##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631551.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2285800
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06106046023274	34.0	33.0	34.0	31.0	34.0
2	33.190112870767344	34.0	33.0	34.0	31.0	34.0
3	33.26031630063873	34.0	33.0	34.0	31.0	34.0
4	36.49772814769446	37.0	37.0	37.0	35.0	37.0
5	36.50585440545979	37.0	37.0	37.0	35.0	37.0
6	36.57018461807682	37.0	37.0	37.0	35.0	37.0
7	36.56787732960014	37.0	37.0	37.0	35.0	37.0
8	36.57380741972176	37.0	37.0	37.0	35.0	37.0
9	38.41955376673375	39.0	39.0	39.0	37.0	39.0
10	38.38529617639338	39.0	39.0	39.0	37.0	39.0
11	38.442320412984515	39.0	39.0	39.0	37.0	39.0
12	38.40572447283227	39.0	39.0	39.0	37.0	39.0
13	38.428351124332835	39.0	39.0	39.0	37.0	39.0
14	39.9528493306501	41.0	40.0	41.0	38.0	41.0
15	39.946055210429606	41.0	40.0	41.0	38.0	41.0
16	39.92128401434946	41.0	40.0	41.0	38.0	41.0
17	39.9164979438271	41.0	40.0	41.0	38.0	41.0
18	39.917576340887216	41.0	40.0	41.0	38.0	41.0
19	39.94810875842156	41.0	40.0	41.0	38.0	41.0
20	39.93830475107183	41.0	40.0	41.0	38.0	41.0
21	39.908251378073324	41.0	40.0	41.0	38.0	41.0
22	39.85384591827806	41.0	40.0	41.0	38.0	41.0
23	39.78924971563566	41.0	40.0	41.0	38.0	41.0
24	39.76365342549654	41.0	40.0	41.0	37.0	41.0
25	39.73652244290839	41.0	40.0	41.0	37.0	41.0
26	39.63211479569516	41.0	40.0	41.0	37.0	41.0
27	39.567858955289175	41.0	40.0	41.0	36.0	41.0
28	39.47543223379123	41.0	39.0	41.0	36.0	41.0
29	39.39503806107271	41.0	39.0	41.0	36.0	41.0
30	39.2896272639776	41.0	39.0	41.0	35.0	41.0
31	39.191100708723425	41.0	39.0	41.0	35.0	41.0
32	39.08208285939277	41.0	39.0	41.0	35.0	41.0
33	38.97377898328813	41.0	39.0	41.0	35.0	41.0
34	38.88978957039111	41.0	39.0	41.0	35.0	41.0
35	38.758823606614754	41.0	38.0	41.0	35.0	41.0
36	38.666374573453496	40.0	38.0	41.0	35.0	41.0
37	38.56448551929303	40.0	38.0	41.0	35.0	41.0
38	38.45169393647738	40.0	38.0	41.0	35.0	41.0
39	38.321368448683174	40.0	38.0	41.0	35.0	41.0
40	38.17152288039198	40.0	38.0	41.0	35.0	41.0
41	38.01959007787208	40.0	37.0	41.0	35.0	41.0
42	37.83927858955289	40.0	37.0	41.0	34.0	41.0
43	37.02246522005425	40.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	6.0
11	21.0
12	12.0
13	12.0
14	6.0
15	9.0
16	22.0
17	17.0
18	35.0
19	66.0
20	131.0
21	257.0
22	468.0
23	703.0
24	1326.0
25	2051.0
26	3342.0
27	4940.0
28	7543.0
29	10731.0
30	14276.0
31	17575.0
32	21221.0
33	27306.0
34	40933.0
35	62046.0
36	96441.0
37	215800.0
38	426547.0
39	1331953.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.807288476682125	17.0855280426984	13.268352436783621	25.838831043835857
2	18.730072622276666	20.02406159769009	35.03897978825794	26.20688599177531
3	19.654344212092045	22.86105521042961	28.984731822556654	28.499868754921692
4	13.848411934552454	15.875492169043662	33.71511068334937	36.56098521305451
5	15.207192230291364	36.529180155744164	31.932846268264942	16.330781345699535
6	33.302082421909176	33.79167031236329	17.140432233791234	15.765815031936302
7	30.16108145944527	30.02716773121008	20.45760783970601	19.354142969638637
8	29.839618514305716	31.22569778633301	19.092571528567678	19.842112170793598
9	28.448464432583776	13.76747746959489	18.98871292326538	38.795345174555955
10	19.057529092659024	25.573715985650537	29.172368536179892	26.196386385510547
11	36.20154869192405	22.52992387785458	19.72119170531105	21.547335724910315
12	23.852305538542304	25.99068159944002	26.928996412634525	23.228016449383148
13	30.928121445445793	18.469638638551054	25.19135532417534	25.41088459182781
14	23.60678974538455	20.11155831656313	24.90970338612302	31.3719485519293
15	27.113876979613266	25.896010149619393	21.8732610027124	25.116851868054944
16	25.280295738909793	25.060941464695073	24.095765158806547	25.56299763758859
17	23.43131507568466	26.433896228891417	25.096115145682035	25.038673549741887
