48824369287657
22	27.115644578675607	23.242964735827375	24.065526334951073	25.575864350545945
23	25.876301551572944	23.327650669731188	24.710326618609376	26.08572116008649
24	25.28468720023554	24.125123071614222	24.70280777401044	25.887381954139798
25	25.776736219936492	23.438612986864975	24.612106764427473	26.172544028771057
26	25.85762315867453	24.180920813111598	24.595644452042436	25.36581157617144
27	25.962016380000826	23.499555200982673	24.496870577732192	26.041557841284313
28	25.059834173861013	23.62373485546406	25.403089216235642	25.913341754439283
29	24.651758776470288	23.946253715892148	25.52972238842826	25.872265119209302
30	24.31839695067321	24.590974853817833	25.72149249856746	25.36913569694149
31	25.642663348877555	24.05262558053395	24.373324089111765	25.931386981476734
32	24.965492460577508	23.716177071164676	25.040285177903776	26.278045290354036
33	24.571663295058457	23.22571096611613	25.875193511316258	26.327432227509156
34	25.675746265112874	23.0974948792711	25.343017605176765	25.883741250439257
35	25.15496734447072	23.229430815549286	26.11999126231112	25.495610577668877
36	24.26861428485499	23.522507463442587	26.025174674631895	26.18370357707053
37	24.771189686994457	22.62507400126	26.428659619530638	26.175076692214912
38	23.892513763442903	22.300576497516406	27.361075495531434	26.44583424350926
39	24.336442177710662	22.100100356788964	27.442991328793536	26.120466136706845
40	23.642334102629857	22.56254887248989	27.84544737916821	25.949669645712042
41	22.940153162821765	22.536984800853507	28.526812991297135	25.99604904502759
42	23.383210974030703	22.716012448040825	28.48304540115806	25.41773117677041
43	21.99950613062845	22.171331516147312	28.66017355076249	27.168988802461747
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	214.0
1	193.0
2	172.0
3	468.0
4	764.0
5	764.0
6	939.0
7	1114.0
8	1076.0
9	1038.0
10	1541.5
11	2045.0
12	2045.0
13	3289.5
14	4534.0
15	6608.0
16	8682.0
17	8197.0
18	7712.0
19	7712.0
20	8872.0
21	10032.0
22	8859.0
23	7686.0
24	8671.0
25	9656.0
26	9656.0
27	11103.0
28	12550.0
29	14977.0
30	17404.0
31	20011.0
32	22618.0
33	22618.0
34	27342.5
35	32067.0
36	37767.5
37	43468.0
38	48048.0
39	52628.0
40	52628.0
41	57621.5
42	62615.0
43	67007.0
44	71399.0
45	76622.5
46	81846.0
47	81846.0
48	87460.5
49	93075.0
50	94866.0
51	96657.0
52	96830.5
53	97004.0
54	97004.0
55	93463.5
56	89923.0
57	87626.5
58	85330.0
59	82558.5
60	79787.0
61	79787.0
62	75149.5
63	70512.0
64	65328.0
65	60144.0
66	53738.0
67	47332.0
68	47332.0
69	40082.0
70	32832.0
71	28263.5
72	23695.0
73	18310.0
74	12925.0
75	12925.0
76	10467.0
77	8009.0
78	6628.0
79	5247.0
80	4672.5
81	4098.0
82	4098.0
83	3315.5
84	2533.0
85	2254.5
86	1976.0
87	1653.5
88	1331.0
89	1331.0
90	989.5
91	648.0
92	388.0
93	128.0
94	83.0
95	38.0
96	38.0
97	29.0
98	20.0
99	12.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1263492.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.77174834908016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.68394882029001	50.07883277150339
2	7.17503155938683	8.290282352912085
3	2.1322115647840136	3.695447698431015
4	1.0662127211491959	2.463878920512773
5	0.6375238168921626	1.841543275801954
