##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631542.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	266694
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.878849917883414	34.0	33.0	34.0	31.0	34.0
2	32.93687522028992	34.0	33.0	34.0	31.0	34.0
3	32.91443002092286	34.0	33.0	34.0	31.0	34.0
4	36.29606965286058	37.0	37.0	37.0	35.0	37.0
5	36.322009494026865	37.0	37.0	37.0	35.0	37.0
6	36.44684919795721	37.0	37.0	37.0	35.0	37.0
7	36.44709292297539	37.0	37.0	37.0	35.0	37.0
8	36.490817191237895	37.0	37.0	37.0	35.0	37.0
9	38.2507105521684	39.0	39.0	39.0	37.0	39.0
10	38.213090658207534	39.0	39.0	39.0	37.0	39.0
11	38.28670311293092	39.0	39.0	39.0	37.0	39.0
12	38.280268772450825	39.0	39.0	39.0	37.0	39.0
13	38.289312845433344	39.0	39.0	39.0	37.0	39.0
14	39.58205658919961	41.0	39.0	41.0	37.0	41.0
15	39.508466632170204	41.0	39.0	41.0	37.0	41.0
16	39.39615064455893	40.0	39.0	41.0	37.0	41.0
17	39.48166437940111	40.0	39.0	41.0	37.0	41.0
18	39.6609972477821	41.0	39.0	41.0	38.0	41.0
19	39.7372944273212	41.0	40.0	41.0	38.0	41.0
20	39.786208163663225	41.0	40.0	41.0	38.0	41.0
21	39.77635792331286	41.0	40.0	41.0	37.0	41.0
22	39.695422469196906	41.0	40.0	41.0	37.0	41.0
23	39.594527810899386	41.0	40.0	41.0	36.0	41.0
24	39.54695643696521	41.0	40.0	41.0	36.0	41.0
25	39.51694076357173	41.0	40.0	41.0	36.0	41.0
26	39.3060511297592	41.0	39.0	41.0	35.0	41.0
27	39.13936946462987	41.0	39.0	41.0	35.0	41.0
28	38.978177236833226	41.0	38.0	41.0	35.0	41.0
29	38.72579810569417	40.0	38.0	41.0	35.0	41.0
30	38.39546446489235	40.0	37.0	41.0	35.0	41.0
31	38.00607812699198	40.0	35.0	41.0	35.0	41.0
32	37.66671166205464	40.0	35.0	41.0	35.0	41.0
33	37.385539232228695	39.0	35.0	41.0	35.0	41.0
34	37.16690289245352	39.0	35.0	41.0	34.0	41.0
35	36.91837836621746	39.0	35.0	41.0	33.0	41.0
36	36.64987213810584	39.0	35.0	41.0	33.0	41.0
37	36.36712486970085	38.0	35.0	41.0	33.0	41.0
38	36.07711084613827	38.0	35.0	41.0	33.0	41.0
39	35.69495376723886	37.0	35.0	41.0	31.0	41.0
40	35.30080916706038	37.0	35.0	41.0	29.0	41.0
41	34.82013468619466	37.0	35.0	41.0	23.0	41.0
42	34.32223822058239	36.0	35.0	41.0	20.0	41.0
43	33.32466422191725	35.0	34.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	6.0
12	10.0
13	9.0
14	4.0
15	11.0
16	6.0
17	7.0
18	19.0
19	16.0
20	48.0
21	57.0
22	123.0
23	204.0
24	294.0
25	456.0
26	741.0
27	1199.0
28	1877.0
29	2637.0
30	3559.0
31	4172.0
32	4769.0
33	5937.0
34	8169.0
35	11379.0
36	18729.0
37	57171.0
38	55032.0
39	90048.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.769751100512195	14.000689929282247	16.40944303208921	20.82011593811634
2	18.920185680967702	23.217995155496563	34.12675200791919	23.735067155616548
3	20.134686194665047	26.43704020337915	30.774220642384154	22.654052959571644
4	12.147629867938537	19.16278581445402	30.25527383443197	38.43431048317548
5	11.871658155039109	42.86785604475541	28.15061456200739	17.109871238198085
6	26.234560957501856	31.289792796238387	21.909379288622915	20.56626695763684
7	28.712306988533676	34.9197957209386	17.156741434003013	19.211155856524705
8	38.13959069195407	26.954112203499143	15.930992073312487	18.9753050312343
9	33.211470824240514	11.360585539982152	17.34872175602001	38.07922187975733
10	22.090110763646724	30.01529843191073	20.85536232536165	27.039228479080897
11	31.212550713551863	28.806797303276415	14.303283913398875	25.67736806977285
12	25.108551373484218	33.53393777137843	23.623703570384038	17.733807284753315
13	36.24640974300134	19.225779357615842	24.978064748363295	19.54974615101952
14	24.254388925135174	23.295612199749527	27.723908299399312	24.72609057571599
15	32.614157048902484	24.735089653310535	23.069510375186546	19.581242922600435
16	21.2903177424314	25.59562644828905	29.4179846565727	23.696071152706846
17	17.578198234680944	32.095585202516745	28.30097415014961	22.025242412652705
18	19.93895625698366	22.08636114798233	33.82415802380256	24.15052457123145
19	19.43763264265413	29.248127066975638	36.35102402003795	14.96321627033229
20	22.51494221842261	22.292215047957583	37.95098502403504	17.241857709584767
21	25.708864841353762	21.414430020922858	35.45186618371617	17.424838954007214
22	24.126527030979325	22.722295964663623	31.51514469766849	21.636032306688566
23	19.57036903717369	26.27055726788004	32.551538467307104	21.607535227639165
24	18.777325324154273	26.161068490479728	36.41551740946553	18.64608877590047
25	17.898415412420228	25.83035238888014	33.467569574118656	22.80366262458098
26	21.520169182658776	24.58473006516832	33.51256496209138	20.382535790081516
