##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631552.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37768
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52674221563228	34.0	31.0	34.0	31.0	34.0
2	32.71417602202923	34.0	31.0	34.0	31.0	34.0
3	32.807164795594154	34.0	31.0	34.0	31.0	34.0
4	36.28693603050201	37.0	37.0	37.0	35.0	37.0
5	36.09140012709172	37.0	35.0	37.0	35.0	37.0
6	36.11922791781402	37.0	36.0	37.0	35.0	37.0
7	36.11676551578056	37.0	35.0	37.0	35.0	37.0
8	36.09733107392501	37.0	35.0	37.0	35.0	37.0
9	37.833854056343995	39.0	38.0	39.0	35.0	39.0
10	37.70549142130904	39.0	37.0	39.0	35.0	39.0
11	37.767422156322816	39.0	37.0	39.0	35.0	39.0
12	37.701016733742854	39.0	37.0	39.0	35.0	39.0
13	37.745392925227705	39.0	37.0	39.0	35.0	39.0
14	39.11875132387206	40.0	38.0	41.0	36.0	41.0
15	39.0519487396738	40.0	38.0	41.0	36.0	41.0
16	39.03447362846855	40.0	38.0	41.0	36.0	41.0
17	39.00643401821648	40.0	38.0	41.0	36.0	41.0
18	38.984643084092355	40.0	38.0	41.0	36.0	41.0
19	38.9678299089176	40.0	38.0	41.0	36.0	41.0
20	38.9405316670197	40.0	38.0	41.0	36.0	41.0
21	38.91929675916119	40.0	38.0	41.0	35.0	41.0
22	38.827817199745816	40.0	38.0	41.0	35.0	41.0
23	38.76315928828638	40.0	38.0	41.0	35.0	41.0
24	38.70109616606651	40.0	38.0	41.0	35.0	41.0
25	38.70874814657911	40.0	38.0	41.0	35.0	41.0
26	38.6253971616183	40.0	38.0	41.0	35.0	41.0
27	38.542125608981145	40.0	38.0	41.0	35.0	41.0
28	38.45612687989833	40.0	38.0	41.0	34.0	41.0
29	38.37020758313916	40.0	38.0	41.0	34.0	41.0
30	38.29781825884346	40.0	38.0	41.0	34.0	41.0
31	38.25357445456471	40.0	38.0	41.0	34.0	41.0
32	38.17157381910612	40.0	38.0	41.0	34.0	41.0
33	38.07681105697945	40.0	38.0	41.0	34.0	41.0
34	38.01440372802372	40.0	37.0	41.0	33.0	41.0
35	37.96393772505825	40.0	37.0	41.0	33.0	41.0
36	37.86597119254395	40.0	37.0	41.0	33.0	41.0
37	37.80719127303537	40.0	37.0	41.0	33.0	41.0
38	37.737979241686084	40.0	37.0	41.0	33.0	41.0
39	37.6722092776954	40.0	37.0	41.0	33.0	41.0
40	37.56582291887312	39.0	36.0	41.0	33.0	41.0
41	37.45027536538869	39.0	36.0	41.0	33.0	41.0
42	37.34388900656641	39.0	36.0	41.0	33.0	41.0
43	36.43997564075408	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	2.0
20	4.0
21	4.0
22	11.0
23	28.0
24	31.0
25	61.0
26	87.0
27	107.0
28	177.0
29	214.0
30	311.0
31	428.0
32	592.0
33	801.0
34	1195.0
35	1856.0
36	3179.0
37	6047.0
38	11763.0
39	10868.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.371849184494806	18.240309256513452	12.306714679093412	27.081126879898328
2	20.429993645414108	19.4741580173692	33.0597331073925	27.036115229824194
3	20.350561321753865	20.909235331497563	27.854268163524676	30.885935183223896
4	15.200699004448209	14.922685871637364	32.879686507095954	36.99692861681847
5	17.10177928404999	33.74020334674857	32.04035162041941	17.117665748782038
6	35.900762550307135	33.433065028595635	14.694979877144673	15.971192543952553
7	31.317517475111206	28.619466214785007	19.8024782884982	20.260538021605594
8	28.595636517686927	31.193073501376826	19.143190002118196	21.068099978818047
9	27.95753018428299	13.728553272611736	18.67453929252277	39.639377250582505
10	19.524465155687352	23.72378733319212	29.564710866341876	27.18703664477865
11	38.74708748146579	20.541198898538447	19.59860199110358	21.113111628892185
12	24.226858716373652	23.75291251853421	26.244439737343782	25.77578902774836
13	31.714679093412414	17.387735649226858	23.522558779919507	27.37502647744122
14	24.44662147850032	19.238508790510487	22.307244227917813	34.00762550307138
15	27.367083245075197	25.603685659817838	19.807773776742216	27.221457318364756
16	27.952234696038975	23.787333192120315	21.4467273882652	26.813704723575515
17	26.57540775259479	24.59224740521076	22.11660665113323	26.715738191061217
18	26.853420885405633	22.090129209913155	23.803219656852363	27.253230247828853
19	27.37502647744122	23.28161406481678	22.765303961025207	26.578055496716797
20	27.229400550730777	21.981571700910823	23.043317093836052	27.745710654522348
21	29.186083456894725	22.646155475534844	22.373437830968015	25.794323236602413
22	28.65918237661512	22.209277695403514	22.45022241050625	26.68131751747511
23	27.253230247828853	21.933912306714678	23.28161406481678	27.531243380639697
24	26.480088964202498	23.20747722940055	22.998305443761915	27.31412836263504
25	27.14732048294853	22.259584833721668	22.5984960813387	27.994598601991104
26	26.816352467697524	23.604638847701757	23.17835204405846	26.40065664054226