18	24.572009799632514	23.119914253215505	27.28532680024499	25.022749146906992
19	24.842462157669086	24.94378335812407	26.7223291626564	23.49142532155044
20	25.486919240528483	23.251815556916615	26.91272202292414	24.348543179630763
21	27.753215504418584	23.09847755709161	25.957345349549392	23.190961588940414
22	26.621051710560856	23.206448508180944	25.174643450870594	24.99785633038761
23	24.493350249365648	23.933896228891417	25.980794470207368	25.591959051535568
24	24.332312538279815	24.490156619126783	26.71795432671275	24.459576515880656
25	24.268177443345877	23.65766908740922	26.32181293201505	25.752340537229855
26	25.263977600839972	24.412940764721323	26.42287164231341	23.900209992125294
27	25.60486481756934	23.62437658587803	25.41031586315513	25.360442733397498
28	23.808032198792546	23.879692011549565	27.463863855105437	24.848411934552452
29	23.73051010587103	24.08929040160994	27.820237991075338	24.359961501443696
30	23.131770058622802	24.664712573278504	28.150100621226702	24.053416746871992
31	24.07970951089334	24.795607664712573	26.349811882054425	24.774870942339664
32	23.339006037273602	24.105083559366523	27.438489806632248	25.117420596727623
33	23.478563303876104	23.77338349811882	27.75728410184618	24.990769096158893
34	24.807025986525506	23.787907953451747	26.65793157756584	24.747134482456907
35	24.317481844430834	24.02414909440896	27.28475807157232	24.373610989587892
36	22.708548429433897	24.581809432146294	27.15591915303176	25.55372298538805
37	23.36682999387523	23.541079709510893	27.73567241228454	25.356417884329336
38	22.925365298801296	22.551491819056785	29.04401084959314	25.47913203254878
39	23.10775220929215	22.7708460932715	28.896403884854315	25.22499781258203
40	22.581415696911368	22.750240615976903	29.11475194680199	25.553591740309738
41	20.8367311225829	23.48779420771721	30.24109720885467	25.43437746084522
42	21.67582465657538	23.68321812932015	29.300594977688338	25.340362236416137
43	20.542479657012862	23.32833143757109	29.985956776620874	26.14323212879517
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	137.0
1	235.5
2	334.0
3	1492.0
4	2650.0
5	2650.0
6	3631.0
7	4612.0
8	4418.0
9	4224.0
10	6287.0
11	8350.0
12	8350.0
13	13397.0
14	18444.0
15	28001.5
16	37559.0
17	33026.0
18	28493.0
19	28493.0
20	29976.0
21	31459.0
22	24035.0
23	16611.0
24	17945.0
25	19279.0
26	19279.0
27	21333.5
28	23388.0
29	26992.5
30	30597.0
31	35760.5
32	40924.0
33	40924.0
34	49017.0
35	57110.0
36	67676.5
37	78243.0
38	88209.0
39	98175.0
40	98175.0
41	108119.0
42	118063.0
43	127057.0
44	136051.0
45	145896.5
46	155742.0
47	155742.0
48	167458.5
49	179175.0
50	177877.0
51	176579.0
52	169795.0
53	163011.0
54	163011.0
55	156148.5
56	149286.0
57	141858.5
58	134431.0
59	128473.0
60	122515.0
61	122515.0
62	115958.0
63	109401.0
64	100632.0
65	91863.0
66	82291.5
67	72720.0
68	72720.0
69	62718.5
70	52717.0
71	46801.0
72	40885.0
73	35672.5
74	30460.0
75	30460.0
76	27015.0
77	23570.0
78	18433.0
79	13296.0
80	9847.5
81	6399.0
82	6399.0
83	4917.5
84	3436.0
85	3065.0
86	2694.0
87	2315.0
88	1936.0
89	1936.0
90	1379.0
91	822.0
92	464.0
93	106.0
94	71.5
95	37.0
96	37.0
97	23.5
98	10.0
99	8.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2285800.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.92928488055086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.3893137004402	38.36480801001802
2	7.740599889061314	6.955592351239923
3	2.295088837080674	3.0935010056210936
4	1.083404726386152	1.9470639837095471
5	0.6342011032458912	1.4247101019647146
6	0.4280826433981448	1.1540068222592705
7	0.32453027638883125	1.0206639268166398
8	0.23015273646192683	0.8272478290029004
9	0.19252303840272786	0.7784930194618853
>10	1.311088525635991	11.979808679655255
>50	0.1913507769122003	5.971766128380417
>100	0.15572995484983407	14.220393225006504
>500	0.01553254239201009	4.65481025801731
