##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631548.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	235056
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97045384929549	34.0	33.0	34.0	31.0	34.0
2	33.105430195357705	34.0	33.0	34.0	31.0	34.0
3	33.14765417602614	34.0	33.0	34.0	31.0	34.0
4	36.40578415356341	37.0	37.0	37.0	35.0	37.0
5	36.42771509767885	37.0	37.0	37.0	35.0	37.0
6	36.51285651078892	37.0	37.0	37.0	35.0	37.0
7	36.51925498604588	37.0	37.0	37.0	35.0	37.0
8	36.533072969845485	37.0	37.0	37.0	35.0	37.0
9	38.36442379688245	39.0	39.0	39.0	37.0	39.0
10	38.330457422911984	39.0	39.0	39.0	37.0	39.0
11	38.38732897692465	39.0	39.0	39.0	37.0	39.0
12	38.362781635014635	39.0	39.0	39.0	37.0	39.0
13	38.384891259955076	39.0	39.0	39.0	37.0	39.0
14	39.85612790143625	41.0	40.0	41.0	38.0	41.0
15	39.82904924783881	41.0	40.0	41.0	38.0	41.0
16	39.75008083180178	41.0	40.0	41.0	38.0	41.0
17	39.77073973861548	41.0	40.0	41.0	38.0	41.0
18	39.836749540535024	41.0	40.0	41.0	38.0	41.0
19	39.89109829147097	41.0	40.0	41.0	38.0	41.0
20	39.87302600231434	41.0	40.0	41.0	38.0	41.0
21	39.858629432986184	41.0	40.0	41.0	38.0	41.0
22	39.797435504730785	41.0	40.0	41.0	38.0	41.0
23	39.73752637669321	41.0	40.0	41.0	37.0	41.0
24	39.70850350554761	41.0	40.0	41.0	37.0	41.0
25	39.69619154584439	41.0	40.0	41.0	37.0	41.0
26	39.576394561296034	41.0	40.0	41.0	37.0	41.0
27	39.47743516438636	41.0	39.0	41.0	36.0	41.0
28	39.383487339187255	41.0	39.0	41.0	36.0	41.0
29	39.28480021782043	41.0	39.0	41.0	35.0	41.0
30	39.13957950445851	41.0	39.0	41.0	35.0	41.0
31	38.99035123545028	40.0	39.0	41.0	35.0	41.0
32	38.86680195357702	40.0	38.0	41.0	35.0	41.0
33	38.73572255122183	40.0	38.0	41.0	35.0	41.0
34	38.666968722347015	40.0	38.0	41.0	35.0	41.0
35	38.5370507453543	40.0	38.0	41.0	35.0	41.0
36	38.42691954257709	40.0	38.0	41.0	35.0	41.0
37	38.30376591110203	40.0	38.0	41.0	35.0	41.0
38	38.17289071540399	40.0	37.0	41.0	35.0	41.0
39	38.00678561704445	40.0	37.0	41.0	35.0	41.0
40	37.83309083792798	40.0	37.0	41.0	34.0	41.0
41	37.61814205976448	40.0	37.0	41.0	34.0	41.0
42	37.38595058198897	40.0	36.0	41.0	33.0	41.0
43	36.57821114968348	40.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	0.0
15	0.0
16	2.0
17	1.0
18	2.0
19	5.0
20	11.0
21	19.0
22	51.0
23	91.0
24	165.0
25	240.0
26	377.0
27	576.0
28	908.0
29	1318.0
30	1718.0
31	2051.0
32	2503.0
33	3202.0
34	4925.0
35	6890.0
36	10765.0
37	26245.0
38	46766.0
39	126223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.95572118984412	17.792355864134503	14.718620243686612	24.533302702334765
2	19.362194540875365	20.539786263698865	35.05632700292696	25.04169219249881
3	19.260516642842557	24.310802532162548	29.97668640664352	26.451994418351372
4	13.187921176230343	18.329249200190592	34.81723504186237	33.6655945817167
5	13.36490027908243	38.44828466408005	33.1078551494112	15.078959907426315
6	28.957354843101218	34.895088829895855	18.959311823565447	17.188244503437478
7	28.845466612211556	31.515894084813834	20.766115308692395	18.872523994282215
8	30.465506092165274	30.801596215369955	18.683632836430466	20.049264856034306
9	28.571063916683688	13.887328976924648	19.7480600367572	37.79354706963447
10	20.41088081138112	26.427319447280652	27.808266966169764	25.353532775168468
11	33.06148322101967	25.633891498196178	18.499421414471446	22.80520386631271
12	23.103430671839902	29.624004492546458	25.559866584984004	21.712698250629636
13	31.250850861071406	19.835273296576137	25.61304540194677	23.30083044040569
14	23.034510925056157	22.029643999727725	25.932118303723367	29.00372677149275
15	29.336838880947518	25.83426928051188	22.069634470083724	22.75925736845688
