##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631545.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	146791
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02538983997657	34.0	33.0	34.0	31.0	34.0
2	33.180392530877235	34.0	33.0	34.0	31.0	34.0
3	33.265956359722324	34.0	34.0	34.0	31.0	34.0
4	36.505201272557585	37.0	37.0	37.0	35.0	37.0
5	36.49032978861102	37.0	37.0	37.0	35.0	37.0
6	36.53307082859303	37.0	37.0	37.0	35.0	37.0
7	36.54125934151276	37.0	37.0	37.0	35.0	37.0
8	36.53045486439904	37.0	37.0	37.0	35.0	37.0
9	38.391686138795976	39.0	39.0	39.0	37.0	39.0
10	38.33666914184112	39.0	39.0	39.0	37.0	39.0
11	38.409085025648714	39.0	39.0	39.0	37.0	39.0
12	38.35630249810956	39.0	39.0	39.0	37.0	39.0
13	38.38426742783958	39.0	39.0	39.0	37.0	39.0
14	39.925683454707716	41.0	40.0	41.0	38.0	41.0
15	39.92436184779721	41.0	40.0	41.0	38.0	41.0
16	39.9036453188547	41.0	40.0	41.0	38.0	41.0
17	39.899591936835364	41.0	40.0	41.0	38.0	41.0
18	39.86963097192607	41.0	40.0	41.0	38.0	41.0
19	39.866136207260666	41.0	40.0	41.0	38.0	41.0
20	39.83796009292123	41.0	40.0	41.0	38.0	41.0
21	39.813094808264815	41.0	40.0	41.0	38.0	41.0
22	39.7587249899517	41.0	40.0	41.0	38.0	41.0
23	39.706140022208444	41.0	40.0	41.0	37.0	41.0
24	39.673760652901066	41.0	40.0	41.0	37.0	41.0
25	39.657519875196705	41.0	40.0	41.0	37.0	41.0
26	39.58391863261372	41.0	40.0	41.0	37.0	41.0
27	39.52434413553964	41.0	40.0	41.0	37.0	41.0
28	39.45247324427247	41.0	39.0	41.0	37.0	41.0
29	39.40413921834445	41.0	39.0	41.0	36.0	41.0
30	39.343168177885566	41.0	39.0	41.0	36.0	41.0
31	39.313895266058545	41.0	39.0	41.0	36.0	41.0
32	39.23786880667071	41.0	39.0	41.0	35.0	41.0
33	39.16917249695145	41.0	39.0	41.0	35.0	41.0
34	39.118140757948375	41.0	39.0	41.0	35.0	41.0
35	39.03524739255131	41.0	39.0	41.0	35.0	41.0
36	38.98327554141603	40.0	39.0	41.0	35.0	41.0
37	38.92486596589709	40.0	39.0	41.0	35.0	41.0
38	38.84307621039437	40.0	38.0	41.0	35.0	41.0
39	38.76696800212547	40.0	38.0	41.0	35.0	41.0
40	38.690430612231	40.0	38.0	41.0	35.0	41.0
41	38.60227125641218	40.0	38.0	41.0	35.0	41.0
42	38.47757015075856	40.0	37.0	41.0	35.0	41.0
43	37.66210462494295	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	0.0
19	4.0
20	4.0
21	15.0
22	22.0
23	44.0
24	74.0
25	124.0
26	172.0
27	244.0
28	383.0
29	500.0
30	663.0
31	858.0
32	1193.0
33	1678.0
34	2523.0
35	3724.0
36	6337.0
37	12072.0
38	29844.0
39	86311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.74723245975571	16.90089991893236	11.34946965413411	28.00239796717782
2	20.572787159975746	18.729349892023354	31.98424971558202	28.713613232418883
3	20.96518178907426	19.82682862028326	27.79325707979372	31.414732510848758
4	15.214148006349163	14.20931800995974	31.749221682528223	38.82731230116288
5	17.43499260853867	33.78817502435435	31.617742232153194	17.15909013495378
6	38.18353986279813	31.898413390466718	14.517238795293991	15.400807951441164
7	32.67843396393511	27.736714103725706	19.067926507755924	20.516925424583253
8	29.790654740413242	29.92690287551689	18.652369695689792	21.63007268838008
9	27.91724288273804	13.219475308431717	17.855318105333435	41.007963703496806
