FastQCFastQC Report
Fri 10 Feb 2017
ERR1631544.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631544.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40736
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6321.551453260015711No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5201.276512175962294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4541.1144933228593872No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2510.6161626080125687No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT2400.589159465828751No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.5670659858601729No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT2040.5007855459544384No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT2030.4983307148468185No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1860.456598586017282No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1840.4516889238020424No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1750.4295954438334642No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1390.3412215239591516No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1360.33385703063629224No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA1320.324037706205813No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA1300.3191280439905735No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1220.2994893951296151No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT1060.26021209740769835No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT960.2356637863315004No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT950.23320895522388058No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT940.23075412411626078No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA880.216025137470542No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT860.21111547525530244No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC850.20866064414768265No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA850.20866064414768265No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA820.20129615082482322No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT790.19393165750196387No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC740.1816575019638649No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC720.1767478397486253No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT710.1742930086410055No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.1718381775333857No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC700.1718381775333857No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT700.1718381775333857No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT660.1620188531029065No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.1620188531029065No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT660.1620188531029065No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA640.15710919088766695No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG640.15710919088766695No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA630.15465435978004713No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC630.15465435978004713No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA630.15465435978004713No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATA580.14238020424194817No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG560.13747054202670855No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT550.13501571091908876No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT550.13501571091908876No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA540.13256087981146897No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.12765121759622938No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA520.12765121759622938No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA510.1251963864886096No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACCTGTCT500.12274155538098978No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT500.12274155538098978No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT490.12028672427336999No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT480.1178318931657502No Hit
TATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATACA480.1178318931657502No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT470.11537706205813039No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT470.11537706205813039No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT470.11537706205813039No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.1129222309505106No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACA450.11046739984289082No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGCTGTCTCTT440.108012568735271No Hit
CAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCTT440.108012568735271No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGCTGTCTCTTATA430.10555773762765122No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA420.10310290652003143ABI Solid3 Adapter B (100% over 21bp)
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT410.10064807541241161No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.10064807541241161No Hit
CGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCTCT410.10064807541241161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGAC250.00543504529.630
ATTGACT250.00543504529.631
CGTTGAA358.7226514E-426.4285722
ACAGTGG551.8448947E-523.54545425
GGAACAG652.5789086E-622.7692322
GGTATCA5900.022.2627121
GGGAACA1106.730261E-1121.86363621
AACAGTG603.6139456E-521.58333224
TGGGCAG2600.021.3461536
GAAGCAT704.9054834E-621.14285923
GAACAGT704.9054834E-621.14285923
TGGGAAC1400.021.14285920
GCATGGT450.003763702520.55555526
GACTGTC450.003763702520.55555534
AGCATGG555.0269323E-420.18181825
GGGCAGT2700.019.870377
TGTTCCC758.91122E-619.7333345
AAGCATG758.91122E-619.73333424
GGCAGTC2750.019.5090928
GCAGTCG2800.019.1607159
Produced by FastQC (version 0.11.5)