##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631553.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1043615
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.088290221968826	34.0	33.0	34.0	31.0	34.0
2	33.219096122612264	34.0	33.0	34.0	31.0	34.0
3	33.30587045989182	34.0	34.0	34.0	31.0	34.0
4	36.51878710060703	37.0	37.0	37.0	35.0	37.0
5	36.50503777734126	37.0	37.0	37.0	35.0	37.0
6	36.5603186999037	37.0	37.0	37.0	35.0	37.0
7	36.568675229850086	37.0	37.0	37.0	35.0	37.0
8	36.56707981391605	37.0	37.0	37.0	35.0	37.0
9	38.422242876923	39.0	39.0	39.0	37.0	39.0
10	38.3819952760357	39.0	39.0	39.0	37.0	39.0
11	38.436336196777546	39.0	39.0	39.0	37.0	39.0
12	38.39975853164242	39.0	39.0	39.0	37.0	39.0
13	38.4217369432214	39.0	39.0	39.0	37.0	39.0
14	39.98693100424965	41.0	40.0	41.0	38.0	41.0
15	39.97883031577737	41.0	40.0	41.0	38.0	41.0
16	39.95284180468851	41.0	40.0	41.0	38.0	41.0
17	39.95050186131859	41.0	40.0	41.0	38.0	41.0
18	39.926052231905445	41.0	40.0	41.0	38.0	41.0
19	39.92924689660459	41.0	40.0	41.0	38.0	41.0
20	39.89970535111128	41.0	40.0	41.0	38.0	41.0
21	39.87335367927828	41.0	40.0	41.0	38.0	41.0
22	39.81898976155	41.0	40.0	41.0	38.0	41.0
23	39.767540711852554	41.0	40.0	41.0	38.0	41.0
24	39.73735908357009	41.0	40.0	41.0	38.0	41.0
25	39.73756605644802	41.0	40.0	41.0	37.0	41.0
26	39.657267287265896	41.0	40.0	41.0	37.0	41.0
27	39.60303464400186	41.0	40.0	41.0	37.0	41.0
28	39.54091978363669	41.0	40.0	41.0	37.0	41.0
29	39.5070940912118	41.0	40.0	41.0	37.0	41.0
30	39.446895646383005	41.0	39.0	41.0	36.0	41.0
31	39.4137301591104	41.0	39.0	41.0	36.0	41.0
32	39.337193313626194	41.0	39.0	41.0	36.0	41.0
33	39.2675814356827	41.0	39.0	41.0	35.0	41.0
34	39.219273391049384	41.0	39.0	41.0	35.0	41.0
35	39.13678703353248	41.0	39.0	41.0	35.0	41.0
36	39.07378199815066	41.0	39.0	41.0	35.0	41.0
37	39.01545589130091	41.0	39.0	41.0	35.0	41.0
38	38.94134810250907	41.0	39.0	41.0	35.0	41.0
39	38.85656012993297	40.0	38.0	41.0	35.0	41.0
40	38.770789994394484	40.0	38.0	41.0	35.0	41.0
41	38.683269213263515	40.0	38.0	41.0	35.0	41.0
42	38.540223166589215	40.0	38.0	41.0	35.0	41.0
43	37.74798464951155	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	1.0
16	4.0
17	6.0
18	10.0
19	21.0
20	34.0
21	102.0
22	159.0
23	302.0
24	515.0
25	785.0
26	1164.0
27	1731.0
28	2447.0
29	3549.0
30	4730.0
31	6290.0
32	8088.0
33	10885.0
34	16492.0
35	24680.0
36	41289.0
37	80582.0
38	197961.0
39	641786.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.75884305994069	16.805335300853283	11.011148747382896	27.424672891823132
2	20.138269380949872	19.430824585694918	32.165118362614564	28.265787670740643
3	20.982833707832867	20.361148507830954	28.02958945588172	30.62642832845446
4	14.896010501957138	14.28754856915625	32.26707166915002	38.54936925973659
5	16.78281741830081	34.3803989018939	31.783368387767517	17.053415292037773
6	36.85650359567465	32.51678061354043	15.037921072426133	15.58879471835878
7	31.945688783698966	28.517892134551538	19.20152546676696	20.334893614982537
8	29.605745413778067	30.083603627774608	18.97145978162445	21.33919117682287
9	27.874072335104422	13.196437383517868	18.168577492657732	40.76091278871998
10	18.737273803078722	24.395682315796535	30.41054411828117	26.456499762843578
11	39.020711660909434	20.124758651418386	19.440119201046365	21.414410486625815
12	24.319888081332675	23.825069589839167	26.378022546628788	25.477019782199374
13	31.668287634807857	17.38332622662572	23.77054756782913	27.177838570737293
14	24.362528327017145	18.9324607254591	22.44323816733182	34.261772780191926
15	27.424385429492677	25.435146102729455	20.16222457515463	26.978243892623237