27	19.014301034143998	25.35490112263493	32.4982939248727	23.13250391834837
28	17.25498136441015	26.692389030124414	35.48973730192655	20.562892303538888
29	17.571823888051476	27.379693581407906	36.811476823625576	18.237005706915042
30	16.53130554118203	28.612942173427225	38.694158848718004	16.161593436672742
31	17.411340337615393	30.242150179606593	34.05250961776418	18.293999865013834
32	16.10197454760887	29.882187075824728	37.77962758817221	16.23621078839419
33	17.631442777115346	27.87764254163948	37.32179951554966	17.169115165695516
34	18.46535730087666	29.65796005909394	32.262068138015856	19.614614502013545
35	16.619796470861736	31.566514432270694	32.786264407898194	19.02742468896938
36	14.460017848170562	31.338537799875514	34.40234875925218	19.79909559270175
37	15.847000682430052	31.822613182148828	33.736791978822176	18.59359415659895
38	16.27708159913609	28.10674405873398	35.76608397639242	19.850090365737515
39	17.751805439942405	28.131116560552545	35.104276811626804	19.012801187878242
40	16.364822605682917	27.77790276496659	33.24709217305226	22.61018245629823
41	13.516239585442491	29.425108926335053	35.81595386472887	21.24269762349359
42	14.18067148117318	28.141990445979285	35.049532422926646	22.62780564992088
43	12.989418584595077	31.003697121045093	36.230661357210884	19.776222937148944
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	46.0
2	81.0
3	376.5
4	672.0
5	672.0
6	963.0
7	1254.0
8	1306.0
9	1358.0
10	2147.5
11	2937.0
12	2937.0
13	5581.0
14	8225.0
15	14170.0
16	20115.0
17	17706.0
18	15297.0
19	15297.0
20	17118.0
21	18939.0
22	12605.0
23	6271.0
24	5563.0
25	4855.0
26	4855.0
27	4580.5
28	4306.0
29	4515.5
30	4725.0
31	4627.0
32	4529.0
33	4529.0
34	4214.5
35	3900.0
36	3655.5
37	3411.0
38	3710.5
39	4010.0
40	4010.0
41	4656.5
42	5303.0
43	6812.0
44	8321.0
45	11011.5
46	13702.0
47	13702.0
48	20522.5
49	27343.0
50	25843.0
51	24343.0
52	18676.0
53	13009.0
54	13009.0
55	10607.5
56	8206.0
57	8143.5
58	8081.0
59	8188.0
60	8295.0
61	8295.0
62	8409.5
63	8524.0
64	8387.0
65	8250.0
66	8055.0
67	7860.0
68	7860.0
69	7332.5
70	6805.0
71	5992.5
72	5180.0
73	4422.0
74	3664.0
75	3664.0
76	3019.5
77	2375.0
78	1857.5
79	1340.0
80	1018.5
81	697.0
82	697.0
83	508.0
84	319.0
85	222.0
86	125.0
87	80.0
88	35.0
89	35.0
90	25.0
91	15.0
92	10.0
93	5.0
94	3.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	266694.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.29892118889552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.3298626263391	34.82732350475564
2	8.432071685675249	6.964709280116391
3	2.336835189137485	2.895263169228802
4	1.1556534489075116	1.9090896283242649
5	0.7113330775968649	1.4688644355363711
6	0.4740465968202491	1.1746567825166156
7	0.3634730488713172	1.050773135973687
8	0.24397084311507836	0.8060586097758411
9	0.1971675365695867	0.7328525898416433
>10	1.438923259921597	11.665997939148275
>50	0.1613187273630462	4.60890601182954
>100	0.12845750512242568	10.39440523324633
>500	0.01692850842698633	4.8536012092096215
>1k	0.006970562293464961	4.302522949563723
>5k	9.957946133521372E-4	3.4282002779165737
>10k+	0.0019915892267042745	8.916775243016692
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11486	4.306808552123408	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10196	3.8231081314165296	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8336	3.1256796178391713	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2990	1.1211350836539256	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	2122	0.7956684439844917	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	1266	0.47470134311233103	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1057	0.39633437572648805	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1009	0.37833622053739496	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	1009	0.37833622053739496	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1009	0.37833622053739496	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	989	0.37083698920860614	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	908	0.3404651023270115	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	804	0.3014690994173097	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	778	0.2917200986898843	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	776	0.29097017555700544	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	770	0.2887204061583688	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	765	0.28684559832617157	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	