FastQCFastQC Report
Fri 10 Feb 2017
ERR1631549.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631549.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89160
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1220.13683266038582323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.1155226558995065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT980.10991475998205474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT950.10655002243158368No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC900.1009421265141319No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAG250.005469525729.61
CGAGAGG250.005469525729.625
GTCTTAG250.005469525729.61
ATACACA502.673478E-729.637
GGTATCA1150.027.3478261
AAGCAGG358.805115E-426.428576
GCATGCA358.805115E-426.428579
CCATGAG400.001917323123.1250023
TTAAGCC400.001917323123.1250023
CCGAGAG400.001917323123.12500224
TATACAC1151.2914825E-1020.91304437
GTATCAA1650.020.1818162
CCCCTTG500.006985340318.57
CGGGTTA700.002567431415.8571427
CCTCGAT700.002567431415.85714227
ATCAACG1953.8198777E-1115.1794874
AACGCAG1953.8198777E-1115.1794877
CAACGCA2005.820766E-1114.86
CTCGATG750.00406505314.828
TCAACGG750.00406505314.83
Produced by FastQC (version 0.11.5)