16	27.680897649037238	23.867039090085903	21.787728233112784	26.664335027764068
17	26.630318651993313	24.323816733182255	22.473421712029822	26.572442902794613
18	27.056721108838026	21.943820278550998	24.178935718631873	26.820522893979103
19	26.6730547184546	23.158156983178664	23.355931066533156	26.812857231833583
20	26.6790914273942	22.24766796184417	23.656616664191297	27.416623946570333
21	28.15856422148014	22.944189188541753	23.530229059566988	25.36701753041112
22	28.378856187387115	22.198128620228726	22.738749443041733	26.68426574934243
23	26.572730365125068	22.292991189279572	23.496404325349864	27.637874120245492
24	26.175649065986978	23.393109527938943	23.249761645817664	27.181479760256416
25	27.066111544966294	22.368497961412974	23.32584334261198	27.239547151008754
26	26.969428381155886	23.391863857840296	23.08954930697623	26.549158454027587
27	27.637586657915037	22.136515860734082	23.43105455555928	26.794842925791603
28	26.22442184138787	22.532926414434442	24.02571829649823	27.21693344767946
29	25.703827560930037	22.869353161846085	24.110615504759895	27.316203772463982
30	25.050138221470565	23.637548329604307	24.79956689008878	26.512746558836355
31	26.99750386876386	23.149820575595406	22.944764113202666	26.907911442438063
32	26.236878542374342	22.94361426388084	23.446769162957604	27.372738030787215
33	25.862985871226456	22.47821275087077	23.967267622638616	27.691533755264153
34	27.049438729799785	22.47437991979801	23.644064142428004	26.832117207974203
35	26.523478485840084	22.56416398767745	24.658327065057517	26.25403046142495
36	25.545244175294528	23.23644255783982	24.266899191751747	26.951414075113906
37	26.73361344940424	22.08697651911864	24.648649166598794	26.53076086487833
38	25.563258481336508	21.628090818932268	25.445878029733187	27.362772669998037
39	26.378884933620157	21.63633140573871	25.137047666045433	26.847735994595705
40	25.434283715738083	22.32806159359534	25.308854318881963	26.92880037178461
41	25.1021689032833	22.176952228551716	26.340939905999818	26.379938962165166
42	25.737748115923974	22.055451483545177	26.32800410112925	25.878796299401603
43	24.22071357732497	21.311115689214894	26.386838058096135	28.081332675364003
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	88.0
1	83.5
2	79.0
3	203.0
4	327.0
5	327.0
6	377.5
7	428.0
8	384.0
9	340.0
10	521.0
11	702.0
12	702.0
13	1212.5
14	1723.0
15	2475.0
16	3227.0
17	3062.0
18	2897.0
19	2897.0
20	3427.0
21	3957.0
22	3292.5
23	2628.0
24	3030.0
25	3432.0
26	3432.0
27	4257.5
28	5083.0
29	6585.0
30	8087.0
31	9979.0
32	11871.0
33	11871.0
34	15433.0
35	18995.0
36	23453.5
37	27912.0
38	32675.0
39	37438.0
40	37438.0
41	41704.5
42	45971.0
43	49708.0
44	53445.0
45	59734.5
46	66024.0
47	66024.0
48	71714.5
49	77405.0
50	79869.5
51	82334.0
52	85069.0
53	87804.0
54	87804.0
55	86173.0
56	84542.0
57	82715.0
58	80888.0
59	78458.5
60	76029.0
61	76029.0
62	73533.0
63	71037.0
64	66346.0
65	61655.0
66	55663.0
67	49671.0
68	49671.0
69	39951.5
70	30232.0
71	25568.5
72	20905.0
73	15342.5
74	9780.0
75	9780.0
76	7566.5
77	5353.0
78	4417.5
79	3482.0
80	3114.5
81	2747.0
82	2747.0
83	2263.0
84	1779.0
85	1622.5
86	1466.0
87	1297.0
88	1128.0
89	1128.0
90	855.5
91	583.0
92	338.5
93	94.0
94	59.0
95	24.0
96	24.0
97	21.5
98	19.0
99	11.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1043615.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.90272382398189
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.06661276529243	36.468505592282824
2	7.8981013396178525	6.93496323694128
3	2.8123079923951217	3.7040394329429995
4	1.4440572291663276	2.535921828724552
5	0.9197911042200286	2.019066741216363
6	0.6284191922169882	1.655358854495533
7	0.44112929105078236	1.3556744204969997
