FastQCFastQC Report
Fri 10 Feb 2017
ERR1631551.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631551.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285800
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT194250.8498118820544229No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT140680.6154519205529793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT138430.605608539679762No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57220.25032811269577393No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA51250.22421034211217078No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC33820.14795695161431446No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA27660.12100796220141745No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA25900.11330825094058973No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC25050.10958964038848543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA802.7288479E-823.1252
TTCGGTA400.001931768723.12526
TAACCGG759.272348E-619.73333422
CAGTCGG29150.019.5471710
AGTCGGT29000.019.45689811
GCAGTCG29200.019.3236319
GGCAGTC29250.019.227358
GGGCAGT30550.018.7119487
GTTATAC1306.9849193E-1018.5000023
TCGGTGA31000.018.02258113
CGGTGAT31350.017.88038314
GACAGGC9500.017.7210527
TAGCGAA953.6095444E-617.52631610
GTCGGTG31950.017.42879712
GCCTTAT22450.017.30512226
GTGATTC31500.017.20793716
CTTATAC49700.017.08551237
CGCTTAT8150.017.0245426
CGCCTTA22900.016.96506525
TAAGGTA1201.0424992E-716.9583345
Produced by FastQC (version 0.11.5)