##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631547.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	243254
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.012719215305815	34.0	33.0	34.0	31.0	34.0
2	33.166500859184225	34.0	33.0	34.0	31.0	34.0
3	33.25399787876047	34.0	34.0	34.0	31.0	34.0
4	36.47773520682085	37.0	37.0	37.0	35.0	37.0
5	36.46088039662246	37.0	37.0	37.0	35.0	37.0
6	36.52194002976313	37.0	37.0	37.0	35.0	37.0
7	36.53278466130053	37.0	37.0	37.0	35.0	37.0
8	36.527539115492445	37.0	37.0	37.0	35.0	37.0
9	38.380125301125574	39.0	39.0	39.0	37.0	39.0
10	38.3421320923808	39.0	39.0	39.0	37.0	39.0
11	38.39815994803785	39.0	39.0	39.0	37.0	39.0
12	38.3589129058515	39.0	39.0	39.0	37.0	39.0
13	38.386711009890895	39.0	39.0	39.0	37.0	39.0
14	39.93644914369342	41.0	40.0	41.0	38.0	41.0
15	39.92039185378247	41.0	40.0	41.0	38.0	41.0
16	39.884088236986855	41.0	40.0	41.0	38.0	41.0
17	39.876474795892356	41.0	40.0	41.0	38.0	41.0
18	39.841585338781684	41.0	40.0	41.0	38.0	41.0
19	39.86399812541623	41.0	40.0	41.0	38.0	41.0
20	39.82443454167249	41.0	40.0	41.0	38.0	41.0
21	39.79846991210833	41.0	40.0	41.0	38.0	41.0
22	39.748259021434386	41.0	40.0	41.0	38.0	41.0
23	39.695293808118265	41.0	40.0	41.0	37.0	41.0
24	39.67899397337762	41.0	40.0	41.0	37.0	41.0
25	39.66711749858173	41.0	40.0	41.0	37.0	41.0
26	39.57693604216169	41.0	40.0	41.0	37.0	41.0
27	39.52507255790244	41.0	40.0	41.0	37.0	41.0
28	39.470212206171325	41.0	39.0	41.0	37.0	41.0
29	39.4307267300846	41.0	39.0	41.0	36.0	41.0
30	39.372285758918665	41.0	39.0	41.0	36.0	41.0
31	39.32975819513759	41.0	39.0	41.0	36.0	41.0
32	39.263683228230576	41.0	39.0	41.0	35.0	41.0
33	39.20752382283539	41.0	39.0	41.0	35.0	41.0
34	39.16376709118863	41.0	39.0	41.0	35.0	41.0
35	39.074625699885715	41.0	39.0	41.0	35.0	41.0
36	39.02085885535284	41.0	39.0	41.0	35.0	41.0
37	38.966047834773526	41.0	39.0	41.0	35.0	41.0
38	38.89774885510619	40.0	39.0	41.0	35.0	41.0
39	38.826226906854565	40.0	38.0	41.0	35.0	41.0
40	38.73512049133827	40.0	38.0	41.0	35.0	41.0
41	38.65663873975351	40.0	38.0	41.0	35.0	41.0
42	38.54468991260164	40.0	38.0	41.0	35.0	41.0
43	37.76677875800603	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	5.0
20	9.0
21	21.0
22	26.0
23	87.0
24	128.0
25	210.0
26	315.0
27	450.0
28	667.0
29	830.0
30	1153.0
31	1610.0
32	2039.0
33	2721.0
34	4263.0
35	6298.0
36	9978.0
37	19028.0
38	47223.0
39	146191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99168770092167	17.87226520427208	11.954993545840972	27.181053548965277
2	20.116010425316748	19.328356368240605	32.84468086855715	27.7109523378855
3	20.53779177320825	21.29749151093096	27.95185279584303	30.212863920017757
4	15.37035362213982	15.501903360273623	33.099969579123055	36.0277734384635
5	16.820689485064992	33.913522490894294	32.25147376816003	17.014314255880684
6	35.26519605021911	33.36265796245899	15.402007777878268	15.970138209443627
7	31.229085647101385	28.28648244222089	20.40747531386945	20.076956596808273
8	28.48668469994327	31.206475535859635	18.902052998100753	21.404786766096343
