FastQCFastQC Report
Fri 10 Feb 2017
ERR1631554.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631554.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548248
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9820.17911602048707884No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC8470.15449212765026046No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC7890.14391297369073852No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC7890.14391297369073852No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT6750.12311946418409186No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT6460.11782988720433088No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG6040.1101691205439874No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC5980.10907472530679546No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC5800.10579153959521968No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC5710.10414994673943179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG556.251703E-726.909091
GGTATCA3200.026.5937521
TACACCG358.863455E-426.428575
GTACACT400.001929905423.1250024
GTCTATA400.001929905423.1250021
TTAGTAG400.001929905423.1250023
AACTAGC400.001929905423.12500237
TTCGCGA400.001929905423.12500233
TGTACGC400.001929905423.1250022
TTACACC400.001929905423.1250024
TCGCGAT400.001929905423.12500234
GGCGTAC450.00382329420.5555551
GATTCGG450.00382329420.55555522
TAGTAGA555.138379E-420.1818184
TAGAACT1052.2530003E-819.380954
ATACACA1750.019.02857237
CTAGAGT1850.019.04
GACCGTG801.6141576E-518.57
AGACCGT1002.8681097E-718.56
CGTGTCA500.00703030218.510