FastQCFastQC Report
Fri 10 Feb 2017
ERR1631546.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631546.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140117
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.268347167010427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3700.26406503136664355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2980.2126794036412427No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1960.1398830976969247No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1830.13060513713539398No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1820.1298914478614301No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1780.12703669076557447No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1500.10705339109458524No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAG200.001836425237.05
CGCAAGA303.5835087E-430.8333322
CGCGCAA358.8304136E-426.42857221
GCGCAAA358.8304136E-426.42857222
CGCAAAT358.8304136E-426.42857223
GCGCGCA400.001922780323.12500220
TACTGCT400.001922780323.1250027
CAAGACG400.001922780323.1250024
GGGGGAC400.001922780323.12500223
GCAAGAC502.6857265E-422.23
TTATACA806.870796E-720.812537
GCGAAAG450.003809312820.55555718
AAGAATG450.003809312820.55555733
CCGCTTA450.003809312820.55555725
GCGCAAG450.003809312820.5555571
ACGGACC450.003809312820.5555578
AGCGAAA450.003809312820.55555717
CAGAGCG450.003809312820.55555714
AGAGCGA450.003809312820.55555715
CGCTTAT450.003809312820.55555726