##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631543.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7304
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75355969331873	34.0	31.0	34.0	31.0	34.0
2	32.897042716319824	34.0	33.0	34.0	31.0	34.0
3	32.967278203723986	34.0	33.0	34.0	31.0	34.0
4	36.294906900328584	37.0	37.0	37.0	35.0	37.0
5	36.243975903614455	37.0	37.0	37.0	35.0	37.0
6	36.31736035049288	37.0	37.0	37.0	35.0	37.0
7	36.3158543263965	37.0	37.0	37.0	35.0	37.0
8	36.33105147864184	37.0	37.0	37.0	35.0	37.0
9	38.1592278203724	39.0	38.0	39.0	37.0	39.0
10	38.070098576122675	39.0	38.0	39.0	37.0	39.0
11	38.12855969331873	39.0	38.0	39.0	37.0	39.0
12	38.108981380065714	39.0	38.0	39.0	37.0	39.0
13	38.14854874041621	39.0	38.0	39.0	37.0	39.0
14	39.57625958378971	41.0	40.0	41.0	37.0	41.0
15	39.575985761226725	41.0	39.0	41.0	37.0	41.0
16	39.50643483023001	41.0	39.0	41.0	37.0	41.0
17	39.50177984665937	41.0	39.0	41.0	37.0	41.0
18	39.45303943044907	41.0	39.0	41.0	37.0	41.0
19	39.50109529025192	41.0	39.0	41.0	37.0	41.0
20	39.474945235487404	41.0	39.0	41.0	37.0	41.0
21	39.43907447973713	41.0	39.0	41.0	36.0	41.0
22	39.35952902519168	41.0	39.0	41.0	36.0	41.0
23	39.333652792990144	41.0	39.0	41.0	36.0	41.0
24	39.285870755750274	41.0	39.0	41.0	36.0	41.0
25	39.28778751369113	41.0	39.0	41.0	36.0	41.0
26	39.19277108433735	41.0	39.0	41.0	36.0	41.0
27	39.112404162102955	40.0	39.0	41.0	35.0	41.0
28	39.0197152245345	40.0	39.0	41.0	35.0	41.0
29	38.939895947426066	40.0	38.0	41.0	35.0	41.0
30	38.95947426067908	40.0	38.0	41.0	35.0	41.0
31	38.88964950711939	40.0	38.0	41.0	35.0	41.0
32	38.77984665936473	40.0	38.0	41.0	35.0	41.0
33	38.63636363636363	40.0	38.0	41.0	35.0	41.0
34	38.52916210295729	40.0	38.0	41.0	35.0	41.0
35	38.45043811610077	40.0	38.0	41.0	34.0	41.0
36	38.41853778751369	40.0	38.0	41.0	34.0	41.0
37	38.32721796276013	40.0	38.0	41.0	34.0	41.0
38	38.27834063526834	40.0	37.0	41.0	34.0	41.0
39	38.19797371303395	40.0	37.0	41.0	34.0	41.0
40	38.05777656078861	40.0	37.0	41.0	34.0	41.0
41	38.00862541073384	40.0	37.0	41.0	34.0	41.0
42	37.88335158817087	40.0	37.0	41.0	33.0	41.0
43	36.943044906900326	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	3.0
23	3.0
24	4.0
25	6.0
26	17.0
27	19.0
28	31.0
29	36.0
30	51.0
31	86.0
32	102.0
33	123.0
34	169.0
35	261.0
36	444.0
37	763.0
38	1629.0
39	3556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.716319824753555	18.52409638554217	12.855969331872947	25.903614457831324
2	21.12541073384447	19.2223439211391	31.98247535596933	27.6697699890471
3	20.89266155531216	21.39923329682366	28.203723986856517	29.504381161007665
4	15.6763417305586	15.66265060240964	32.434282584885	36.22672508214677
5	17.880613362541073	33.324205914567365	30.531215772179625	18.263964950711937
6	35.14512595837897	32.187842278203725	15.66265060240964	17.004381161007668
7	30.94194961664841	28.573384446878425	19.49616648411829	20.988499452354876
8	28.58707557502738	30.51752464403067	19.249726177437022	21.645673603504928
9	28.285870755750274	14.060788608981381	18.52409638554217	39.12924424972618
10	20.194414019715225	24.260679079956187	28.58707557502738	26.957831325301207
11	38.12979189485213	21.09802847754655	18.68838992332968	22.08378970427163
12	23.64457831325301	24.835706462212485	26.04052573932092	25.47918948521358
13	31.46221248630887	17.278203723986856	24.288061336254106	26.971522453450163
14	24.98630887185104	20.399780941949615	22.234392113910186	32.37951807228915
15	28.806133625410734	24.76725082146769	19.89320920043812	26.533406352683457
16	26.69769989047098	23.808871851040525	22.89156626506024	26.601861993428262
17	26.642935377875137	25.123220153340636	22.343921139101862	25.889923329682368
18	26.820920043811608	21.084337349397593	25.191675794085434	26.90306681270537
19	26.998904709748082	24.096385542168676	23.94578313253012	24.95892661555312
20	27.135815991237678	23.42552026286966	23.69934282584885	25.739320920043813
21	28.73767798466594	22.604052573932094	23.370755750273823	25.28751369112815
22	28.860898138006576	22.494523548740418	22.316538882803943	26.328039430449067
23	26.69769989047098	22.754654983570646	23.08324205914567	27.464403066812704
