##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631554.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	548248
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02992258977689	34.0	33.0	34.0	31.0	34.0
2	33.17513424581576	34.0	33.0	34.0	31.0	34.0
3	33.26469043206724	34.0	34.0	34.0	31.0	34.0
4	36.50367899198903	37.0	37.0	37.0	35.0	37.0
5	36.489442733945225	37.0	37.0	37.0	35.0	37.0
6	36.53813785002408	37.0	37.0	37.0	35.0	37.0
7	36.53987611445915	37.0	37.0	37.0	35.0	37.0
8	36.535958179510004	37.0	37.0	37.0	35.0	37.0
9	38.382589630969925	39.0	39.0	39.0	37.0	39.0
10	38.34238519793962	39.0	39.0	39.0	37.0	39.0
11	38.39909128715472	39.0	39.0	39.0	37.0	39.0
12	38.35210889962207	39.0	39.0	39.0	37.0	39.0
13	38.38587099268944	39.0	39.0	39.0	37.0	39.0
14	39.921446133866425	41.0	40.0	41.0	38.0	41.0
15	39.92032620274036	41.0	40.0	41.0	38.0	41.0
16	39.89974427631291	41.0	40.0	41.0	38.0	41.0
17	39.887326538354905	41.0	40.0	41.0	38.0	41.0
18	39.85840714421211	41.0	40.0	41.0	38.0	41.0
19	39.86882213888605	41.0	40.0	41.0	38.0	41.0
20	39.84441712509667	41.0	40.0	41.0	38.0	41.0
21	39.80972479607769	41.0	40.0	41.0	38.0	41.0
22	39.76156228568093	41.0	40.0	41.0	38.0	41.0
23	39.697666019757484	41.0	40.0	41.0	37.0	41.0
24	39.674773095387486	41.0	40.0	41.0	37.0	41.0
25	39.65820759948053	41.0	40.0	41.0	37.0	41.0
26	39.58534641257241	41.0	40.0	41.0	37.0	41.0
27	39.53389706847996	41.0	40.0	41.0	37.0	41.0
28	39.44237644277772	41.0	39.0	41.0	37.0	41.0
29	39.4024656724694	41.0	39.0	41.0	36.0	41.0
30	39.34615356553968	41.0	39.0	41.0	36.0	41.0
31	39.31476448614496	41.0	39.0	41.0	36.0	41.0
32	39.24372546730677	41.0	39.0	41.0	35.0	41.0
33	39.1719513796676	41.0	39.0	41.0	35.0	41.0
34	39.12658140111774	41.0	39.0	41.0	35.0	41.0
35	39.029194816944155	41.0	39.0	41.0	35.0	41.0
36	38.98211758182428	40.0	39.0	41.0	35.0	41.0
37	38.92369146809473	40.0	39.0	41.0	35.0	41.0
38	38.853921947731685	40.0	38.0	41.0	35.0	41.0
39	38.78229560344953	40.0	38.0	41.0	35.0	41.0
40	38.69656068056792	40.0	38.0	41.0	35.0	41.0
41	38.61456494141338	40.0	38.0	41.0	35.0	41.0
42	38.49677700602647	40.0	38.0	41.0	35.0	41.0
43	37.68236272635742	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	2.0
17	2.0
18	3.0
19	12.0
20	30.0
21	48.0
22	100.0
23	170.0
24	275.0
25	440.0
26	635.0
27	941.0
28	1323.0
29	1820.0
30	2475.0
31	3284.0
32	4263.0
33	5948.0
34	9157.0
35	14118.0
36	24172.0
37	45420.0
38	112733.0
39	320876.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8421079511462	18.008638426405568	12.190650946287082	26.958602676161153
2	19.97435467160847	19.390677211772775	32.99619880054282	27.638769316075933
3	20.28352132611519	20.661634880565	28.265675387780714	30.789168405539098
4	15.09371671214487	14.852949759962645	32.81598838481855	37.23734514307394
5	17.12564386919788	33.87135019188396	31.836139849119377	17.166866089798777
6	36.36310574776378	33.16272927580219	15.017473843953832	15.456691132480191
7	31.555609869985844	28.76599641038362	19.71844858531176	19.959945134318776
8	28.63740497001357	31.11912856955247	19.165231792911236	21.07823466752273
9	27.468408457486394	13.98855992178722	18.87065707490041	39.67237454582598
10	19.267010549970088	24.083808787264157	29.866593220586303	26.78258744217945
11	39.18628066130656	20.101304519122735	19.700573463104288	21.01184135646642
12	24.355401205293955	23.712626402649896	26.325130233033228	25.606842159022925
13	31.44489355182326	17.191854781047994	23.833374677153405	27.52987698997534
