FastQCFastQC Report
Fri 10 Feb 2017
ERR1631548.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631548.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235056
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31301.3315975767476687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27941.1886529167517528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22100.9402014839017085No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9410.4003301340957049No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA9070.38586549588183244No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA5360.228030767136342No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA4140.17612824178068204No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA4070.17315022803076713No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.15655843713838405No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT3430.1459226737458308No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT3420.14549724321012866No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC3320.14124293785310735No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA3250.13826492410319244No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT3200.13613777142468178No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC3190.13571234088897963No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.1348614798175754No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT2980.12677829963923493No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2820.11997141106800081No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA2790.11869511946089444No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA2690.11444081410387312No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2610.11103736981825608No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2540.10805935606834115No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA2530.10763392553263904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC250.005487562629.5999983
TAACGAT250.005487562629.59999823
GGAAAAT250.005487562629.59999819
CGTAACG250.005487562629.59999821
TATCGGG250.005487562629.5999982
AAAGCCG250.005487562629.59999815
CAAAGCA453.990446E-628.7777794
GTTCAAA405.9203507E-527.7500021
GCCCGAG405.9203507E-527.75000212
TATTAGC509.050978E-625.8999982
ATTAGCA451.3188417E-424.6666683
TTAGCAA451.3188417E-424.6666684
AACTCAT451.3188417E-424.66666810
TATGCTG400.001926644523.12500236
AAATTTC400.001926644523.12500227
CTGGATA400.001926644523.12500223
AAACAGG502.6932507E-422.19999923
CGGTTGG603.7100785E-521.58333233
ATCTTAC705.073487E-621.1428571
ATACCAT705.073487E-621.1428576
Produced by FastQC (version 0.11.5)