FastQCFastQC Report
Fri 10 Feb 2017
ERR1631542.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631542.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266694
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT114864.306808552123408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT101963.8231081314165296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT83363.1256796178391713No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29901.1211350836539256No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA21220.7956684439844917No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA12660.47470134311233103No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10570.39633437572648805No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10090.37833622053739496No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA10090.37833622053739496No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10090.37833622053739496No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA9890.37083698920860614No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA9080.3404651023270115No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC8040.3014690994173097No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC7780.2917200986898843No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.29097017555700544No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7700.2887204061583688No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA7650.28684559832617157No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT7290.2733469819343517No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT7270.27259705880147284No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA6540.24522486445139371No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.23772563312260495No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT6120.22947647866093726No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT5560.20847863094032865No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC5460.20472901527593423No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA5270.19760474551358487No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT5150.19310520671631157No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG5120.19198032201699325No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4920.18448109068820445No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.18223132128956782No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4730.17735682092585509No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG4650.1743571283943396No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA4420.16573301236623245No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA3810.14286035681342663No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3610.13536112548463783No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.13423624078531948No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC3350.1256121247572124No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT3350.1256121247572124No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT3300.12373731692501518No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC3220.12073762439349965No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT3140.11773793186198415No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3090.11586312402978695No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT3070.11511320089690807No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3030.11361335463115031No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAA3000.11248846993183198No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA2990.11211350836539255No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA2920.10948877740031646ABI Solid3 Adapter B (100% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC2910.10911381583387703No Hit
ATCACCGACTGCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAA2900.10873885426743758ABI Solid3 Adapter B (95% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.10798893113455871No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT2810.10536420016948264No Hit
GCCTCTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTG2780.10423931547016432No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT2720.10198954607152767No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT2670.10011473823933047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGT355.609709E-737.028
GTAAGTT355.609709E-737.029
CGGGTAA401.5939731E-632.37526
TCGGGTA401.5939731E-632.37525
TAAGTTC401.5939731E-632.37530
ATGCCCT303.5921333E-430.83333437
AACGTGG250.005488869329.610
TTAAACC250.005488869329.636
TCATAGC250.005488869329.63
GAATTTC250.005488869329.622
GGGTAAG453.9930965E-628.77777727
TCCTTGT453.9930965E-628.77777719
TTATTGC453.9930965E-628.77777729
TATTGAC1350.027.40740630
TTGGAGC509.056943E-625.9000023
CGAAATT509.056943E-625.90000213
AAATTCC509.056943E-625.90000215
TATTGCT451.3194924E-424.66666630
TTTGGAG451.3194924E-424.6666662
AGTTCCG451.3194924E-424.66666632
Produced by FastQC (version 0.11.5)