8	0.32722458727236675	1.149284054674814
9	0.267800437153727	1.0581451769071524
>10	1.7771220897313447	15.600514705343949
>50	0.22969994473344643	7.079909938697387
>100	0.17003151703666525	14.195298386229776
>500	0.014205717991819857	4.1625635677156225
>1k	0.003496792121063349	2.0807540633308204
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2101	0.20131945209679816	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1886	0.18071798508070505	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1619	0.15513383767002198	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1593	0.152642497472727	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1492	0.1429645990140042	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1470	0.1408565419239854	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1338	0.1282081993838724	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1325	0.12696252928522492	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1236	0.11843448014833056	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1165	0.11163120499417889	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	1145	0.10971478945779813	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1128	0.1080858362518745	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1068	0.10233658964273223	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1064	0.10195330653545608	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1054	0.10099509876726571	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	9.582077681903767E-5	0.0	0.0	0.0
6	0.0	9.582077681903767E-5	0.0	0.0	0.0
7	0.0	9.582077681903767E-5	0.0	0.0	0.0
8	0.0	9.582077681903767E-5	0.0	0.0	0.0
9	0.0	9.582077681903767E-5	0.0	0.0	0.0
10	0.0	9.582077681903767E-5	0.0	1.9164155363807534E-4	0.0
11	0.0	9.582077681903767E-5	0.0	1.9164155363807534E-4	0.0
12	0.0	9.582077681903767E-5	0.0	3.832831072761507E-4	0.0
13	0.0	9.582077681903767E-5	0.0	4.7910388409518837E-4	0.0
14	0.0	9.582077681903767E-5	0.0	6.707454377332637E-4	0.0
15	0.0	9.582077681903767E-5	0.0	6.707454377332637E-4	0.0
16	0.0	1.9164155363807534E-4	0.0	0.0010540285450094144	0.0
17	0.0	1.9164155363807534E-4	0.0	0.0014373116522855651	0.0
18	0.0	1.9164155363807534E-4	0.0	0.0014373116522855651	0.0
19	0.0	1.9164155363807534E-4	0.0	0.0015331324291046027	0.0
20	0.0	1.9164155363807534E-4	0.0	0.0019164155363807535	0.0
21	0.0	2.87462330457113E-4	0.0	0.002395519420475942	0.0
22	0.0	2.87462330457113E-4	0.0	0.0027788025277520924	0.0
23	0.0	3.832831072761507E-4	0.0	0.003928651849580545	0.0
24	0.0	3.832831072761507E-4	0.0	0.006132529716418411	0.0
25	0.0	3.832831072761507E-4	0.0	0.008048945252799164	0.0
26	0.0	3.832831072761507E-4	0.0	0.013223267201027199	0.0
27	0.0	4.7910388409518837E-4	0.0	0.03162085635028243	0.0
28	0.0	5.74924660914226E-4	0.0	0.0908380964244477	0.0
29	0.0	6.707454377332637E-4	0.0	0.17554366313247702	0.0
30	0.0	6.707454377332637E-4	0.0	0.2930199355126172	0.0
31	0.0	6.707454377332637E-4	0.0	0.569750338965998	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACC	40	0.0019310688	23.125	4
GGTATCA	990	0.0	22.984848	1
TCTAGAC	240	0.0	21.583332	3
CTAGACA	260	0.0	20.634615	4
ACGCCGT	90	9.476207E-8	20.555555	24
CGTGAAT	90	9.476207E-8	20.555555	10
TATTCGT	45	0.003825576	20.555555	6
GGACCGA	45	0.003825576	20.555555	6
CTAGAAC	240	0.0	19.270832	3
GTTCTAG	260	0.0	19.211538	1
CTTATAC	720	0.0	18.5	37
TGATTCG	80	1.6164382E-5	18.5	13
CGCGCTT	50	0.0070344596	18.499998	12
TTGTATA	50	0.0070344596	18.499998	2
GGTATAC	50	0.0070344596	18.499998	3
GTCTAGA	235	0.0	18.106384	1
TAGGACA	125	8.574716E-9	17.76	4
GTCAAAA	200	0.0	17.574999	31
TTGCGTT	75	2.0670361E-4	17.266666	4
TAGAACT	280	0.0	17.178572	4
>>END_MODULE