14	24.532693233719048	19.225058732544394	22.26382950781398	33.97841852592258
15	27.483912390013277	25.5287752987699	20.007551327136625	26.979760984080198
16	27.821168522274593	23.952663758007326	21.579102887744234	26.64706483197385
17	26.643416847849878	24.176285184806876	22.413396856896878	26.766901110446366
18	26.928506807138376	22.08398389050211	24.095847134873267	26.891662167486245
19	26.756869154105438	23.307152967270287	23.033918956384703	26.90205892223957
20	27.163254585516043	22.286629408588816	23.023522201631376	27.526593804263765
21	29.106170929944113	22.8444061811443	22.705053187608527	25.34436970130306
22	28.460112941588477	22.272584669711517	22.345909150603376	26.92139323809663
23	26.663480760531733	22.14837081029023	23.417139688608074	27.771008740569965
24	26.407757073441218	23.42297646320643	22.90952269775722	27.259743765595136
25	26.99873050152486	22.445499117187843	22.9392537683676	27.6165166129197
26	26.896404546847414	23.70405363995856	23.187134289591572	26.212407523602455
27	27.659562825582583	22.29957975222892	22.918277859654754	27.122579562533744
28	26.285549605288118	22.575184952795084	23.930228655644893	27.209036786271906
29	25.704060935926808	23.109797026163342	23.96543170244123	27.220710335468617
30	25.174556040332114	23.845413024762514	24.30141104025915	26.678619894646218
31	27.08135734193285	23.105419445214576	22.481614160015177	27.3316090528374
32	26.005019626154585	22.563329004392173	23.105237046008376	28.326414323444865
33	25.43757569567057	22.157125972187767	24.105514292801796	28.29978403933986
34	26.90479491033255	22.030358523879702	24.07505362536662	26.989792940421125
35	26.725496490639273	21.778100421706963	25.083903634851378	26.41249945280238
36	25.1896951744466	22.50167807269703	24.923757131808962	27.384869621047407
37	26.04624184675548	21.30422728400286	25.448702047248688	27.200828821992967
38	24.81851278983234	20.721461820198158	26.69339423034831	27.76663115962119
39	25.316097824342272	20.42962309028031	26.77875705884928	27.475522026528143
40	24.594161766207996	21.225978024543636	27.2909340298551	26.88892617939327
41	23.54263034247275	21.307328070508238	28.533802220892735	26.616239366126276
42	24.419605725875883	21.48990967591309	28.161160642628886	25.929323955582145
43	22.580292130568647	20.709788271001443	28.42308590273015	28.28683369569976
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	21.0
2	24.0
3	52.5
4	81.0
5	81.0
6	91.0
7	101.0
8	98.5
9	96.0
10	142.0
11	188.0
12	188.0
13	330.5
14	473.0
15	709.0
16	945.0
17	949.0
18	953.0
19	953.0
20	1114.0
21	1275.0
22	1293.0
23	1311.0
24	1646.0
25	1981.0
26	1981.0
27	2431.0
28	2881.0
29	3679.0
30	4477.0
31	5516.5
32	6556.0
33	6556.0
34	8630.5
35	10705.0
36	13512.0
37	16319.0
38	19293.0
39	22267.0
40	22267.0
41	24216.0
42	26165.0
43	28363.5
44	30562.0
45	33161.5
46	35761.0
47	35761.0
48	38251.0
49	40741.0
50	42050.5
51	43360.0
52	45005.5
53	46651.0
54	46651.0
55	45546.5
56	44442.0
57	43105.5
58	41769.0
59	40634.0
60	39499.0
61	39499.0
62	36927.0
63	34355.0
64	31728.5
65	29102.0
66	25770.5
67	22439.0
68	22439.0
69	18904.0
70	15369.0
71	13287.5
72	11206.0
73	8405.5
74	5605.0
75	5605.0
76	4399.0
77	3193.0
78	2714.0
79	2235.0
80	2059.5
81	1884.0
82	1884.0
83	1507.0
84	1130.0
85	1065.0
86	1000.0
87	839.0
88	678.0
89	678.0
90	510.5
91	343.0
92	211.5
93	80.0
94	49.0
95	18.0
96	18.0
97	14.0
98	10.0
99	5.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	548248.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.61275868056309