10	19.086320005994917	23.745324985864254	29.80700451662568	27.361350491515147
11	40.04264566628744	19.363584960930847	19.02296462317172	21.57080474960999
12	24.82032277183206	22.80316913162251	25.701848205952682	26.67465989059275
13	32.61303485908537	16.77895783801459	22.953723320912044	27.654283981987998
14	24.87141582249593	18.401673127099073	21.540830159887186	35.186080890517815
15	27.915880401387007	24.774679646572338	19.488933245226207	27.820506706814452
16	28.438391999509506	23.06544679169704	21.053743076891635	27.442418131901817
17	27.472392721624622	23.31546211961224	21.816051392796563	27.39609376596658
18	27.657690185365585	21.541511400562705	23.277993882458734	27.52280453161297
19	27.59024735848928	22.60356561369566	22.205039818517484	27.601147209297572
20	27.58615991443617	21.831038687657962	22.28065753350001	28.302143864405853
21	29.210918925547208	22.402599614417777	22.134872028939103	26.25160943109591
22	29.442540755223412	21.67639705431532	21.436600336532894	27.444461853928377
23	27.661777629418697	21.501999441382647	22.509554400474144	28.326668528724515
24	27.241452132623934	22.568141098568713	22.123290937455295	28.06711583135206
25	27.716276883460157	21.521074180297155	22.376031228072566	28.38661770817012
26	27.755107601964696	22.671689681247486	22.200271133788856	27.372931582998955
27	28.288519050895488	21.635522613784225	22.330388102812844	27.745570232507443
28	27.088854221307844	22.154628008529134	22.65193370165746	28.104584068505563
29	26.531599348733913	22.211170984597146	23.288212492591505	27.969017174077432
30	25.808802992009046	23.34271174663297	23.41219829553583	27.436286965822156
31	27.87568720153143	22.301094753765557	21.90733764331601	27.915880401387007
32	26.731202866660762	22.215939669325778	22.542935193574536	28.509922270438924
33	26.400801139034407	21.604185542710383	23.1833014285617	28.81171188969351
34	27.462855352167363	21.58579204447139	23.414923258237902	27.536429345123338
35	27.16038449223726	21.20838471023428	24.26306789925813	27.36816289827033
36	25.963444625351688	22.166209100012942	23.75894979937462	28.111396475260747
37	26.813632988398474	20.754678420339122	24.56962620324134	27.862062388021062
38	25.461370247494735	20.187886178307934	25.77474095823313	28.576002615964196
39	26.043831025062847	19.807753881368747	25.99137549304794	28.157039600520466
40	25.25767928551478	20.647042393607236	26.622204358577843	27.47307396230014
41	24.376153851394157	20.370458679346825	27.95130491651395	27.30208255274506
42	24.523301837306104	20.517606665258768	28.107309031207635	26.85178246622749
43	22.961216968342747	19.684449319099944	28.1509084344408	29.20342527811651
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	4.5
2	3.0
3	9.5
4	16.0
5	16.0
6	20.5
7	25.0
8	21.0
9	17.0
10	19.5
11	22.0
12	22.0
13	47.0
14	72.0
15	126.0
16	180.0
17	177.0
18	174.0
19	174.0
20	205.5
21	237.0
22	219.5
23	202.0
24	259.5
25	317.0
26	317.0
27	388.5
28	460.0
29	669.5
30	879.0
31	1095.0
32	1311.0
33	1311.0
34	1724.5
35	2138.0
36	2804.0
37	3470.0
38	4161.5
39	4853.0
40	4853.0
41	5431.0
42	6009.0
43	6600.0
44	7191.0
45	7994.5
46	8798.0
47	8798.0