9	27.22668486438044	14.090210232925255	19.227227507050245	39.45587739564406
10	19.152408593486644	24.508949493122415	30.87719009759346	25.46145181579748
11	37.79958397395315	20.72771670763893	19.947051230401144	21.525648088006776
12	23.265393374826314	24.502783099147393	26.601001422381543	25.63082210364475
13	31.53987190344249	18.073700740789462	23.458195959778667	26.92823139598938
14	24.19076356401128	19.654764155985102	22.954607118485203	33.19986516151841
15	27.35905678837758	25.792381625790327	20.415697172502817	26.432864413329277
16	27.025660420794722	24.06126929053582	22.532414677662032	26.380655611007427
17	25.464740559250824	24.8871549902571	23.397354205891784	26.250750244600297
18	25.683030905966604	23.375155187581704	24.552114250947568	26.389699655504124
19	26.254450080985308	23.80145855772156	24.425086535062114	25.51900482623102
20	26.266371776003684	23.499305252945483	24.10237858370263	26.131944387348206
21	27.16337655290355	23.809269323423255	23.612766902085884	25.414587221587315
22	27.468818601132973	23.21112910784612	23.198796319896076	26.121255971124835
23	26.090424001249723	23.405576064525146	23.82489085482664	26.67910907939849
24	25.83965731293216	24.11594465044768	23.302802831608112	26.741595205012047
25	26.51179425620956	23.172897465200982	23.663742425612732	26.651565852976724
26	25.979840002630993	24.02632639134403	24.14307678393778	25.850756822087202
27	26.464929661999392	23.21729550182114	23.80104746478989	26.516727371389575
28	25.479539904790876	23.66703116906608	24.860433949698667	25.992994976444372
29	25.641099426936453	23.5609691926957	24.98335073626744	25.814580644100403
30	25.14449916548135	24.198574329712976	24.557869551990922	26.099056952814752
31	26.337079760250603	23.67813067822112	23.67360865597277	26.31118090555551
32	25.129699819941298	23.57042433012407	24.2343394147681	27.065536435166532
33	25.13709949271132	23.10958915372409	24.7761598987067	26.977151454857882
34	26.296792652947126	22.31700198146793	24.852212091065308	26.53399327451964
35	25.76936042161691	22.848956235046494	25.361145140470455	26.02053820286614
36	25.03103751634094	22.990372203540332	24.87564438817039	27.102945891948334
37	25.12517779769295	21.80642456033611	25.960518634842593	27.10787900712835
38	24.401243145025365	21.815879697764476	26.780649033520515	27.00222812368964
39	24.317791279896735	21.565113009446915	27.477451552698	26.639644157958347
40	24.264760291711546	21.457406661349864	27.868400930714397	26.409432116224195
41	23.111233525450764	21.701595862760737	28.703741767864045	26.483428843924457
42	23.030248217912142	22.102000378205496	28.521627599135062	26.3461238047473
43	22.348656137206376	21.317223971651032	28.950808619796593	27.383311271346
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	13.0
2	12.0
3	40.5
4	69.0
5	69.0
6	77.0
7	85.0
8	78.5
9	72.0
10	121.0
11	170.0
12	170.0
13	279.0
14	388.0
15	558.5
16	729.0
17	676.0
18	623.0
19	623.0
20	736.5
21	850.0
22	737.0
23	624.0
24	755.5
