FastQCFastQC Report
Fri 10 Feb 2017
ERR1631550.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631550.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45461
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15463.4007170981720596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15223.347924594707551No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10862.388860781768989No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6091.3396097754118916No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.5169265964233078No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2270.49932909526847186No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.3871450254063923No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.29915751963221227No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1320.29035876905479424No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1190.2617628296781857No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.2551637667451222No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.22216845207980468No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA990.21776907679109564No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.21336970150238666No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA910.20017157563625965No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA850.18697344977013264No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.18697344977013264No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA840.18477376212577815No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.18477376212577815No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC810.17817469919271464No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.17377532390400563No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG740.16277688568223314No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT730.16057719803787862No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC710.15617782274916964No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT710.15617782274916964No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA700.15397813510481512No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.15177844746046062No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT680.14957875981610613No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT660.14517938452739712No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG660.14517938452739712No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA640.1407800092386881No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC620.1363806339499791No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT590.1297815710169156No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT550.1209828204394976No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC550.1209828204394976No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.1209828204394976No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT490.10778469457337059No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA490.10778469457337059No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG480.10558500692901607No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT470.10338531928466158No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT470.10338531928466158No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT470.10338531928466158No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG470.10338531928466158No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC460.10118563164030708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG200.001823400537.05
GGACCGT200.001823400537.06
GACCGTT200.001823400537.07
ATGCCCC358.738468E-426.4285737
TGGGCAG1950.021.8205136
TATACAC603.6259866E-521.58333237
GGGCAGT2000.021.2750027
AGTCGGT2050.020.75609611
AGAGCTG450.00377039620.55555534
TCCTGTC450.00377039620.55555533
GGCAGTC2100.020.2619048
TTATACA656.71765E-519.92307736
CAGTCGG2150.019.79069710
GCAGTCG2150.019.7906979
CGCTTAT609.0521306E-418.526
GAGCTGT500.006933962518.535
CTCCGCT609.0521306E-418.523
GCTTATT801.5617601E-518.527
GGTATCA6900.018.2318841
TCGGTGA2350.018.10638213
Produced by FastQC (version 0.11.5)