FastQCFastQC Report
Fri 10 Feb 2017
ERR1631552.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631552.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37768
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA720.19063757678457952No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC640.16945562380851514No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC610.161512391442491No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC540.14297818258843464No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG450.11914848549036221No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC450.11914848549036221No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC380.10061427663630587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA450.003758703120.5555552
GGTATCA500.00691266118.51
CTCTTAT1257.836206E-917.7637
TCTCTTA1703.420064E-713.05882436
GTCTCTT2155.8058886E-610.32558235
GCTGTCT1500.00317715189.86666737
TGTCTCT2801.3617773E-69.2537
CTGTCTC3655.735381E-67.6027437
Produced by FastQC (version 0.11.5)