25	887.0
26	887.0
27	1175.5
28	1464.0
29	1981.0
30	2498.0
31	3109.5
32	3721.0
33	3721.0
34	4710.5
35	5700.0
36	6991.5
37	8283.0
38	9671.0
39	11059.0
40	11059.0
41	12299.5
42	13540.0
43	14766.0
44	15992.0
45	16556.0
46	17120.0
47	17120.0
48	18032.0
49	18944.0
50	19146.0
51	19348.0
52	19320.0
53	19292.0
54	19292.0
55	18637.5
56	17983.0
57	17255.0
58	16527.0
59	15953.0
60	15379.0
61	15379.0
62	14245.5
63	13112.0
64	12085.5
65	11059.0
66	9899.0
67	8739.0
68	8739.0
69	7454.5
70	6170.0
71	5394.0
72	4618.0
73	3691.0
74	2764.0
75	2764.0
76	2268.5
77	1773.0
78	1522.5
79	1272.0
80	1115.5
81	959.0
82	959.0
83	777.0
84	595.0
85	498.5
86	402.0
87	334.5
88	267.0
89	267.0
90	189.5
91	112.0
92	68.5
93	25.0
94	16.5
95	8.0
96	8.0
97	6.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	243254.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.17445317546267
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.7558981071931	59.62128921447779
2	5.550197515178668	7.456629661958774
3	1.7551453554562804	3.5370278848868604
4	0.9292859064209967	2.4969709042997854
5	0.6234989032547424	2.0941598940819017
6	0.4249531215636395	1.7127596139746035
7	0.29076936241923307	1.3672591044482933
8	0.23020463241900857	1.2371096240964228
9	0.19571232548800072	1.1832181599919154
>10	1.1621586677753755	14.61381989247246
>50	0.06684474036246886	3.110637138921231
>100	0.015331362468456162	1.5691189063900106
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	328	0.1348384815871476	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	326	0.13401629572381132	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	251	0.10318432584870137	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	4.110929316681329E-4	0.0
18	0.0	0.0	0.0	4.110929316681329E-4	0.0
19	0.0	0.0	0.0	4.110929316681329E-4	0.0
20	0.0	0.0	0.0	8.221858633362658E-4	0.0
21	0.0	0.0	0.0	8.221858633362658E-4	0.0
22	0.0	0.0	0.0	0.0012332787950043987	0.0
23	0.0	0.0	0.0	0.0020554646583406644	0.0
24	0.0	0.0	0.0	0.003288743453345063	0.0
25	0.0	0.0	0.0	0.00575530104335386	0.0
26	0.0	0.0	0.0	0.00986623036003519	0.0
27	0.0	0.0	0.0	0.04933115180017595	0.0
28	0.0	0.0	0.0	0.17307012423228396	0.0
29	0.0	0.0	0.0	0.30708641995609526	0.0
30	0.0	0.0	0.0	0.5035888412934628	0.0
31	0.0	0.0	0.0	1.04582041816373	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACTTT	45	1.3190272E-4	24.666666	24
TGCAAGA	45	1.3190272E-4	24.666666	2
TGGAGGT	40	0.0019268374	23.125	4
GGTATCA	185	0.0	23.0	1
CGATTTG	50	2.6936256E-4	22.199999	28
GAACCGC	50	2.6936256E-4	22.199999	6
ACCTCCG	60	3.710762E-5	21.583334	19
TATACAC	95	7.075869E-9	21.421053	36
GTGCAAG	45	0.003817273	20.555555	1
CGCGGTC	45	0.003817273	20.555555	10
AGAGTGT	45	0.003817273	20.555555	6
ATACACA	100	1.2772944E-8	20.349998	37
GCCTTTC	55	5.127091E-4	20.181818	7
GATTTGT	55	5.127091E-4	20.181818	29
ATCGTCT	55	5.127091E-4	20.181818	8
CCGACTT	55	5.127091E-4	20.181818	23
TCGTTCT	60	9.2083064E-4	18.5	30
TTCGTTC	60	9.2083064E-4	18.5	29
ATAGAAC	70	1.21419E-4	18.5	3
TGGCCGT	70	1.21419E-4	18.5	6
>>END_MODULE