27	27.806608769328534	21.936560050836686	22.918873120101672	27.337958059733108
28	26.474793475958485	22.579961872484645	23.7502647744122	27.19497987714467
29	26.048506672315185	22.818258843465365	24.054755348443127	27.078479135776316
30	25.063545858928194	23.975323024782885	24.13153992798136	26.82959118830756
31	27.18968438890066	22.940055073077737	22.405210760432112	27.465049777589496
32	25.916119466214788	22.43698368989621	23.41135352679517	28.235543317093835
33	25.635458589281935	22.38138106333404	23.930311374708747	28.05284897267528
34	27.134081762338486	21.552637153145522	24.004448210124973	27.30883287439102
35	26.506566405422582	21.46526159711925	25.38657064181318	26.64160135564499
36	25.113852997246344	21.957742003812754	24.8676127938996	28.060792205041306
37	25.8552213514086	21.04956576996399	25.616924380427875	27.478288498199532
38	24.544588011014614	20.38498199533997	26.8084092353315	28.262020758313916
39	25.206524041516627	20.202287650921416	27.242639271340817	27.348549036221137
40	24.7034526583351	20.689472569370896	27.478288498199532	27.128786274094473
41	23.28690955306079	20.962190213937724	28.571806820588858	27.179093412412627
42	23.79262868036433	21.13164583774624	28.675068841347173	26.40065664054226
43	21.854479983054436	20.60739250158865	29.52234696038975	28.01578055496717
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	2.5
4	4.0
5	4.0
6	7.0
7	10.0
8	8.0
9	6.0
10	11.5
11	17.0
12	17.0
13	23.5
14	30.0
15	51.5
16	73.0
17	68.5
18	64.0
19	64.0
20	71.0
21	78.0
22	80.5
23	83.0
24	113.5
25	144.0
26	144.0
27	170.5
28	197.0
29	244.5
30	292.0
31	378.0
32	464.0
33	464.0
34	604.0
35	744.0
36	907.0
37	1070.0
38	1266.5
39	1463.0
40	1463.0
41	1634.5
42	1806.0
43	1988.5
44	2171.0
45	2299.0
46	2427.0
47	2427.0
48	2607.5
49	2788.0
50	2877.0
51	2966.0
52	3089.0
53	3212.0
54	3212.0
55	3134.5
56	3057.0
57	2996.5
58	2936.0
59	2774.5
60	2613.0
61	2613.0
62	2500.5
63	2388.0
64	2183.5
65	1979.0
66	1795.5
67	1612.0
68	1612.0
69	1359.5
70	1107.0
71	965.5
72	824.0
73	604.0
74	384.0
75	384.0
76	313.5
77	243.0
78	210.5
79	178.0
80	158.5
81	139.0
82	139.0
83	103.5
84	68.0
85	65.5
86	63.0
87	53.0
88	43.0
89	43.0
90	28.0
91	13.0
92	10.0
93	7.0
94	4.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	37768.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.29209913153993
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.75796487818421	69.2411565346325
2	4.834455473033942	7.376615123914425
3	1.4263899493301866	3.2646685024359243
4	0.8329284375650725	2.541834357127727
5	0.49975706253904356	1.9063757678457953
6	0.2915249531477754	1.3344630374920567
7	0.21170264454778925	1.130586740097437
8	0.1457624765738877	0.8896420249947045
9	0.15964461719997222	1.0961660665113324
>10	0.8259873672520303	10.553908070324084
>50	0.013882140626084543	0.6645837746240203
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	72	0.19063757678457952	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	64	0.16945562380851514	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	61	0.161512391442491	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	54	0.14297818258843464	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	45	0.11914848549036221	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	45	0.11914848549036221	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	38	0.10061427663630587	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.002647744122008049	0.0
16	0.0	0.0	0.0	0.005295488244016098	0.0
17	0.0	0.0	0.0	0.010590976488032196	0.0
18	0.0	0.0	0.0	0.010590976488032196	0.0
19	0.0	0.0	0.0	0.010590976488032196	0.0
20	0.0	0.0	0.0	0.013238720610040246	0.0
21	0.0	0.0	0.0	0.018534208854056343	0.0
22	0.0	0.0	0.0	0.021181952976064393	0.0
23	0.0	0.0	0.0	0.021181952976064393	0.0
24	0.0	0.0	0.0	0.037068417708112686	0.0
25	0.0	0.0	0.0	0.042363905952128786	0.0
26	0.0	0.0	0.0	0.06354585892819319	0.0
27	0.0	0.0	0.0	0.09531878839228977	0.0
28	0.0	0.0	0.0	0.2912518534208854	0.0
29	0.0	0.0	0.0	0.5083668714255455	0.0
30	0.0	0.0	0.0	0.7969709807244227	0.0
31	0.0	0.0	0.0	1.5992374496928616	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	45	0.0037587031	20.555555	2
GGTATCA	50	0.006912661	18.5	1
CTCTTAT	125	7.836206E-9	17.76	37
TCTCTTA	170	3.420064E-7	13.058824	36
GTCTCTT	215	5.8058886E-6	10.325582	35
GCTGTCT	150	0.0031771518	9.866667	37
TGTCTCT	280	1.3617773E-6	9.25	37
CTGTCTC	365	5.735381E-6	7.60274	37
>>END_MODULE