48	9565.5
49	10333.0
50	10863.0
51	11393.0
52	12032.0
53	12671.0
54	12671.0
55	12550.5
56	12430.0
57	12236.0
58	12042.0
59	11852.5
60	11663.0
61	11663.0
62	11054.5
63	10446.0
64	9861.5
65	9277.0
66	8281.5
67	7286.0
68	7286.0
69	6061.5
70	4837.0
71	4062.0
72	3287.0
73	2487.5
74	1688.0
75	1688.0
76	1369.5
77	1051.0
78	860.0
79	669.0
80	592.5
81	516.0
82	516.0
83	422.5
84	329.0
85	280.0
86	231.0
87	191.0
88	151.0
89	151.0
90	119.0
91	87.0
92	52.0
93	17.0
94	10.5
95	4.0
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	146791.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.92372148156223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.85869011991286	59.46754228801493
2	5.7116390806001744	7.644882860665844
3	1.775280441377064	3.564251214311504
4	0.9202141737413221	2.4633662826740057
5	0.5680083063580285	1.9006614846959282
6	0.36747490787678905	1.4755673031725378
7	0.2738247928500173	1.282776192000872
8	0.18526435798774404	0.9918864235545777
9	0.16897738146134897	1.0177735692242713
>10	1.046438241820884	13.209937938974461
>50	0.09466805105967141	4.28772881171189
>100	0.029520144954091082	2.6936256309991755
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	271	0.18461622306544678	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	234	0.1594103180712714	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	213	0.14510426388538805	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	210	0.1430605418588333	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	192	0.13079820969950476	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	149	0.10150486065221984	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	6.812406755182538E-4	0.0
15	0.0	0.0	0.0	6.812406755182538E-4	0.0
16	0.0	0.0	0.0	0.0013624813510365076	0.0
17	0.0	0.0	0.0	0.0020437220265547614	0.0
18	0.0	0.0	0.0	0.0020437220265547614	0.0
19	0.0	0.0	0.0	0.0020437220265547614	0.0
20	0.0	0.0	0.0	0.0020437220265547614	0.0
21	0.0	0.0	0.0	0.0020437220265547614	0.0
22	0.0	0.0	0.0	0.003406203377591269	0.0
23	0.0	0.0	0.0	0.006131166079664284	0.0
24	0.0	0.0	0.0	0.010218610132773807	0.0
25	0.0	0.0	0.0	0.010899850808292061	0.0
26	0.0	0.0	0.0	0.019074738914511107	0.0
27	0.0	0.0	0.0	0.04973056931283253	0.0
28	0.0	0.0	0.0	0.16145404009782616	0.0
29	0.0	0.0	0.0	0.3242705615466888	0.0
30	0.0	0.0	0.0	0.5374988929839023	0.0
31	0.0	0.0	0.0	1.1335844840623743	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAGTCG	20	0.0018367098	37.0	9
CGAGCCG	40	5.9054262E-5	27.75	15
ACTCTTT	35	8.832423E-4	26.428572	8
ATACCGC	35	8.832423E-4	26.428572	27
TACCGCA	35	8.832423E-4	26.428572	28
CTGACTC	45	1.3155438E-4	24.666666	33
TGGATAC	45	1.3155438E-4	24.666666	24
ACCGCAG	40	0.0019232142	23.125	29
GGATACC	50	2.6865694E-4	22.2	25
GTATCAA	115	1.3096724E-10	20.913044	1
GTATTGC	45	0.0038101631	20.555555	28
CGCAGCT	45	0.0038101631	20.555555	31
GGTATCA	55	5.113771E-4	20.181818	1
TGACTCT	55	5.113771E-4	20.181818	34
CCGAGCC	65	6.849839E-5	19.923077	14
AAGCAGG	85	1.2302335E-6	19.588234	6
CTAGATA	50	0.0070063896	18.5	3
TAGAACT	50	0.0070063896	18.5	4
TTCGTAT	50	0.0070063896	18.5	25
GCATTCG	60	9.184582E-4	18.5	22
>>END_MODULE