##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631546.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	140117
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0350921016008	34.0	33.0	34.0	31.0	34.0
2	33.176616684627845	34.0	33.0	34.0	31.0	34.0
3	33.25818423174918	34.0	34.0	34.0	31.0	34.0
4	36.49604259297587	37.0	37.0	37.0	35.0	37.0
5	36.482689466660005	37.0	37.0	37.0	35.0	37.0
6	36.5290864063604	37.0	37.0	37.0	35.0	37.0
7	36.53188406831434	37.0	37.0	37.0	35.0	37.0
8	36.5273949627811	37.0	37.0	37.0	35.0	37.0
9	38.38366507989751	39.0	39.0	39.0	37.0	39.0
10	38.34076521763954	39.0	39.0	39.0	37.0	39.0
11	38.39996574291485	39.0	39.0	39.0	37.0	39.0
12	38.354232534239245	39.0	39.0	39.0	37.0	39.0
13	38.376314080375685	39.0	39.0	39.0	37.0	39.0
14	39.92472719227503	41.0	40.0	41.0	38.0	41.0
15	39.90808395840619	41.0	40.0	41.0	38.0	41.0
16	39.873548534439074	41.0	40.0	41.0	38.0	41.0
17	39.85649849768408	41.0	40.0	41.0	38.0	41.0
18	39.85208789797098	41.0	40.0	41.0	38.0	41.0
19	39.85884653539542	41.0	40.0	41.0	38.0	41.0
20	39.83248285361519	41.0	40.0	41.0	38.0	41.0
21	39.80648315336469	41.0	40.0	41.0	38.0	41.0
22	39.74588379711241	41.0	40.0	41.0	38.0	41.0
23	39.69056574148747	41.0	40.0	41.0	37.0	41.0
24	39.66465882084258	41.0	40.0	41.0	37.0	41.0
25	39.65243332357958	41.0	40.0	41.0	37.0	41.0
26	39.55962517039331	41.0	40.0	41.0	37.0	41.0
27	39.49624956286532	41.0	40.0	41.0	37.0	41.0
28	39.42406702969661	41.0	39.0	41.0	36.0	41.0
29	39.36939843131098	41.0	39.0	41.0	36.0	41.0
30	39.319604330666515	41.0	39.0	41.0	36.0	41.0
31	39.257334941513164	41.0	39.0	41.0	35.0	41.0
32	39.18451722489063	41.0	39.0	41.0	35.0	41.0
33	39.10125823419	41.0	39.0	41.0	35.0	41.0
34	39.03401443079712	41.0	39.0	41.0	35.0	41.0
35	38.950805398345665	41.0	39.0	41.0	35.0	41.0
36	38.88533868124496	40.0	39.0	41.0	35.0	41.0
37	38.82039295731425	40.0	38.0	41.0	35.0	41.0
38	38.73503572014816	40.0	38.0	41.0	35.0	41.0
39	38.63568303631965	40.0	38.0	41.0	35.0	41.0
40	38.52650285118865	40.0	38.0	41.0	35.0	41.0
41	38.419171121277216	40.0	38.0	41.0	35.0	41.0
42	38.276283391736904	40.0	37.0	41.0	35.0	41.0
43	37.45167253081353	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	3.0
19	4.0
20	6.0
21	16.0
22	26.0
23	54.0
24	76.0
25	116.0
26	184.0
27	259.0
28	415.0
29	572.0
30	717.0
31	899.0
32	1226.0
33	1660.0
34	2500.0
35	3791.0
36	6106.0
37	11989.0
38	27611.0
39	81885.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.88546714531427	16.773125316699616	11.959291163813099	27.38211637417301
2	20.143166068357157	18.6950905314844	32.66555806932778	28.496185330830663
3	20.4943011911474	20.878266020539975	27.887408380139455	30.740024408173173
4	14.802629231285284	14.888985633434915	32.445741772946896	37.86264336233291
5	16.934419092615457	34.43907591512807	31.73562094535281	16.890884046903658
6	36.366036954830605	32.42147633763212	15.559853550960984	15.65263315657629
7	31.967569959391078	28.52687396961111	19.178258169957964	20.327297901039845
8	30.232591334384836	30.316092979438615	18.45671831398046	20.994597372196093
9	28.100087783780697	13.441623785836123	18.062761834752386	40.39552659563079
10	19.24391758316264	24.307542981936525	29.932128150046033	26.5164112848548
11	38.68195864884347	20.602781960789912	19.01482332622023	21.70043606414639
12	24.10699629595267	23.937851938023226	26.400793622472644	25.554358143551458
13	32.088183446690984	17.517503229443964	23.14494315464933	27.249370169215727
14	24.26472162549869	18.98484837671375	22.70031473696982	34.05011526081774
15	27.997316528329897	25.333828157896615	19.96403006059222	26.70482525318127
16	27.62334334877281	23.645953024971988	22.20858282720869	26.52212079904651
17	25.928331323108544	24.597300827165867	23.008628503322225	26.46573934640336
18	26.72124010648244	22.304217189919854	24.33823162071697	26.636311082880738
19	26.59705817281272	23.816524761449358	23.656658364081444	25.929758701656475
20	27.090931150395743	22.32205942176895	23.90644960996881	26.680559817866495
21	28.515454941227684	22.870172784173228	23.208461500032115	25.40591077456697
22	28.04727477750737	22.234989330345353	23.06857840233519	26.649157489812087
23	26.52069342049858	22.49049009042443	23.52533953767209	27.463476951404896
24	26.198819557940862	23.32479285168823	23.723031466560087	26.753356123810818
25	26.400079933198683	22.505477565177674	23.64309826787613	27.45134423374751
26	26.766916220016128	23.491796141795785	23.298386348551567	26.442901289636517
27	27.04668241540998	22.42268960939786	23.41757245730354	27.113055517888622
28	25.882655209574857	22.957956564870788	24.166232505691674	26.99315571986269
29	25.4665743628539	23.341207704989404	24.49167481461921	26.700543117537485
30	25.008385848969077	23.74729690187486	24.974128763818808	26.270188485337254
31	26.48215419970453	23.5745840975756	23.23344062462085	26.709821078099015
32	25.729212015672616	23.167781211416173	23.94784358785872	27.155163185052494
33	25.331687090074723	22.47978475131497	24.754312467437927	27.434215691172376
34	26.277325378076892	22.72814861865441	23.973536401721418	27.020989601547278
35	26.31015508467923	22.976512485993847	24.662960240370545	26.050372188956374
36	24.778577902752698	23.577438854671453	24.550911024358214	27.093072218217635
37	25.8362654067672	22.287802336618682	24.93416216447683	26.941770092137286
38	24.939871678668542	21.587673158860095	26.133160144736184	27.339295017735175
39	25.540084358072185	21.46063646809452	26.033243646381237	26.96603552745206
40	24.872071197641972	21.659042086256484	26.496427985183814	26.97245873091773
41	23.42114090367336	22.103670503935994	27.645467716265692	26.829720876124952
42	24.35250540619625	21.906692264321958	27.495592968733273	26.245209360748518
43	23.2384364495386	21.32289443821949	27.626911795142632	27.811757317099286
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	5.0
2	6.0
3	21.5
4	37.0
5	37.0
6	51.5
7	66.0
8	68.0
9	70.0
10	100.5
11	131.0
12	131.0
13	225.5
14	320.0
15	534.0
16	748.0
17	701.0
18	654.0
19	654.0
20	747.5
21	841.0
22	669.0
23	497.0
24	555.5
25	614.0
26	614.0
27	755.0
28	896.0
29	1120.5
30	1345.0
31	1604.0
32	1863.0
33	1863.0
34	2304.0
35	2745.0
36	3431.0
37	4117.0
38	4651.5
39	5186.0
40	5186.0
41	5736.0
42	6286.0
43	6757.5
44	7229.0
45	7916.5
46	8604.0
47	8604.0
48	9581.0
49	10558.0
50	10795.0
51	11032.0
52	10987.0
53	10942.0
54	10942.0
55	10702.0
56	10462.0
57	10198.0
58	9934.0
59	9861.0
60	9788.0
61	9788.0
62	9234.0
63	8680.0
64	8088.5
65	7497.0
66	6884.0
67	6271.0
68	6271.0
69	5356.0
70	4441.0
71	3761.0
72	3081.0
73	2458.5
74	1836.0
75	1836.0
76	1484.0
77	1132.0
78	934.0
79	736.0
80	660.5
81	585.0
82	585.0
83	467.0
84	349.0
85	311.0
86	273.0
87	220.5
88	168.0
89	168.0
90	124.0
91	80.0
92	44.5
93	9.0
94	6.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	140117.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.09870332528702
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.75723910961024	67.07335199620822
2	4.404907789876889	6.439860954149128
3	1.289984415493135	2.8288856424712936
4	0.6359678663143423	1.8595370553651163
5	0.4019791616558308	1.4692077740413585
6	0.25998651082392515	1.1402806093976778
7	0.18699029788390203	0.956812381680568
8	0.11399408533504946	0.6666255859795386
9	0.09199522676145541	0.6052258609540321
>10	0.7679601538347103	11.130162058872548
>50	0.06999636818805952	3.4544654817253337
>100	0.018999014222473295	2.3755845991551703
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	376	0.268347167010427	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	370	0.26406503136664355	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	298	0.2126794036412427	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	196	0.1398830976969247	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	183	0.13060513713539398	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	182	0.1298914478614301	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	178	0.12703669076557447	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	150	0.10705339109458524	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	7.136892739639016E-4	0.0
14	0.0	0.0	0.0	7.136892739639016E-4	0.0
15	0.0	0.0	0.0	0.0014273785479278032	0.0
16	0.0	0.0	0.0	0.0014273785479278032	0.0
17	0.0	0.0	0.0	0.004995824917747312	0.0
18	0.0	0.0	0.0	0.005709514191711213	0.0
19	0.0	0.0	0.0	0.0064232034656751145	0.0
20	0.0	0.0	0.0	0.0064232034656751145	0.0
21	0.0	0.0	0.0	0.007850582013602917	0.0
22	0.0	0.0	0.0	0.011419028383422425	0.0
23	0.0	0.0	0.0	0.015701164027205835	0.0
24	0.0	0.0	0.0	0.02212436749288095	0.0
25	0.0	0.0	0.0	0.02283805676684485	0.0
26	0.0	0.0	0.0	0.029974949506483866	0.0
27	0.0	0.0	0.0	0.054240384821256524	0.0
28	0.0	0.0	0.0	0.14202416551881641	0.0
29	0.0	0.0	0.0	0.2583555171749324	0.0
30	0.0	0.0	0.0	0.42749987510437704	0.0
31	0.0	0.0	0.0	0.8799788747974907	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACCAG	20	0.0018364252	37.0	5
CGCAAGA	30	3.5835087E-4	30.833332	2
CGCGCAA	35	8.8304136E-4	26.428572	21
GCGCAAA	35	8.8304136E-4	26.428572	22
CGCAAAT	35	8.8304136E-4	26.428572	23
GCGCGCA	40	0.0019227803	23.125002	20
TACTGCT	40	0.0019227803	23.125002	7
CAAGACG	40	0.0019227803	23.125002	4
GGGGGAC	40	0.0019227803	23.125002	23
GCAAGAC	50	2.6857265E-4	22.2	3
TTATACA	80	6.870796E-7	20.8125	37
GCGAAAG	45	0.0038093128	20.555557	18
AAGAATG	45	0.0038093128	20.555557	33
CCGCTTA	45	0.0038093128	20.555557	25
GCGCAAG	45	0.0038093128	20.555557	1
ACGGACC	45	0.0038093128	20.555557	8
AGCGAAA	45	0.0038093128	20.555557	17
CAGAGCG	45	0.0038093128	20.555557	14
AGAGCGA	45	0.0038093128	20.555557	15
CGCTTAT	45	0.0038093128	20.555557	26
>>END_MODULE