##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631544.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40736
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89920463472113	34.0	33.0	34.0	31.0	34.0
2	32.98924783974863	34.0	33.0	34.0	31.0	34.0
3	32.936959937156324	34.0	33.0	34.0	31.0	34.0
4	36.312917321288296	37.0	37.0	37.0	35.0	37.0
5	36.35010801256873	37.0	37.0	37.0	35.0	37.0
6	36.45480655930872	37.0	37.0	37.0	35.0	37.0
7	36.458783385703065	37.0	37.0	37.0	35.0	37.0
8	36.49351924587588	37.0	37.0	37.0	35.0	37.0
9	38.30955420267085	39.0	39.0	39.0	37.0	39.0
10	38.278770620581305	39.0	39.0	39.0	37.0	39.0
11	38.32295758051846	39.0	39.0	39.0	37.0	39.0
12	38.30918597800471	39.0	39.0	39.0	37.0	39.0
13	38.31593676355067	39.0	39.0	39.0	37.0	39.0
14	39.699847800471325	41.0	40.0	41.0	37.0	41.0
15	39.63059701492537	41.0	39.0	41.0	37.0	41.0
16	39.476482717989	41.0	39.0	41.0	37.0	41.0
17	39.522731736056556	41.0	39.0	41.0	37.0	41.0
18	39.699725058915945	41.0	40.0	41.0	38.0	41.0
19	39.78007168106834	41.0	40.0	41.0	38.0	41.0
20	39.82563334642577	41.0	40.0	41.0	38.0	41.0
21	39.8196190102121	41.0	40.0	41.0	38.0	41.0
22	39.747643362136685	41.0	40.0	41.0	37.0	41.0
23	39.692876080125686	41.0	40.0	41.0	37.0	41.0
24	39.63280636292223	41.0	40.0	41.0	37.0	41.0
25	39.63152985074627	41.0	40.0	41.0	37.0	41.0
26	39.537632560879814	41.0	40.0	41.0	37.0	41.0
27	39.42748428908091	41.0	39.0	41.0	36.0	41.0
28	39.30368715632365	41.0	39.0	41.0	35.0	41.0
29	39.157035545954436	41.0	39.0	41.0	35.0	41.0
30	38.98676846032993	40.0	38.0	41.0	35.0	41.0
31	38.81318735271013	40.0	38.0	41.0	35.0	41.0
32	38.65551355066771	40.0	38.0	41.0	35.0	41.0
33	38.55665750196386	40.0	38.0	41.0	35.0	41.0
34	38.50498330714847	40.0	38.0	41.0	35.0	41.0
35	38.39650432050275	40.0	38.0	41.0	35.0	41.0
36	38.30582285938728	40.0	37.0	41.0	35.0	41.0
37	38.14392674783975	40.0	37.0	41.0	35.0	41.0
38	38.0009328358209	40.0	37.0	41.0	35.0	41.0
39	37.81141987431265	40.0	36.0	41.0	34.0	41.0
40	37.64481048703849	40.0	36.0	41.0	34.0	41.0
41	37.42242733699921	40.0	35.0	41.0	33.0	41.0
42	37.21818538884525	40.0	35.0	41.0	33.0	41.0
43	36.4027395915161	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	3.0
21	3.0
22	6.0
23	11.0
24	36.0
25	65.0
26	68.0
27	115.0
28	169.0
29	236.0
30	344.0
31	432.0
32	541.0
33	677.0
34	941.0
35	1262.0
36	2045.0
37	4877.0
38	7819.0
39	21084.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.843283582089555	13.35182639434407	17.822073841319718	24.982816182246662
2	20.630400628436764	19.901315789473685	35.27592301649646	24.192360565593088
3	17.689512961508246	23.885506677140615	33.100942655145325	25.324037706205814
4	11.675176747839748	21.207285938727416	32.42095443833464	34.696582875098194
5	12.993421052631579	38.74705420267085	32.20001963864886	16.059505106048704
6	25.866555380989787	32.035545954438334	21.622152395915162	20.475746268656717
7	27.85987824037706	33.920856245090334	18.330223880597014	19.889041633935587
8	33.86439512961508	29.082384131971722	17.79261586802828	19.260604870384917
9	31.323644933228596	12.4877258444619	15.573448546739984	40.615180675569526
10	21.590239591516102	26.134131971720347	20.64512961508248	31.63049882168107
11	30.80076590730558	27.702769049489394	13.980263157894738	27.51620188531029
12	24.86252945797329	33.12549096622152	22.974764336213667	19.037215239591514
13	35.77916339355852	18.666535742340926	25.0122741555381	20.54202670856245
14	24.234092694422625	22.57708169677926	24.891987431264727	28.296838177533385
15	33.94294972505892	22.805380989787903	20.92989002356638	22.3217792615868
16	22.159760408483898	26.627553024351926	27.663491751767477	23.549194815396703
17	17.22064021995287	32.649253731343286	25.36576983503535	24.7643362136685
18	23.0533189316575	21.1680086410055	29.082384131971722	26.696288295365278
19	21.90200314218382	31.085526315789476	29.178122545168893	17.834347996857815
20	26.18322859387274	20.6721327572663	33.120581304006286	20.024057344854672
21	33.432344854674	18.907109190887667	27.381186174391203	20.27935978004713
22	29.877749410840533	20.853790259230166	23.171150824823254	26.097309505106047
23	25.03191280439906	24.813432835820894	23.81922623723488	26.335428122545167
24	21.651610369206598	25.959838963079342	30.74921445404556	21.6393362136685
25	20.82187745483111	24.337195600942653	27.336999214454043	27.503927729772194
26	28.036626080125686	21.357030636292222	25.419776119402986	25.186567164179102
27	23.956696779261588	22.574626865671643	25.387863315003926	28.080813040062846
28	21.798900235663787	22.903574234092694	30.466908876669287	24.830616653574232
29	22.73173605655931	22.71946190102121	32.72780832678712	21.820993715632365
30	21.65897486252946	24.617046347211314	33.20650039277297	20.51747839748625