729	0.2733469819343517	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	727	0.27259705880147284	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	654	0.24522486445139371	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	634	0.23772563312260495	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	612	0.22947647866093726	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	556	0.20847863094032865	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	546	0.20472901527593423	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	527	0.19760474551358487	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	515	0.19310520671631157	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	512	0.19198032201699325	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	492	0.18448109068820445	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	486	0.18223132128956782	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	473	0.17735682092585509	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	465	0.1743571283943396	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	442	0.16573301236623245	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA	381	0.14286035681342663	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	361	0.13536112548463783	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	358	0.13423624078531948	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	335	0.1256121247572124	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	335	0.1256121247572124	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	330	0.12373731692501518	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	322	0.12073762439349965	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	314	0.11773793186198415	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	309	0.11586312402978695	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	307	0.11511320089690807	No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	303	0.11361335463115031	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAA	300	0.11248846993183198	No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA	299	0.11211350836539255	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	292	0.10948877740031646	ABI Solid3 Adapter B (100% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	291	0.10911381583387703	No Hit
ATCACCGACTGCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAA	290	0.10873885426743758	ABI Solid3 Adapter B (95% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	288	0.10798893113455871	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	281	0.10536420016948264	No Hit
GCCTCTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTG	278	0.10423931547016432	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	272	0.10198954607152767	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	267	0.10011473823933047	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.7496156643943994E-4	0.0
11	0.0	0.0	0.0	0.0011248846993183199	0.0
12	0.0	0.0	0.0	0.0018748078321971999	0.0
13	0.0	0.0	0.0	0.00262473096507608	0.0
14	0.0	0.0	0.0	0.0044995387972732795	0.0
15	0.0	0.0	0.0	0.0074992313287887994	0.0
16	0.0	0.0	0.0	0.014248539524698718	0.0
17	0.0	0.0	0.0	0.021747770853487516	0.0
18	0.0	0.0	0.0	0.025872348084321355	0.0
19	0.0	0.0	0.0	0.031871733147352393	0.0
20	0.0	0.0	0.0	0.03637127194462567	0.0
21	0.0	0.0	0.0	0.050994773035763837	0.0
22	0.0	0.0	0.0	0.07761704425296408	0.0
23	0.0	0.0	0.0	0.11211350836539255	0.0
24	0.0	0.0	0.0	0.15373424224017038	0.0
25	0.0	0.0	0.0	0.1698575895970663	0.0
26	0.0	0.0	0.0	0.1912303988841144	0.0
27	0.0	0.0	0.0	0.2261018245629823	0.0
28	0.0	0.0	0.0	0.3239667934036761	0.0
29	0.0	0.0	0.0	0.524196269882337	0.0
30	0.0	0.0	0.0	0.8091670603763115	0.0
31	0.0	0.0	0.0	1.427478683434948	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTAAGT	35	5.609709E-7	37.0	28
GTAAGTT	35	5.609709E-7	37.0	29
CGGGTAA	40	1.5939731E-6	32.375	26
TCGGGTA	40	1.5939731E-6	32.375	25
TAAGTTC	40	1.5939731E-6	32.375	30
ATGCCCT	30	3.5921333E-4	30.833334	37
AACGTGG	25	0.0054888693	29.6	10
TTAAACC	25	0.0054888693	29.6	36
TCATAGC	25	0.0054888693	29.6	3
GAATTTC	25	0.0054888693	29.6	22
GGGTAAG	45	3.9930965E-6	28.777777	27
TCCTTGT	45	3.9930965E-6	28.777777	19
TTATTGC	45	3.9930965E-6	28.777777	29
TATTGAC	135	0.0	27.407406	30
TTGGAGC	50	9.056943E-6	25.900002	3
CGAAATT	50	9.056943E-6	25.900002	13
AAATTCC	50	9.056943E-6	25.900002	15
TATTGCT	45	1.3194924E-4	24.666666	30
TTTGGAG	45	1.3194924E-4	24.666666	2
AGTTCCG	45	1.3194924E-4	24.666666	32
>>END_MODULE