24	26.642935377875137	23.138006571741514	23.61719605695509	26.601861993428262
25	26.451259583789703	22.604052573932094	23.4118291347207	27.5328587075575
26	26.259583789704273	24.041621029572838	23.42552026286966	26.27327491785323
27	27.87513691128149	22.207009857612267	22.836801752464403	27.08105147864184
28	25.31489594742607	23.274917853231106	24.904162102957283	26.506024096385545
29	24.52081051478642	22.836801752464403	25.506571741511504	27.135815991237678
30	25.780394304490688	23.713033953997808	25.629791894852133	24.876779846659364
31	26.300657174151148	24.219605695509312	23.521358159912374	25.958378970427166
32	24.98630887185104	23.49397590361446	24.644030668127055	26.875684556407446
33	26.06790799561884	22.9052573932092	24.17853231106243	26.848302300109527
34	26.506024096385545	21.330777656078862	25.575027382256298	26.5881708652793
35	25.903614457831324	22.782037239868565	24.288061336254106	27.026286966046005
36	24.370208105147864	22.836801752464403	25.739320920043813	27.053669222343924
37	25.068455640744798	21.371851040525737	26.875684556407446	26.684008762322016
38	24.397590361445783	20.89266155531216	27.286418400876233	27.423329682365825
39	24.342825848849944	21.358159912376777	27.546549835706465	26.75246440306681
40	23.45290251916758	21.56352683461117	28.368017524644028	26.615553121577218
41	22.508214676889377	21.262322015334064	29.1894852135816	27.03997809419496
42	23.43921139101862	21.41292442497262	28.039430449069002	27.10843373493976
43	21.11171960569551	21.276013143483024	29.901423877327492	27.710843373493976
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.5
4	2.0
5	2.0
6	5.0
7	8.0
8	6.0
9	4.0
10	4.5
11	5.0
12	5.0
13	12.5
14	20.0
15	37.0
16	54.0
17	41.5
18	29.0
19	29.0
20	41.5
21	54.0
22	40.0
23	26.0
24	33.5
25	41.0
26	41.0
27	50.5
28	60.0
29	59.0
30	58.0
31	89.0
32	120.0
33	120.0
34	137.5
35	155.0
36	173.0
37	191.0
38	226.0
39	261.0
40	261.0
41	284.0
42	307.0
43	330.0
44	353.0
45	407.0
46	461.0
47	461.0
48	506.5
49	552.0
50	584.0
51	616.0
52	621.0
53	626.0
54	626.0
55	586.5
56	547.0
57	549.5
58	552.0
59	507.5
60	463.0
61	463.0
62	454.5
63	446.0
64	413.0
65	380.0
66	333.0
67	286.0
68	286.0
69	245.0
70	204.0
71	186.5
72	169.0
73	126.5
74	84.0
75	84.0
76	66.0
77	48.0
78	50.5
79	53.0
80	42.0
81	31.0
82	31.0
83	22.0
84	13.0
85	12.5
86	12.0
87	8.5
88	5.0
89	5.0
90	6.0
91	7.0
92	3.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7304.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.40361445783132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.3250735635744	82.50273822562978
2	4.073098962366424	7.2015334063526835
3	1.2389654638376955	3.285870755750274
4	0.5730215270249341	2.026286966046002
5	0.32522843425739506	1.4375684556407449
6	0.20133188787362552	1.067907995618839
7	0.12389654638376955	0.7667031763417306
8	0.04646120489391358	0.32858707557502737
9	0.015487068297971194	0.12322015334063528
>10	0.07743534148985597	1.2595837897042717
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28	0.3833515881708653	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24	0.32858707557502737	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	16	0.21905805038335158	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.16429353778751368	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	12	0.16429353778751368	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	9	0.12322015334063528	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	8	0.10952902519167579	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG	8	0.10952902519167579	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC	8	0.10952902519167579	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.013691128148959474	0.0
23	0.0	0.0	0.0	0.027382256297918947	0.0
24	0.0	0.0	0.0	0.027382256297918947	0.0
25	0.0	0.0	0.0	0.04107338444687842	0.0
26	0.0	0.0	0.0	0.054764512595837894	0.0
27	0.0	0.0	0.0	0.09583789704271632	0.0
28	0.0	0.0	0.0	0.24644030668127054	0.0
29	0.0	0.0	0.0	0.43811610076670315	0.0
30	0.0	0.0	0.0	0.6982475355969332	0.0
31	0.0	0.0	0.0	1.355421686746988	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE