FastQCFastQC Report
Fri 10 Feb 2017
ERR1631545.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631545.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146791
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2710.18461622306544678No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC2340.1594103180712714No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC2130.14510426388538805No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC2100.1430605418588333No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1920.13079820969950476No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC1490.10150486065221984No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTCG200.001836709837.09
CGAGCCG405.9054262E-527.7515
ACTCTTT358.832423E-426.4285728
ATACCGC358.832423E-426.42857227
TACCGCA358.832423E-426.42857228
CTGACTC451.3155438E-424.66666633
TGGATAC451.3155438E-424.66666624
ACCGCAG400.001923214223.12529
GGATACC502.6865694E-422.225
GTATCAA1151.3096724E-1020.9130441
GTATTGC450.003810163120.55555528
CGCAGCT450.003810163120.55555531
GGTATCA555.113771E-420.1818181
TGACTCT555.113771E-420.18181834
CCGAGCC656.849839E-519.92307714
AAGCAGG851.2302335E-619.5882346
CTAGATA500.007006389618.53
TAGAACT500.007006389618.54
TTCGTAT500.007006389618.525
GCATTCG609.184582E-418.522
Produced by FastQC (version 0.11.5)