31	21.74980361351139	26.116948153967005	28.176551453260018	23.956696779261588
32	19.48399450117832	25.400137470542028	33.881578947368425	21.234289080911235
33	21.51904948939513	22.57708169677926	34.90278868813826	21.001080125687352
34	23.66702670856245	24.835526315789476	26.9466810683425	24.55076590730558
35	20.64021995286724	25.94756480754124	28.746072270227806	24.666142969363708
36	16.643754909662213	24.86252945797329	30.606834249803615	27.88688138256088
37	18.344952867242732	24.845345640219954	32.84564021995286	23.96406127258445
38	19.115769835035348	19.86940298507463	33.77602120974077	27.238805970149254
39	19.22378240377062	20.281814611154754	36.89365671641791	23.600746268656717
40	18.443146111547527	20.05351531814611	33.84721131186174	27.65612725844462
41	14.365671641791044	23.647388059701495	37.465632364493324	24.52130793401414
42	14.763354281225451	22.01247054202671	35.43303220738413	27.791142969363708
43	12.401806755695208	26.055577376276513	37.38462293794187	24.15799293008641
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	7.5
2	13.0
3	33.0
4	53.0
5	53.0
6	84.0
7	115.0
8	111.5
9	108.0
10	141.5
11	175.0
12	175.0
13	367.5
14	560.0
15	883.5
16	1207.0
17	1040.0
18	873.0
19	873.0
20	954.0
21	1035.0
22	718.5
23	402.0
24	369.5
25	337.0
26	337.0
27	301.0
28	265.0
29	304.5
30	344.0
31	327.0
32	310.0
33	310.0
34	359.5
35	409.0
36	447.5
37	486.0
38	645.5
39	805.0
40	805.0
41	1126.5
42	1448.0
43	1966.0
44	2484.0
45	3507.5
46	4531.0
47	4531.0
48	5625.5
49	6720.0
50	6010.5
51	5301.0
52	4159.5
53	3018.0
54	3018.0
55	2328.0
56	1638.0
57	1534.0
58	1430.0
59	1381.5
60	1333.0
61	1333.0
62	1243.5
63	1154.0
64	1106.0
65	1058.0
66	1013.5
67	969.0
68	969.0
69	835.5
70	702.0
71	639.5
72	577.0
73	485.0
74	393.0
75	393.0
76	306.5
77	220.0
78	179.0
79	138.0
80	105.5
81	73.0
82	73.0
83	47.5
84	22.0
85	19.0
86	16.0
87	12.5
88	9.0
89	9.0
90	6.0
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	40736.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.13599764336214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.53737192309262	53.24283189316576
2	5.572110870718864	6.701688923802042
3	1.8573702902396212	3.350844461901021
4	1.06951871657754	2.5726630007855458
5	0.5959913458790872	1.7920267085624508
6	0.38780258807200885	1.3992537313432836
7	0.28983140792750134	1.2200510604870385
8	0.2857492754214802	1.3747054202670856
9	0.15512103522880352	0.8395522388059702
>10	1.0531901865534554	12.26188138256088
>50	0.1265461076866555	5.314709347996858
>100	0.06123198759031718	7.1018263943440685
>500	0.008164265012042291	2.8279654359780046
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	632	1.551453260015711	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	520	1.276512175962294	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	454	1.1144933228593872	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	251	0.6161626080125687	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	240	0.589159465828751	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	231	0.5670659858601729	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	204	0.5007855459544384	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	203	0.4983307148468185	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	186	0.456598586017282	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	184	0.4516889238020424	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	175	0.4295954438334642	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	139	0.3412215239591516	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	136	0.33385703063629224	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	132	0.324037706205813	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	130	0.3191280439905735	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	122	0.2994893951296151	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT	106	0.26021209740769835	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	96	0.2356637863315004	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	95	0.23320895522388058	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	94	0.23075412411626078	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	88	0.216025137470542	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	86	0.21111547525530244	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC	85	0.20866064414768265	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	85	0.20866064414768265	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	82	0.20129615082482322	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT	79	0.19393165750196387	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC	74	0.1816575019638649	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	72	0.1767478397486253	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT	71	0.1742930086410055	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70	0.1718381775333857	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	70	0.1718381775333857	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	70	0.1718381775333857	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	66	0.1620188531029065	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	66	0.1620188531029065	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT	66	0.1620188531029065	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	64	0.15710919088766695	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	64	0.15710919088766695	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	63	0.15465435978004713	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	63	0.15465435978004713	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	63	0.15465435978004713	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATA	58	0.14238020424194817	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	56	0.13747054202670855	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT	55	0.13501571091908876	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	55	0.13501571091908876	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA	54	0.13256087981146897	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52	0.12765121759622938	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	52	0.12765121759622938	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA	51	0.1251963864886096	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACCTGTCT	50	0.12274155538098978	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	50	0.12274155538098978	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	49	0.12028672427336999	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT	48	0.1178318931657502	No Hit
TATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATACA	48	0.1178318931657502	No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT	47	0.11537706205813039	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT	47	0.11537706205813039	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	47	0.11537706205813039	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46	0.1129222309505106	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACA	45	0.11046739984289082	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGCTGTCTCTT	44	0.108012568735271	No Hit
CAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCTT	44	0.108012568735271	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGCTGTCTCTTATA	43	0.10555773762765122	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	42	0.10310290652003143	ABI Solid3 Adapter B (100% over 21bp)
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	41	0.10064807541241161	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41	0.10064807541241161	No Hit
CGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCTCT	41	0.10064807541241161	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0024548311076197957	0.0
12	0.0	0.0	0.0	0.0024548311076197957	0.0
13	0.0	0.0	0.0	0.0024548311076197957	0.0
14	0.0	0.0	0.0	0.0024548311076197957	0.0
15	0.0	0.0	0.0	0.024548311076197957	0.0
16	0.0	0.0	0.0	0.03927729772191673	0.0
17	0.0	0.0	0.0	0.07855459544383346	0.0
18	0.0	0.0	0.0	0.09573841319717204	0.0
19	0.0024548311076197957	0.0	0.0	0.12028672427336999	0.0
20	0.0024548311076197957	0.0	0.0	0.13501571091908876	0.0
21	0.0024548311076197957	0.0	0.0	0.1693833464257659	0.0
22	0.0024548311076197957	0.0	0.0	0.2307541241162608	0.0
23	0.0024548311076197957	0.0	0.0	0.33140219952867245	0.0
24	0.0024548311076197957	0.0	0.0	0.4934210526315789	0.0
25	0.0024548311076197957	0.0	0.0	0.5376080125687352	0.0
26	0.0024548311076197957	0.0	0.0	0.6333464257659073	0.0
27	0.0024548311076197957	0.0	0.0	0.6922623723487824	0.0
28	0.0024548311076197957	0.0	0.0	0.9181068342498037	0.0
29	0.0024548311076197957	0.0	0.0	1.4704438334642576	0.0
30	0.0024548311076197957	0.0	0.0	2.5751178318931656	0.0
31	0.0024548311076197957	0.0	0.0	4.728004713275727	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGAC	25	0.005435045	29.6	30
ATTGACT	25	0.005435045	29.6	31
CGTTGAA	35	8.7226514E-4	26.428572	2
ACAGTGG	55	1.8448947E-5	23.545454	25
GGAACAG	65	2.5789086E-6	22.76923	22
GGTATCA	590	0.0	22.262712	1
GGGAACA	110	6.730261E-11	21.863636	21
AACAGTG	60	3.6139456E-5	21.583332	24
TGGGCAG	260	0.0	21.346153	6
GAAGCAT	70	4.9054834E-6	21.142859	23
GAACAGT	70	4.9054834E-6	21.142859	23
TGGGAAC	140	0.0	21.142859	20
GCATGGT	45	0.0037637025	20.555555	26
GACTGTC	45	0.0037637025	20.555555	34
AGCATGG	55	5.0269323E-4	20.181818	25
GGGCAGT	270	0.0	19.87037	7
TGTTCCC	75	8.91122E-6	19.733334	5
AAGCATG	75	8.91122E-6	19.733334	24
GGCAGTC	275	0.0	19.509092	8
GCAGTCG	280	0.0	19.160715	9
>>END_MODULE