FastQCFastQC Report
Fri 10 Feb 2017
ERR1631547.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631547.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243254
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.1348384815871476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3260.13401629572381132No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2510.10318432584870137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTTT451.3190272E-424.66666624
TGCAAGA451.3190272E-424.6666662
TGGAGGT400.001926837423.1254
GGTATCA1850.023.01
CGATTTG502.6936256E-422.19999928
GAACCGC502.6936256E-422.1999996
ACCTCCG603.710762E-521.58333419
TATACAC957.075869E-921.42105336
GTGCAAG450.00381727320.5555551
CGCGGTC450.00381727320.55555510
AGAGTGT450.00381727320.5555556
ATACACA1001.2772944E-820.34999837
GCCTTTC555.127091E-420.1818187
GATTTGT555.127091E-420.18181829
ATCGTCT555.127091E-420.1818188
CCGACTT555.127091E-420.18181823
TCGTTCT609.2083064E-418.530
TTCGTTC609.2083064E-418.529
ATAGAAC701.21419E-418.53
TGGCCGT701.21419E-418.56
Produced by FastQC (version 0.11.5)