16	23.943230549315906	25.91084677693826	26.334150159961883	23.81177251378395
17	21.021799060649375	29.176026138452116	26.05081342318426	23.751361377714247
18	22.1245150091893	24.011724865563952	29.022445715063643	24.841314410183106
19	22.679276427744878	27.56024096385542	29.289190660948883	20.47129194745082
20	23.637345994146074	23.89175345449595	30.653546388945614	21.81735416241236
21	26.026989313184945	23.722857531822203	28.809305016676877	21.440848138315978
22	25.898934721938605	23.408889796474032	26.76340957048533	23.928765911102033
23	23.173626710230756	25.1391157851746	27.79167517527738	23.895582329317268
24	21.781617997413385	26.37201347763937	28.657851745966916	23.18851677898033
25	22.17471581240215	25.108910217139744	28.454921380437003	24.2614525900211
26	23.96024777074399	24.917041045538085	27.944404737594446	23.17830644612348
27	23.008559662378328	24.972772445715062	27.10290313797563	24.915764753930976
28	21.72078143080798	25.35565992784698	29.98732217003608	22.936236471308966
29	22.035174596691853	25.499030018378598	30.643761486624467	21.822033898305087
30	20.989466339936016	26.58430331495473	31.05217480089851	21.37405554421074
31	22.11430467633245	26.941239534408822	28.3945102443673	22.549945544891433
32	20.641889592267372	26.241406303178817	30.833928936083314	22.282775168470494
33	20.67677489619495	25.43734259070179	31.222772445715062	22.6631100673882
34	22.762235382206793	25.399053842488602	28.457048533115515	23.381662242189094
35	21.68334354366619	26.802123749234223	28.77144169899939	22.743091008100198
36	20.001191205499964	26.808505207269757	29.016064257028113	24.174239330202166
37	20.81844326458376	25.6589918998026	29.920955006466542	23.601609829147097
38	20.4274726022735	23.812197944319653	31.31594173303383	24.44438772037302
39	20.7069804642298	24.144033762167314	31.796252807841537	23.35273296576135
40	20.265808998706692	23.7619971411068	31.23170648696481	24.7404873732217
41	17.94125655163025	24.78813559322034	32.832601592811926	24.438006262337485
42	18.03868014430604	24.607327615546932	32.42078483425227	24.933207405894766
43	17.422656728609354	25.368848274453747	32.97809883602205	24.230396160914847
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	23.0
2	40.0
3	136.5
4	233.0
5	233.0
6	346.5
7	460.0
8	453.0
9	446.0
10	756.5
11	1067.0
12	1067.0
13	1975.5
14	2884.0
15	4538.5
16	6193.0
17	5310.0
18	4427.0
19	4427.0
20	4846.5
21	5266.0
22	3565.5
23	1865.0
24	1764.0
25	1663.0
26	1663.0
27	1641.0
28	1619.0
29	2184.0
30	2749.0
31	3415.0
32	4081.0
33	4081.0
34	5188.5
35	6296.0
36	7386.0
37	8476.0
38	9528.0
39	10580.0
40	10580.0
41	12265.5
42	13951.0
43	15604.0
44	17257.0
45	19066.0
46	20875.0
47	20875.0
48	24062.5
49	27250.0
50	25746.5
51	24243.0
52	20494.5
53	16746.0
54	16746.0
55	14351.0
56	11956.0
57	10165.0
58	8374.0
59	8138.0
60	7902.0
61	7902.0
62	7485.5
63	7069.0
64	6478.5
65	5888.0
66	5467.0
67	5046.0
68	5046.0
69	4328.0
70	3610.0
71	3104.0
72	2598.0
73	2037.5
74	1477.0
75	1477.0
76	1213.0
77	949.0
78	770.0
79	591.0
80	488.5
81	386.0
82	386.0
83	289.5
84	193.0
85	171.5
86	150.0
87	120.5
88	91.0
89	91.0
90	78.0
91	65.0
92	43.5
93	22.0
94	16.0
95	10.0
96	10.0
97	7.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	235056.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.530954325777685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.6982309134305	32.68412633585188
2	8.386515196525338	6.9659995915866855
3	3.58939162680161	4.472125791300796
4	1.943229428094365	3.2281669049077664
5	1.3736798434763011	2.8525117418827852
6	0.9629075711168703	2.3994282213600164
7	0.6812058880773605	1.9803791436934177
8	0.530623533870786	1.762984139949629
9	0.4927218528800155	1.841688789054523
>10	2.998330277296893	23.66414811789531
>50	0.24584874156175412	7.117878292832347
>100	0.09116890832915049	6.555884555169832
>500	0.0030731092695219266	1.0142263971138794
>1k	0.0030731092695219266	3.4604519774011298
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3130	1.3315975767476687	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2794	1.1886529167517528	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2210	0.9402014839017085	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	941	0.4003301340957049	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	907	0.38586549588183244	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	536	0.228030767136342	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	414	0.17612824178068204	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	407	0.17315022803076713	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	368	0.15655843713838405	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	343	0.1459226737458308	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	342	0.14549724321012866	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	332	0.14124293785310735	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	325	0.13826492410319244	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	320	0.13613777142468178	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	319	0.13571234088897963	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	317	0.1348614798175754	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	298	0.12677829963923493	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	282	0.11997141106800081	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	279	0.11869511946089444	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	269	0.11444081410387312	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	261	0.11103736981825608	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	254	0.10805935606834115	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	253	0.10763392553263904	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.002978013749914914	0.0
13	0.0	0.0	0.0	0.004254305357021306	0.0
14	0.0	0.0	0.0	0.006381458035531958	0.0
15	0.0	0.0	0.0	0.011061193928255395	0.0
16	0.0	0.0	0.0	0.025525832142127832	0.0
17	0.0	0.0	0.0	0.042543053570213055	0.0
18	0.0	0.0	0.0	0.05062623374855354	0.0
19	0.0	0.0	0.0	0.06594173303383023	0.0
20	0.0	0.0	0.0	0.07827921856919202	0.0
21	0.0	0.0	0.0	0.09529643999727724	0.0
22	0.0	0.0	0.0	0.1306071744605541	0.0
23	0.0	0.0	0.0	0.17017221428085222	0.0
24	0.0	0.0	0.0	0.23483765570757606	0.0
25	0.0	0.0	0.0	0.25228030767136345	0.0
26	0.0	0.0	0.0	0.28333673677761895	0.0
27	0.0	0.0	0.0	0.33396297052617246	0.0
28	0.0	0.0	0.0	0.4441494792730243	0.0
29	0.0	0.0	0.0	0.6202777210537064	0.0
30	0.0	0.0	0.0	0.9542406915798788	0.0
31	0.0	0.0	0.0	1.7765979170920971	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAATAC	25	0.0054875626	29.599998	3
TAACGAT	25	0.0054875626	29.599998	23
GGAAAAT	25	0.0054875626	29.599998	19
CGTAACG	25	0.0054875626	29.599998	21
TATCGGG	25	0.0054875626	29.599998	2
AAAGCCG	25	0.0054875626	29.599998	15
CAAAGCA	45	3.990446E-6	28.777779	4
GTTCAAA	40	5.9203507E-5	27.750002	1
GCCCGAG	40	5.9203507E-5	27.750002	12
TATTAGC	50	9.050978E-6	25.899998	2
ATTAGCA	45	1.3188417E-4	24.666668	3
TTAGCAA	45	1.3188417E-4	24.666668	4
AACTCAT	45	1.3188417E-4	24.666668	10
TATGCTG	40	0.0019266445	23.125002	36
AAATTTC	40	0.0019266445	23.125002	27
CTGGATA	40	0.0019266445	23.125002	23
AAACAGG	50	2.6932507E-4	22.199999	23
CGGTTGG	60	3.7100785E-5	21.583332	33
ATCTTAC	70	5.073487E-6	21.142857	1
ATACCAT	70	5.073487E-6	21.142857	6
>>END_MODULE