>1k	0.007912804614797592	5.053427818536605
>5k	1.9537789172339732E-4	0.4760850780613062
>10k+	2.9306683758509604E-4	2.077621762248547
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19425	0.8498118820544229	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14068	0.6154519205529793	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13843	0.605608539679762	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5722	0.25032811269577393	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	5125	0.22421034211217078	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	3382	0.14795695161431446	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2766	0.12100796220141745	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	2590	0.11330825094058973	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2505	0.10958964038848543	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.749671887304226E-5	0.0
2	4.374835943652113E-5	0.0	0.0	8.749671887304226E-5	0.0
3	4.374835943652113E-5	0.0	0.0	8.749671887304226E-5	0.0
4	4.374835943652113E-5	0.0	0.0	8.749671887304226E-5	0.0
5	4.374835943652113E-5	0.0	0.0	1.312450783095634E-4	0.0
6	4.374835943652113E-5	0.0	0.0	1.312450783095634E-4	0.0
7	4.374835943652113E-5	0.0	0.0	1.312450783095634E-4	0.0
8	4.374835943652113E-5	0.0	0.0	1.312450783095634E-4	0.0
9	4.374835943652113E-5	0.0	0.0	1.7499343774608452E-4	4.374835943652113E-5
10	4.374835943652113E-5	0.0	0.0	6.562253915478169E-4	4.374835943652113E-5
11	4.374835943652113E-5	0.0	0.0	0.0018374310963338875	4.374835943652113E-5
12	4.374835943652113E-5	0.0	0.0	0.0031936302388660427	4.374835943652113E-5
13	4.374835943652113E-5	0.0	0.0	0.003674862192667775	4.374835943652113E-5
14	4.374835943652113E-5	0.0	0.0	0.0052935514918190565	4.374835943652113E-5
15	4.374835943652113E-5	0.0	0.0	0.0077872079797007615	4.374835943652113E-5
16	4.374835943652113E-5	0.0	0.0	0.012643275877154607	4.374835943652113E-5
17	8.749671887304226E-5	0.0	0.0	0.017236853617989326	4.374835943652113E-5
18	8.749671887304226E-5	0.0	0.0	0.019293026511505818	4.374835943652113E-5
19	8.749671887304226E-5	0.0	0.0	0.021392947764458832	4.374835943652113E-5
20	8.749671887304226E-5	0.0	0.0	0.02445533292501531	4.374835943652113E-5
21	8.749671887304226E-5	0.0	0.0	0.030405109808382187	1.312450783095634E-4
22	8.749671887304226E-5	0.0	0.0	0.04042348411934552	1.312450783095634E-4
23	8.749671887304226E-5	0.0	0.0	0.05678537054860443	1.312450783095634E-4
24	8.749671887304226E-5	0.0	0.0	0.08080321987925453	1.312450783095634E-4
25	8.749671887304226E-5	0.0	0.0	0.08994662700148745	2.1874179718260565E-4
26	8.749671887304226E-5	0.0	4.374835943652113E-5	0.1037273602239916	2.1874179718260565E-4
27	8.749671887304226E-5	0.0	4.374835943652113E-5	0.14835068684924316	2.1874179718260565E-4
28	1.312450783095634E-4	0.0	4.374835943652113E-5	0.3244815819406772	2.1874179718260565E-4
29	1.312450783095634E-4	0.0	4.374835943652113E-5	0.5950651850555604	2.1874179718260565E-4
30	1.312450783095634E-4	0.0	4.374835943652113E-5	1.0487794207717211	2.1874179718260565E-4
31	1.312450783095634E-4	0.0	4.374835943652113E-5	1.9164843818356812	2.1874179718260565E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATA	80	2.7288479E-8	23.125	2
TTCGGTA	40	0.0019317687	23.125	26
TAACCGG	75	9.272348E-6	19.733334	22
CAGTCGG	2915	0.0	19.54717	10
AGTCGGT	2900	0.0	19.456898	11
GCAGTCG	2920	0.0	19.323631	9
GGCAGTC	2925	0.0	19.22735	8
GGGCAGT	3055	0.0	18.711948	7
GTTATAC	130	6.9849193E-10	18.500002	3
TCGGTGA	3100	0.0	18.022581	13
CGGTGAT	3135	0.0	17.880383	14
GACAGGC	950	0.0	17.721052	7
TAGCGAA	95	3.6095444E-6	17.526316	10
GTCGGTG	3195	0.0	17.428797	12
GCCTTAT	2245	0.0	17.305122	26
GTGATTC	3150	0.0	17.207937	16
CTTATAC	4970	0.0	17.085512	37
CGCTTAT	815	0.0	17.02454	26
CGCCTTA	2290	0.0	16.965065	25
TAAGGTA	120	1.0424992E-7	16.958334	5
>>END_MODULE