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.57837975639954	47.28284160590396
2	6.873841275671196	7.5079886959344995
3	2.1906863763175326	3.589182792438799
4	1.1609499167744575	2.53610710580093
5	0.6460722709485819	1.7641894511759133
6	0.45960554503660683	1.5060196031599726
7	0.2797974524331086	1.069635752441598
8	0.22259795866583829	0.9725350879522707
9	0.17663034020679286	0.8681643130841403
>10	1.1425490519659076	12.308505602838988
>50	0.1374624974969475	5.224649307757717
>100	0.12673371672336756	13.688749600917028
>500	0.004693841360124724	1.6814310805941823
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	982	0.17911602048707884	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	847	0.15449212765026046	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	789	0.14391297369073852	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	789	0.14391297369073852	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	675	0.12311946418409186	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	646	0.11782988720433088	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	604	0.1101691205439874	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	598	0.10907472530679546	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	580	0.10579153959521968	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	571	0.10414994673943179	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.823992061986546E-4	0.0
11	0.0	1.823992061986546E-4	0.0	1.823992061986546E-4	0.0
12	0.0	1.823992061986546E-4	0.0	1.823992061986546E-4	0.0
13	0.0	1.823992061986546E-4	0.0	1.823992061986546E-4	0.0
14	0.0	1.823992061986546E-4	0.0	1.823992061986546E-4	0.0
15	0.0	1.823992061986546E-4	0.0	0.0012767944433905824	0.0
16	0.0	1.823992061986546E-4	0.0	0.001459193649589237	0.0
17	0.0	1.823992061986546E-4	0.0	0.002553588886781165	0.0
18	0.0	1.823992061986546E-4	0.0	0.0031007865053771285	0.0
19	0.0	1.823992061986546E-4	0.0	0.003283185711575783	0.0
20	0.0	1.823992061986546E-4	0.0	0.0040127825363704015	0.0
21	0.0	1.823992061986546E-4	0.0	0.00474237936116502	0.0
22	0.0	1.823992061986546E-4	0.0	0.006748770629350221	0.0
23	0.0	3.647984123973092E-4	0.0	0.008937561103734077	0.0
24	0.0	3.647984123973092E-4	0.0	0.01258554522770717	0.0
25	0.0	3.647984123973092E-4	0.0	0.014774335702091024	0.0
26	0.0	3.647984123973092E-4	0.0	0.02097590871284528	0.0
27	0.0	3.647984123973092E-4	0.0	0.04870058805504079	0.0
28	0.0	3.647984123973092E-4	0.0	0.18659438794122368	0.0
29	0.0	3.647984123973092E-4	0.0	0.3720943806452554	0.0
30	0.0	3.647984123973092E-4	0.0	0.6407684113758737	0.0
31	0.0	3.647984123973092E-4	0.0	1.3928003385329266	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTACG	55	6.251703E-7	26.90909	1
GGTATCA	320	0.0	26.593752	1
TACACCG	35	8.863455E-4	26.42857	5
GTACACT	40	0.0019299054	23.125002	4
GTCTATA	40	0.0019299054	23.125002	1
TTAGTAG	40	0.0019299054	23.125002	3
AACTAGC	40	0.0019299054	23.125002	37
TTCGCGA	40	0.0019299054	23.125002	33
TGTACGC	40	0.0019299054	23.125002	2
TTACACC	40	0.0019299054	23.125002	4
TCGCGAT	40	0.0019299054	23.125002	34
GGCGTAC	45	0.003823294	20.555555	1
GATTCGG	45	0.003823294	20.555555	22
TAGTAGA	55	5.138379E-4	20.181818	4
TAGAACT	105	2.2530003E-8	19.38095	4
ATACACA	175	0.0	19.028572	37
CTAGAGT	185	0.0	19.0	4
GACCGTG	80	1.6141576E-5	18.5	7
AGACCGT	100	2.8681097E-7	18.5	6
CGTGTCA	50	0.007030302	18.5	10
>>END_MODULE