6	0.42493532497238984	1.4729553995363704
7	0.29023685507987496	1.173724338731209
8	0.21207228527555322	0.9801429357402879
9	0.16522791275289297	0.8590954856223515
>10	1.0149855197277768	11.189279620929165
>50	0.10606266043824585	4.208187512587146
>100	0.08276646739489446	9.402310018641689
>500	0.007549172688878865	2.8437983479568354
>1k	0.0012353191672710869	1.5005213210938069
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4262	0.3373191124280961	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3576	0.28302513985050953	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3200	0.2532663443852434	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1647	0.13035302162578	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1472	0.11650251841721197	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1414	0.11191206592522944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.914573262038858E-5	0.0
3	0.0	0.0	0.0	7.914573262038858E-5	0.0
4	0.0	0.0	0.0	7.914573262038858E-5	0.0
5	0.0	7.914573262038858E-5	0.0	7.914573262038858E-5	0.0
6	0.0	7.914573262038858E-5	0.0	7.914573262038858E-5	0.0
7	0.0	7.914573262038858E-5	0.0	7.914573262038858E-5	0.0
8	0.0	7.914573262038858E-5	0.0	7.914573262038858E-5	0.0
9	0.0	7.914573262038858E-5	0.0	7.914573262038858E-5	0.0
10	0.0	7.914573262038858E-5	0.0	3.9572866310194285E-4	0.0
11	0.0	7.914573262038858E-5	0.0	7.123115935834972E-4	0.0
12	0.0	7.914573262038858E-5	0.0	0.0011871859893058286	0.0
13	0.0	7.914573262038858E-5	0.0	0.0013454774545466057	0.0
14	0.0	7.914573262038858E-5	0.0	0.0018203518502689373	0.0
15	0.0	7.914573262038858E-5	0.0	0.0025326634438524345	0.0
16	0.0	7.914573262038858E-5	0.0	0.004036432363639817	0.0
17	0.0	7.914573262038858E-5	0.0	0.005698492748667978	0.0
18	0.0	7.914573262038858E-5	0.0	0.006964824470594194	0.0
19	0.0	7.914573262038858E-5	0.0	0.007835427529418468	0.0
20	0.0	7.914573262038858E-5	0.0	0.009576633647067017	0.0
21	0.0	7.914573262038858E-5	0.0	0.01163442269519712	0.0
22	0.0	7.914573262038858E-5	0.0	0.014958543465253441	0.0
23	0.0	7.914573262038858E-5	0.0	0.020023870352958308	0.0
24	0.0	7.914573262038858E-5	0.0	0.030154524128368047	0.0
25	0.0	7.914573262038858E-5	0.0	0.035061559550832136	0.0
26	0.0	7.914573262038858E-5	0.0	0.04725000237437198	0.0
27	0.0	7.914573262038858E-5	0.0	0.09267965289847502	0.0
28	0.0	7.914573262038858E-5	0.0	0.25120855533711334	0.0
29	0.0	7.914573262038858E-5	0.0	0.4836595720431946	0.0
30	0.0	7.914573262038858E-5	0.0	0.8039623519579071	0.0
31	0.0	7.914573262038858E-5	0.0	1.4615842443007159	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1875	0.0	22.397335	1
GTCTAGA	255	0.0	19.588236	1
TCTAGAC	235	0.0	18.106382	3
CAGTCGG	560	0.0	17.839287	10
AGTCGGT	540	0.0	17.814814	11
GTTCTAG	245	0.0	17.367348	1
GCAGTCG	565	0.0	17.353983	9
TAACGAA	225	0.0	17.266666	13
TCCGATA	240	0.0	16.958332	8
CGAACGA	230	0.0	16.891304	16
AACGGAG	200	0.0	16.65	25
GTGTAAC	100	5.878719E-6	16.65	1
ATAACGA	235	0.0	16.531914	12
GTCCTAT	295	0.0	16.305086	1
ACGGAGT	195	1.8189894E-12	16.128206	26
TAATAGA	105	9.344483E-6	15.857144	4
TATACAC	745	0.0	15.644295	37
CCGATAA	250	0.0	15.54	9
CTATACT	155	7.208655E-9	15.5161295	4
GCGTTAT	275	0.0	15.472728	1
>>END_MODULE