FastQCFastQC Report
Fri 10 Feb 2017
ERR1631553.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631553.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043615
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA21010.20131945209679816No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC18860.18071798508070505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16190.15513383767002198No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15930.152642497472727No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC14920.1429645990140042No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC14700.1408565419239854No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13380.1282081993838724No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC13250.12696252928522492No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG12360.11843448014833056No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC11650.11163120499417889No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG11450.10971478945779813No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC11280.1080858362518745No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT10680.10233658964273223No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT10640.10195330653545608No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC10540.10099509876726571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACC400.001931068823.1254
GGTATCA9900.022.9848481
TCTAGAC2400.021.5833323
CTAGACA2600.020.6346154
ACGCCGT909.476207E-820.55555524
CGTGAAT909.476207E-820.55555510
TATTCGT450.00382557620.5555556
GGACCGA450.00382557620.5555556
CTAGAAC2400.019.2708323
GTTCTAG2600.019.2115381
CTTATAC7200.018.537
TGATTCG801.6164382E-518.513
CGCGCTT500.007034459618.49999812
TTGTATA500.007034459618.4999982
GGTATAC500.007034459618.4999983
GTCTAGA2350.018.1063841
TAGGACA1258.574716E-917.764
GTCAAAA2000.017.57499931
TTGCGTT752.0670361E-417.2666664
TAGAACT2800.017.1785724
Produced by FastQC (version 0.11.5)